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Flag-SKP1-T131A

Plasmid

#213482

Purpose

Expresses human SKP1 T131A mutant in mammalian cells

Depositor

Michele Pagano

Article

Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

Insert

human Flag tagged SKP1-T131A (SKP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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Flag-SKP1-T131E

Plasmid

#213601

Purpose

Expresses human SKP1 T131E mutant in mammalian cells

Depositor

Michele Pagano

Article

Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

Insert

human Flag tagged SKP1-T131E (SKP1 Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsAGO1_F

Plasmid

#146314

Purpose

Mammalian Expression of HsAGO1

Depositor

Elisa Izaurralde

Article

Lazzaretti et al RNA. 2009 Jun . 15(6):1059-66.

Insert

HsAGO1 (AGO1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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mTurquoise2-ShadowY

Plasmid

#193557

Purpose

Expresses mTurquoise2-ShadowY in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

mTurquoise2-ShadowY

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-MERTK-Fc(DAPA)-AviTag-6xHis

Plasmid

#156878

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

MERTK (MERTK Human)

Use

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available since

Jan. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pCK013

Plasmid

#105345

Purpose

Bacterial translocation fo proteins into plant cells

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

secretion signal, ccdB cassette

Use

Bacterial secretion

Tags

Expression

Mutation

BsaI restriction sites removed

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCK016

Plasmid

#105348

Purpose

Bacterial translocation for proteins into plant cells

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

secretion signal, ccdB cassette

Use

Bacterial secretion

Tags

Expression

Mutation

BsaI restriction sites removed

Promoter

Available since

Aug. 1, 2018

Availability

Academic Institutions and Nonprofits only

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pCK014

Plasmid

#105346

Purpose

Bacterial translocation fo proteins into plant cells

Depositor

Johannes Stuttmann

Article

Gantner et al PLoS One. 2018 May 30;13(5):e0197185. do

Insert

secretion signal, ccdB cassette

Use

Bacterial secretion

Tags

Expression

Mutation

BsaI restriction sites removed

Promoter

Available since

May 10, 2018

Availability

Academic Institutions and Nonprofits only

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pBabepuroBAF180

Plasmid

#41078

Depositor

Ramon Parsons

Article

Xia et al Cancer Res. 2008 Mar 15;68(6):1667-74.

Insert

BAF180 (PBRM1 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

3 silent mutations: 3595 a to g; 4981 a to t; 587…

Promoter

Available since

Jan. 31, 2013

Availability

Academic Institutions and Nonprofits only

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pLEX307 RFP

Plasmid

#163976

Purpose

Mammalian expression vector encoding RFP (for example, to normalize plasmid-based protein expression)

Depositor

Daniel Bachovchin

Article

Robert Hollingsworth et al Nat Commun. 2021 Jan 8;12(1):189. doi: 1

Insert

RFP

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pEB-C5

Plasmid

#28213

Purpose

5 reprogramming factors (OCT4, SOX2, KLF4, c-MYC and LIN28) are expressed as a single poly-cistronic unit

Depositor

Linzhao Cheng

Article

Chou et al Cell Res. 2011 Jan 18. ():.

Insert

Oct4, Sox2, Klf4, c-Myc, Lin28 (Myc, Sox2, Pou5f1, Klf4, Lin28a Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 20, 2011

Availability

Academic Institutions and Nonprofits only

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pX330 _CRISPIE donor B7

Plasmid

#172848

Purpose

DRS-2 sgRNA expression under a U6 promotor and CRISPIE donor B7 (Zhong et al, eLife 2021), mEGFP translational phase (0-0), excised by DRS-1 or DRS-2 sgRNA (with SpCas9).

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

DRS-2 sgRNA and CRISPIE designer exon (phase 0-0) encoding mEGFP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-Actin-7

Plasmid

#56421

Purpose

Localization: Actin, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Actin (ACTB Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 23, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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TFORF1344

Plasmid

#143792

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

FEZF2 (FEZF2 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

June 8, 2023

Availability

Academic Institutions and Nonprofits only

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pS448∙CsR

Plasmid

#122096

Purpose

CRISPR/Cas9 counterselection in Gram-negative bacteria

Depositor

Pablo Ivan Nikel

Article

Wirth et al Microb Biotechnol. 2019 Mar 12. doi: 10.

Inserts

Cas9
sgRNA

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Pem7 and Pm

Available since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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pMK286 (mAID-NeoR)

Plasmid

#72824

Purpose

C-terminal tagging with mAID using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-Neo

Use

CRISPR

Tags

mAID

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA-HDAC6-FLAG

Plasmid

#30482

Depositor

Tso-Pang Yao

Article

Kawaguchi et al Cell. 2003 Dec 12. 115(6):727-38.

Insert

HDAC6 (HDAC6 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 6, 2011

Availability

Academic Institutions and Nonprofits only

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FUW-Luc-mCherry-puro

Plasmid

#183685

Purpose

Lentiviral vector expressing luciferase and mCherry.

Depositor

Andrew Kung

Article

Kimbrel et al Mol Imaging. 2009 May-Jun;8(3):140-7.

Insert

Luc-E2A-mCherry-T2A-PuroR

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Human Ubiquitin C (UbC) promoter

Available since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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PB-CA sfGFP

Plasmid

#159094

Purpose

PB-CA sfGFP piggyBac transposon

Depositor

Mario Capecchi

Insert

sfGFP

Use

Piggybac

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 6, 2020

Availability

Academic Institutions and Nonprofits only

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mEGFP-HRas G12V

Plasmid

#18666

Depositor

Karel Svoboda

Article

Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

Insert

H-Ras (HRAS Human)

Use

Tags

monomeric EGFP

Expression

Mammalian

Mutation

G12V mutation in HRas

Promoter

Available since

July 2, 2008

Availability

Academic Institutions and Nonprofits only

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