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CasX enzymes comprise a distinct family of RNA-guided genome editors.
Liu JJ, Orlova N, Oakes BL, Ma E, Spinner HB, Baney KLM, Chuck J, Tan D, Knott GJ, Harrington LB, Al-Shayeb B, Wagner A, Brotzmann J, Staahl BT, Taylor KL, Desmarais J, Nogales E, Doudna JA
Nature. 2019 Feb 4. pii: 10.1038/s41586-019-0908-x. doi: 10.1038/s41586-019-0908-x.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
123120pBLO 64.1E. coli expression vector for active deltaproteobacteria CasX
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123121pBLO ddd64.1E. coli expression vector for catalytically inactivated deltaproteobacteria CasX
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123122pBLO2 casXE. coli expression vector for CasX sgRNA
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123123pBLO 62.4Human expression vector for deltaproteobacteria CasX and sgRNA
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123124pBLO 62.5Human expression vector for planctomyces CasX and sgRNA
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126418pET 2C-T10-dCasXE. coli expression vector for inactive casX (triple mutations)--Used for CasX protein purification
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126419PBLO CasX-gRNAE. coli vector for CasX gRNA --- Used for In Vitro Transcription
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