MIS12-FRB-FLAG
(Plasmid
#114040)
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PurposeKinetochore localised FRB tagged with FLAG
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 114040 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepcDNA3.0
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Vector typeMammalian Expression
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMIS12
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SpeciesH. sapiens (human)
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Entrez GeneMIS12 (a.k.a. 2510025F08Rik, KNTC2AP, MTW1, hMis12)
- Promoter CMV
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Tag
/ Fusion Protein
- FRB-FLAG-tagRFP (C terminal on insert)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
T2098L mutant of FKBP-rapamycin binding domain of human FRAP. Puromycin is expressed via IRES sequence
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MIS12-FRB-FLAG was a gift from Geert Kops (Addgene plasmid # 114040 ; http://n2t.net/addgene:114040 ; RRID:Addgene_114040) -
For your References section:
Conditional targeting of MAD1 to kinetochores is sufficient to reactivate the spindle assembly checkpoint in metaphase. Kuijt TE, Omerzu M, Saurin AT, Kops GJ. Chromosoma. 2014 Oct;123(5):471-80. doi: 10.1007/s00412-014-0458-9. Epub 2014 Apr 4. 10.1007/s00412-014-0458-9 PubMed 24695965
