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CasX enzymes comprise a distinct family of RNA-guided genome editors.
Liu JJ, Orlova N, Oakes BL, Ma E, Spinner HB, Baney KLM, Chuck J, Tan D, Knott GJ, Harrington LB, Al-Shayeb B, Wagner A, Brotzmann J, Staahl BT, Taylor KL, Desmarais J, Nogales E, Doudna JA
Nature. 2019 Feb 4. pii: 10.1038/s41586-019-0908-x. doi: 10.1038/s41586-019-0908-x.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
123120pBLO 64.1E. coli expression vector for active deltaproteobacteria CasX
123121pBLO ddd64.1E. coli expression vector for catalytically inactivated deltaproteobacteria CasX
123122pBLO2 casXE. coli expression vector for CasX sgRNA
123123pBLO 62.4Human expression vector for deltaproteobacteria CasX and sgRNA
123124pBLO 62.5Human expression vector for planctomyces CasX and sgRNA
126418pET 2C-T10-dCasXE. coli expression vector for inactive casX (triple mutations)--Used for CasX protein purification
126419PBLO CasX-gRNAE. coli vector for CasX gRNA --- Used for In Vitro Transcription

Antibodies from Article