162,111 results

1. pQCXIZ GFP PODXL 5N>Q

   Plasmid

   #202419

   Purpose

   Expression of GFP-tagged PODXL with mutated N-linked glycosylation sites

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

   Insert

   PODXL (PODXL Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mutation

   N33/43/104/144/360Q

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG RABV-G

   Plasmid

   #36398

   Depositor

   Connie Cepko

   Article

   Beier et al Proc Natl Acad Sci U S A. 2011 Sep 13;10

   Insert

   rabies virus glycoprotein
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   CAGGs

   Available since

   Aug. 3, 2012

   Availability

   Academic Institutions and Nonprofits only

3. pLenti-CMV-GSDMD-eGFP-SV40p-BlasR

   Plasmid

   #228254

   Purpose

   pLenti-CMV-GSDMD-eGFP-SV40p-BlasR plasmid for ectopic expression of human full-length GSDMD fusion with GFP

   Depositor

   Judy Lieberman

   Article

   Fontana et al Cell. 2024 Sep 2:S0092-8674(24)00898-5.

   Insert

   GSDMD (GSDMD Human)
   

   Use

   Lentiviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEThT-RvLEAM

   Plasmid

   #90033

   Purpose

   To express His-tagged RvLEAM in bacteria

   Depositor

   Takekazu Kunieda

   Article

   Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

   Insert

   RvLEAM
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBad-sfGFP 150TAG

   Plasmid

   #85483

   Purpose

   Arabinose inducible E. coli codon optimized superfolder GFP with TAG stop codon at position 150

   Depositor

   Ryan Mehl

   Article

   Miyake-Stoner et al Biochemistry. 2010 Mar 2;49(8):1667-77.

   Insert

   superfolder GFP 150TAG
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   150TAG (amino acid 150 mutated to stop codon)

   Promoter

   Arabinose

   Available since

   Jan. 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. CAPN1

   Plasmid

   #25607

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   CAPN1 (CAPN1 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Contains amino acids 29-360

   Promoter

   Available since

   Oct. 25, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

7. pLenti UBC LC3Flux (HaloTag7-mGFP-LC3-STOP)

   Plasmid

   #215014

   Purpose

   Express an autophagy (LC3) reporter in mammalian cells (untested)

   Depositor

   Wade Harper

   Insert

   MAP1LC3B (Map1lc3b Rat)
   

   Use

   Lentiviral

   Tags

   HaloTag(7)-mGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. 5aa SunTag VP64 EVD

   Plasmid

   #115483

   Purpose

   CRISPR-Cas9 SunTag system to target VP64 to the EVD/ATR loci each with two guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Papikian et al Nat Commun. 2019 Feb 13;10(1):729. doi:

   Insert

   g2_U6_g1_U6_NOS_NLS_GB1_NLS_linker_VP64_linker_sfGFP_scFv_UBQ10_Insulator_UBQ10_Ω_dCas9_1xHA_2xNLS_linker_10xGCN4_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLenti.CMV/TO_PRKAA1

   Plasmid

   #74446

   Purpose

   Lentiviral expression vector encoding untagged WT human AMPK alpha 1

   Depositor

   Reuben Shaw

   Article

   Toyama et al Science. 2016 Jan 15;351(6270):275-81. d

   Insert

   AMPK alpha 1 (PRKAA1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 27, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLY100-RetroEFS-HER2-28BBz-WPRE

    Plasmid

    #192201

    Purpose

    Retro-HER2CAR

    Depositor

    Sidi Chen

    Article

    Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

    Insert

    Retro-HER2CAR (ERBB2 Synthetic)
    

    Use

    Retroviral; Mammalian expression

    Tags

    Expression

    Mutation

    NA

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. TFORF2049

    Plasmid

    #143866

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    PURA (PURA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP VAMP2

    Plasmid

    #42308

    Depositor

    Thierry Galli

    Article

    Martinez-Arca et al Proc Natl Acad Sci U S A. 2003 Jul 22;10

    Insert

    VAMP 2
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLEX_305

    Plasmid

    #41390

    Purpose

    Constitutive lentiviral expression, SV40-puro; PGK-gateway-no tag

    Depositor

    David Root

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available since

    Nov. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pSL2680

    Plasmid

    #85581

    Purpose

    Replicating base vector for constructing cpf1/CRISPR editing plasmids

    Depositor

    Himadri Pakrasi

    Article

    Ungerer et al Sci Rep. 2016 Dec 21;6:39681. doi: 10.10

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pBiFC-bJunVN173

    Plasmid

    #22012

    Depositor

    Chang-Deng Hu

    Article

    Shyu et al Biotechniques. 2006 Jan . 40(1):61-6.

    Insert

    bJunVN173 (Jun Aequorea Victoria, Rat)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    c-Jun(257-338)-Venus(1-172)

    Promoter

    Available since

    Sept. 28, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-DIO-EF1α-mKate2-biPAC

    Plasmid

    #169127

    Purpose

    AAV plasmid containing a mKate2 and a light-gated adenylate cyclase biPAC

    Depositor

    Georg Nagel

    Article

    Zhang et al Nature. 2021 Sep;597(7875):245-249. doi:

    Insert

    mKate2-biPAC
    

    Use

    AAV and Cre/Lox

    Tags

    mKate2

    Expression

    Mammalian

    Mutation

    codon optimized to mouse

    Promoter

    EF1a

    Available since

    Jan. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. LentiV2-mU6-evopreQ1

    Plasmid

    #210190

    Purpose

    pegRNA/ngRNA library cloning

    Depositor

    Yin Shen

    Article

    Ren et al Mol Cell. 2023 Dec 21;83(24):4633-4645.e

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. LeGO-C2

    Plasmid

    #27339

    Depositor

    Boris Fehse

    Article

    Weber et al Mol Ther. 2008 Apr . 16(4):698-706.

    Insert

    SFFV promoter, mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 24, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. HDM_VSV_G

    Plasmid

    #204156

    Purpose

    VSV-G expression plasmid

    Depositor

    Jesse Bloom

    Article

    Dadonaite et al Cell. 2023 Mar 16;186(6):1263-1278.e20.

    Insert

    VSV-G
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. NW50

    Plasmid

    #133445

    Purpose

    Covalently circularized nanodisc NW50 for studying membrane proteins and viral entry.

    Depositor

    Gerhard Wagner

    Article

    Nasr et al Nat Methods. 2017 Jan;14(1):49-52. doi:

    Insert

    NW50
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge