MAD2
(Plasmid
#99512)
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Purposeto express MAD2 in mammalian system
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Depositing Labs
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Depositing OrganizationUniversity of Hong Kong
Located in Hong Kong Special Administrative Region of China -
Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 99512 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 99512-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 99512-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepIND(SP1)
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Backbone manufacturerInvitrogen
- Backbone size w/o insert (bp) 5081
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameMAD2
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SpeciesH. sapiens (human)
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Insert Size (bp)618
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Entrez GeneMAD2L1 (a.k.a. HSMAD2, MAD2)
- Promoter heat shock
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XbaI (not destroyed)
- 5′ sequencing primer unknown
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 99512-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 99512-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
MAD2 was a gift from Kuan-Teh Jeang & Dong-Yan Jin (Addgene plasmid # 99512 ; http://n2t.net/addgene:99512 ; RRID:Addgene_99512) -
For your References section:
Human T cell leukemia virus type 1 oncoprotein Tax targets the human mitotic checkpoint protein MAD1. Jin DY, Spencer F, Jeang KT. Cell. 1998 Apr 3;93(1):81-91. S0092-8674(00)81148-4 [pii] PubMed 9546394
