PurposeKinetochore localised FRB tagged with FLAG and tagRFP
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||114039||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Copy numberHigh Copy
SpeciesH. sapiens (human)
Entrez GeneMIS12 (a.k.a. 2510025F08Rik, KNTC2AP, MTW1, hMis12)
- Promoter CMV
/ Fusion Protein
- FRB-FLAG-tagRFP (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
T2098L mutant of FKBP-rapamycin binding domain of human FRAP. Puromycin is expressed via IRES sequence
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MIS12-FRB-tagRFP was a gift from Geert Kops (Addgene plasmid # 114039 ; http://n2t.net/addgene:114039 ; RRID:Addgene_114039)
For your References section:Conditional targeting of MAD1 to kinetochores is sufficient to reactivate the spindle assembly checkpoint in metaphase. Kuijt TE, Omerzu M, Saurin AT, Kops GJ. Chromosoma. 2014 Oct;123(5):471-80. doi: 10.1007/s00412-014-0458-9. Epub 2014 Apr 4. 10.1007/s00412-014-0458-9 PubMed 24695965