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Conditional targeting of MAD1 to kinetochores is sufficient to reactivate the spindle assembly checkpoint in metaphase.
Kuijt TE, Omerzu M, Saurin AT, Kops GJ
Chromosoma. 2014 Oct;123(5):471-80. doi: 10.1007/s00412-014-0458-9. Epub 2014 Apr 4.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
114037pcDNA5-eYFP-FKBP-MAD1-WTChemical induction of MAD1 localisation to a specific site in the cell (by rapamycin & FRB)
114038pcDNA5-eYFP-FKBP-MAD1-AAChemical induction of MAD1 localisation to a specific site in the cell (by rapamycin & FRB)
114039MIS12-FRB-tagRFPKinetochore localised FRB tagged with FLAG and tagRFP
114040MIS12-FRB-FLAGKinetochore localised FRB tagged with FLAG

Antibodies from Article