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pUS252
(Plasmid #127674)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 127674 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pK18
  • Backbone size w/o insert (bp) 2620
  • Total vector size (bp) 3359
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Based on what we know from other high-copy GFP-expressing vectors, there is a chance of vector instability (deletions / loss of GFP gene), although we havent seen this for this plasmid. Reducing growth temperature to 30 C can help to prevent this, since it lowers the copy number of the plasmid.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Free Use GFP (fuGFP)
  • Species
    Synthetic
  • Insert Size (bp)
    739
  • Promoter lac

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI, EcoRI, XbaI, XhoI, NdeI (not destroyed)
  • 3′ cloning site HindIII, PstI, SphI, NheI (not destroyed)
  • 5′ sequencing primer M13 rev
  • 3′ sequencing primer M13 fwd
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

The creators (Nick Coleman & Mark Somerville) request that their work is cited once published. Please follow Addgene's "How to Cite" guidelines below.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pUS252 was a gift from Nicholas Coleman (Addgene plasmid # 127674 ; http://n2t.net/addgene:127674 ; RRID:Addgene_127674)