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pcDNA3-GFP-IMP2-2
(Plasmid #42175)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 42175 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1/CT-GFP TOPO
  • Backbone manufacturer
    invitrogen
  • Backbone size w/o insert (bp) 6157
  • Total vector size (bp) 7828
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Top10
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Insulin-like growth factor 2 mRNA binding protein 2-2
  • Alt name
    IMP2-2, p62, IGF2BP2-2
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1671
  • GenBank ID
    NM_001007225.1
  • Entrez Gene
    IGF2BP2 (a.k.a. IMP-2, IMP2, VICKZ2)
  • Promoter T7
  • Tag / Fusion Protein
    • GFP (C terminal on insert)

Cloning Information

  • Cloning method TOPO Cloning
  • 5′ sequencing primer TAATACGACTCACTATAGGG
  • 3′ sequencing primer GGGTAAGCTTTCCGTATGTAGC
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3-GFP-IMP2-2 was a gift from Alexandra Kiemer (Addgene plasmid # 42175 ; http://n2t.net/addgene:42175 ; RRID:Addgene_42175)
  • For your References section:

    IGF2 mRNA binding protein p62/IMP2-2 in hepatocellular carcinoma: antiapoptotic action is independent of IGF2/PI3K signaling. Kessler SM, Pokorny J, Zimmer V, Laggai S, Lammert F, Bohle RM, Kiemer AK. Am J Physiol Gastrointest Liver Physiol. 2013 Feb 15;304(4):G328-36. doi: 10.1152/ajpgi.00005.2012. Epub 2012 Dec 20. 10.1152/ajpgi.00005.2012 PubMed 23257922