Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more


(Plasmid #114040)


Item Catalog # Description Quantity Price (USD)
Plasmid 114040 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Vector type
    Mammalian Expression
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    H. sapiens (human)
  • Entrez Gene
    MIS12 (a.k.a. 2510025F08Rik, KNTC2AP, MTW1, hMis12)
  • Promoter CMV
  • Tag / Fusion Protein
    • FRB-FLAG-tagRFP (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unknown (unknown if destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

T2098L mutant of FKBP-rapamycin binding domain of human FRAP. Puromycin is expressed via IRES sequence

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MIS12-FRB-FLAG was a gift from Geert Kops (Addgene plasmid # 114040 ; ; RRID:Addgene_114040)
  • For your References section:

    Conditional targeting of MAD1 to kinetochores is sufficient to reactivate the spindle assembly checkpoint in metaphase. Kuijt TE, Omerzu M, Saurin AT, Kops GJ. Chromosoma. 2014 Oct;123(5):471-80. doi: 10.1007/s00412-014-0458-9. Epub 2014 Apr 4. 10.1007/s00412-014-0458-9 PubMed 24695965