Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pNTI194
(Plasmid #84266)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 84266 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA5/FRT/TO
  • Backbone manufacturer
    Invitrogen
  • Vector type
    Mammalian Expression
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    RPL1
  • Alt name
    RPL10A
  • Alt name
    ribosomal protein L10a
  • Species
    H. sapiens (human); (*see below)
  • GenBank ID
    NM_007104.4 NP_009035.3
  • Entrez Gene
    RPL10A (a.k.a. CSA19, Csa-19, L10A, NEDD6)
  • Promoter CMV
  • Tags / Fusion Proteins
    • Gly/Ser linker (C terminal on insert)
    • TEV site (C terminal on insert)
    • Avi tag (C terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    humanized birA
  • Species
    H. sapiens (human); E. coli
  • Mutation
    R33A, A34E (*see below)
  • Promoter P2A peptide
  • Tag / Fusion Protein
    • Flag (C terminal on insert)

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

*Two mutations, R33A and A34E, were introduced into the biotin ligase order to eliminate an unwanted DNA-binding activity without affecting biotinylation, as described in Buoncristiani et al., 1986.

Note that the RPL10A protein sequences are identical in human, mouse, and rat, but the human DNA sequence is used in this plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNTI194 was a gift from Nicholas Ingolia (Addgene plasmid # 84266 ; http://n2t.net/addgene:84266 ; RRID:Addgene_84266)
  • For your References section:

    Ribosome profiling reveals pervasive translation outside of annotated protein-coding genes. Ingolia NT, Brar GA, Stern-Ginossar N, Harris MS, Talhouarne GJ, Jackson SE, Wills MR, Weissman JS. Cell Rep. 2014 Sep 11;8(5):1365-79. doi: 10.1016/j.celrep.2014.07.045. Epub 2014 Aug 21. 10.1016/j.celrep.2014.07.045 PubMed 25159147