166,276 results

1. pcDNA3.1-Cyto-iPASnFR-H-mScarlet3-3xFLAG

   Plasmid

   #225241

   Purpose

   Mammalian expression of iPASnFR-H in the cytosol

   Depositor

   Loren Looger

   Article

   Tamura et al bioRxiv 2024.08.21.609037

   Insert

   iPASnFR-H
   

   Tags

   3xFLAG and mScarlet3

   Expression

   Mammalian

   Available Since

   Sept. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pC0055-CMV-dPspCas13b-GS-ADAR2DD(E488Q/T375G)-delta-984-1090

   Plasmid

   #103871

   Purpose

   dPspCas13b-ADAR2DD(E488Q/T375G) fusion that can be used to selectively edit adenosine to inosine in RNA when used in conjuction with a guide RNA. Cas13b is truncated to allow for expression by AAV

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Inserts

   PspCas13b
   huADAR2DD (ADARB1 Human)
   

   Use

   CRISPR

   Tags

   GS and HIV NES

   Expression

   Mammalian

   Mutation

   Changed histidne 133 to alanine and histidine 105…

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB_TRE3G_scFV-sfGFP-Dnmt3a3lCD

   Plasmid

   #235576

   Purpose

   Dox-inducible expression of the catalytic-domain (CD) of epigenetic effector Dnmt3a3L fused with scFV to programme DNA methylation

   Depositor

   Jamie Hackett

   Article

   Policarpi et al Nat Genet. 2024 Jun;56(6):1168-1180. doi

   Insert

   Dnmt3a-Dnmt3L (Dnmt3a, Dnmt3l Mouse)
   

   Use

   CRISPR

   Available Since

   April 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCLIP-tag (m) Vector

   Plasmid

   #101136

   Purpose

   Expression of protein of interest with CLIP-Tag in Mammalian cells

   Depositor

   New England Biolabs, Ana Egana

   Type

   Empty backbone

   Tags

   CLIP-tag (CLIP10)

   Expression

   Mammalian

   Available Since

   Sept. 27, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pAAV-hSyn-hChR2(H134R)-EYFP

   Plasmid

   #26973

   Purpose

   AAV expression of humanized ChR2 with H134R mutation fused to EYFP driven by human Synapsin I promoter for optogenetic excitation

   Depositor

   Karl Deisseroth

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, and AAV9

   Insert

   hChR2(H134R)
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   H134R

   Promoter

   hSyn

   Available Since

   Feb. 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. Tn7 integration plasmid

   Plasmid

   #154134

   Purpose

   Suicidal integration plasmid with the Tn7 arms, streptomycin and kanamycin resistance genes and the restriction site to clone the barcode-carrying cassette.

   Depositor

   Shimon Bershtein

   Article

   Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038

   Inserts

   left and right end of Tn7 arm
   Spectinomycin resistant gene
   

   Expression

   Bacterial

   Promoter

   Spectinomycin resistance gene promoter and no

   Available Since

   July 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAV:ITR-EF1aCore-SaCas9-dual(U6-sgRNA(backbone))-ITR

   Plasmid

   #207878

   Purpose

   AAV vector encoding EF1a core promoter-driven SaCas9 and two sgRNA cloning sites w/ the engineered Sa Guide scaffold variant (BbsI and PaqCI for sgRNA cloning).

   Depositor

   Yeh-Hsing Lao

   Article

   Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

   Type

   Empty backbone

   Use

   AAV and CRISPR

   Expression

   Mammalian

   Available Since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMOS008E: Entry vector for: GCaMP6F calcium sensor (cytosolic)

   Plasmid

   #163064

   Purpose

   Entry vector for: GCaMP6F calcium sensor (cytosolic)

   Depositor

   Adam Cohen

   Article

   Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

   Insert

   GCaMP6F
   

   Use

   Gateway entry vector

   Available Since

   March 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJH001

   Plasmid

   #67641

   Purpose

   High copy Cas9 expression vector with LEU2 marker for yeast transformation

   Depositor

   John Wyrick

   Article

   Laughery et al Yeast. 2015 Dec;32(12):711-20. doi: 10.1

   Inserts

   Cas9
   Empty
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   Aug. 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTRE3G-human cGAS-HA

    Plasmid

    #186842

    Purpose

    Doxycycline-dependent expression of human cGAS gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Mosallanejad et al Sci Immunol. 2023 Jan 20;8(79):eabp9765.

    Insert

    Human cGAS with C-terminal HA tag (Adamts1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Promoter

    TRE3G

    Available Since

    Oct. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNAKv10.1mVenus

    Plasmid

    #85705

    Purpose

    Kv10.1 fused mVenus

    Depositor

    Luis Pardo

    Article

    Chen et al PLoS One. 2011 May 3;6(5):e19257. doi: 1

    Insert

    KCNH1 (KCNH1 Human)
    

    Tags

    mVenus

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST17-UbE2L3

    Plasmid

    #15795

    Depositor

    Wade Harper

    Article

    Jin et al Nature. 2007 Jun 28. 447(7148):1135-8.

    Insert

    Ubiquitin conjugating enzyme UbE2L3 (UBE2L3 Human)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Sept. 28, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGRG36

    Plasmid

    #16666

    Depositor

    Nancy Craig

    Article

    McKenzie et al BMC Microbiol. 2006 Apr 28;6:39. doi: 10

    Insert

    mTn7::MCS
    

    Expression

    Bacterial

    Available Since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUC18-mini-Tn7

    Plasmid

    #64953

    Purpose

    mini-Tn7 base vector

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Methods. 2005 Jun;2(6):443-8.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Aug. 14, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. CGBE1-NG (pBM1246)

    Plasmid

    #140256

    Purpose

    CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_NG-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    eUNG-BE4max(R33A)_NG-ΔUGI
    

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1, NG mutations in SpCas9, D10A in…

    Promoter

    CMV

    Available Since

    Aug. 5, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti CMV Blast empty (w263-1)

    Plasmid

    #17486

    Purpose

    3rd gen lentiviral empty control vector, CMV promoter, Blast 

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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17. METTL3 shRNA 1 tet pLKO puro

    Plasmid

    #162985

    Purpose

    Tet-inducible shRNA targeting human METTL3 #1

    Depositor

    Mark Rubin

    Article

    Cotter et al Mol Cancer Res. 2021 Jun 4. pii: 1541-77

    Insert

    methyltransferase like 3 (METTL3 Human)
    

    Use

    Lentiviral

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBS31-cCARLIN

    Plasmid

    #160928

    Purpose

    Contains 10 constitutively expressed guide RNAs and CAG-GFP-CARLIN array sequence for DNA barcoding (when used in combination with Cas9)

    Depositor

    Fernando Camargo

    Article

    Bowling et al Cell. 2020 Jun 11;181(6):1410-1422.e27.

    Insert

    CARLIN gRNAs and CAG-GFP-CARLIN array
    

    Use

    Mouse Targeting

    Expression

    Mammalian

    Promoter

    U6 promoter for gRNAs; CAG promoter for GFP

    Available Since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. MSCV-Tox-IRES-eGFP

    Plasmid

    #165427

    Purpose

    Expresses TOX in mammalian cells

    Depositor

    Patrick Hogan, Anjana Rao

    Article

    Seo et al Proc Natl Acad Sci U S A. 2019 Jun 18;11

    Insert

    TOX (Tox Mouse)
    

    Tags

    3xflag 5' of Tox

    Expression

    Mammalian

    Promoter

    5' LTR

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pL

    Plasmid

    #196286

    Purpose

    35S promoter-driven expression of the positive-sense TSWV L RNA segment encoding codon-optimized L (RdRp)

    Depositor

    Zhenghe Li

    Article

    Liu et al Mol Plant. 2023 Mar 6;16(3):616-631. doi

    Insert

    Full length TSWV L antigenome encoding codon-optimized L (RdRp)
    

    Use

    CRISPR

    Expression

    Plant

    Promoter

    duplicated cauliflower mosaic virus 35S promoter

    Available Since

    April 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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