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178,320 results

Showing: 9741 - 9760 of 178320 results

  3. pJR101

    Plasmid
    #187241
    Purpose
    Lentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-GFP marker expression
    Depositor
    Marco Jost, Jonathan Weissman
    Publication
    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75
    Insert
    Lentiviral sgRNA vector for Perturb-seq with mU6 promoter
    Use
    Lentiviral
    Available since
    Oct. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    187241-plasmid-map-sequence-id-374769

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  4. pCMV-T7-ABE8e-nSpG-P2A-EGFP (KAC984)

    Plasmid
    #185911
    Purpose
    pCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nickase SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized ABE8e-nSpG-P2A-EGFP
    Use
    In vitro transcription; t7 promoter
    Tags
    BPNLS and BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpG=D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T133…
    Promoter
    CMV and T7
    Available since
    July 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    185911-plasmid-map-sequence-id-367364

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  5. pcDNA3-ExRai-AktAR2

    Plasmid
    #184047
    Purpose
    Improved excitation-ratiometric biosensor for monitoring Akt/Protein Kinase B activity in living cells.
    Depositor
    Jin Zhang
    Publication
    Chen et al ACS Cent Sci. 2021 Dec 22;7(12):2009-202
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ExRai-AktAR2
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    184047-plasmid-map-sequence-id-363388

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  7. pGL4.10/RSV_SF3B3-miniXon_Luciferase

    Plasmid
    #174660
    Purpose
    Plasmid containing the SF3B3-miniXon/Firefly luciferase cassette. Translation of Firefly luciferase is controlled by splicing modulation of the SF3B3-miniXon by LMI070.
    Depositor
    Beverly L. Davidson
    Publication
    Monteys et al Nature. 2021 Jul 28. pii: 10.1038/s41586
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    miniXon cassette
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    Kozak ATG initiation sequence in middle exon
    Promoter
    RSV
    Available since
    Jan. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    174660-plasmid-map-sequence-id-351622

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  8. pTwist-SARS-CoV-2 Δ18 B.1.617.2v1

    Plasmid
    #179905
    Purpose
    Encodes SARS-CoV-2 B.1.617.2v1 Spike Protein lacking 18 C-terminal amino acids for pseudovirus production
    Depositor
    Alejandro Balazs
    Publication
    Garcia-Beltran et al Cell. 2022 Jan 6. pii: S0092-8674(21)014
    Insert
    SARS-CoV-2 B.1.617.2v1 Spike truncated to remove 18 amino acids (S Severe acute respiratory syndrome coronavirus 2)
    Expression
    Mammalian
    Mutation
    T19R; G142D; E156G; Δ157-158; L452R; T478K; D614G…
    Promoter
    CAG
    Available since
    Jan. 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    179905-plasmid-map-sequence-id-351283

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  9. Luc-noPS

    Plasmid
    #177940
    Purpose
    Transient mammalian expression of Luc2 (firefly luciferase). Lacks packaging sequence and is used as a negative control transfer plasmid for SARS-CoV-2 virus-like particles.
    Depositor
    Jennifer Doudna
    Publication
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Luc2
    Expression
    Mammalian
    Available since
    Nov. 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    177940-plasmid-map-sequence-id-348112

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  16. pC1-mitoRexYFP

    Plasmid
    #60246
    Purpose
    Mitochondria-targeted genetically encoded fluorescent indicator for measuring NAD/NADH ratio.
    Depositor
    Vsevolod Belousov
    Publication
    Bilan et al Biochim Biophys Acta. 2013 Nov 25. pii:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mitoRexYFP
    Tags
    Mitochondrial targeting signal
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    60246-plasmid-map-sequence-id-317300

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  17. pcDNA3.1-Gβγ-BERKY3

    Plasmid
    #158219
    Purpose
    BRET biosensor for detection of free Gβγ in mammalian expression vector pcDNA3.1(+)
    Depositor
    Mikel Garcia-Marcos
    Publication
    Maziarz et al Cell. 2020 Jun 27. pii: S0092-8674(20)30
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Lyn11-Nluc-ER/K linker-3xYFP-GRK3ct
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 17, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    158219-plasmid-map-sequence-id-311194

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Showing: 9741 - 9760 of 178320 results