PBLO CasX-gRNA
(Plasmid
#126419)
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PurposeE. coli vector for CasX gRNA --- Used for In Vitro Transcription
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 126419 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepBLO
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Vector typeCRISPR ; in vitro transcription
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namecasX gRNA
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gRNA/shRNA sequenceACATCTGGCGCGTTTATTCCATTACTTTGGAGCCAGTCCCAGCGACTATGTCGTATGGACGAAGCGCTTATTTATCGGAGAgaaaCCGATAAGTAAAACGCATCAAAG
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PBLO CasX-gRNA was a gift from Jennifer Doudna (Addgene plasmid # 126419 ; http://n2t.net/addgene:126419 ; RRID:Addgene_126419) -
For your References section:
CasX enzymes comprise a distinct family of RNA-guided genome editors. Liu JJ, Orlova N, Oakes BL, Ma E, Spinner HB, Baney KLM, Chuck J, Tan D, Knott GJ, Harrington LB, Al-Shayeb B, Wagner A, Brotzmann J, Staahl BT, Taylor KL, Desmarais J, Nogales E, Doudna JA. Nature. 2019 Feb 4. pii: 10.1038/s41586-019-0908-x. doi: 10.1038/s41586-019-0908-x. 10.1038/s41586-019-0908-x PubMed 30718774
Map uploaded by the depositor.
