164,577 results

1. pT2-shP53

   Plasmid

   #124261

   Purpose

   Expresses shRNA targeting P53. Construct has inverted repeats to be used in Sleeping beauty system.

   Depositor

   Maria Castro

   Article

   Nunez et al Sci Transl Med. 2019 Feb 13;11(479). pii

   Insert

   shP53
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 8, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. c-myc promoter (Del 4)

   Plasmid

   #16604

   Depositor

   Bert Vogelstein

   Article

   He et al Science. 1998 Sep 4. 281(5382):1509-12.

   Insert

   c-myc promoter (Del 4) (MYC Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Del-4 (PvuII->PvuII). The first PvuII site is …

   Promoter

   Available Since

   March 24, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCW-Tet-Cas9-BFP-PGK-Blast

   Plasmid

   #196720

   Purpose

   Tet-inducible expression of Cas9-T2A-TagBFP

   Depositor

   Bernhard Schmierer

   Insert

   Cas9-T2A-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   3xFLAG-SV40NLS and Nucleoplasmin NLS

   Expression

   Mutation

   Promoter

   TRE, PGK

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMV-SEAP

   Plasmid

   #24595

   Depositor

   Alan Cochrane

   Insert

   SEAP (ALPP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 23, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV hSYN DIO mGluR1-myc-FLAG

   Plasmid

   #166227

   Purpose

   AAV expressing cre-dependent mGluR1-myc-FLAG

   Depositor

   Jason Aoto

   Article

   Cai et al Neuron. 2021 Apr 7;109(7):1137-1149.e5.

   Insert

   metabotropic glutamate receptor 1 (Grm1 Mouse)
   

   Use

   AAV and Cre/Lox

   Tags

   Myc and FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   human Synapsin

   Available Since

   April 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTBNde(min)

   Plasmid

   #15125

   Depositor

   Franco Pagani

   Article

   Pagani et al Hum Mol Genet. 2003 May 15. 12(10):1111-

   Insert

   alpha-globin EDB fibronectin minigene (FN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   alpha-globin

   Available Since

   March 5, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pST_140_LVL2 cam

   Plasmid

   #179334

   Purpose

   NT-CRISPR plasmid for a single gRNA with spG Cas9 (near PAM-less Cas9 variant).

   Depositor

   Anke Becker

   Article

   Stukenberg et al Commun Biol. 2022 Mar 25;5(1):265. doi:

   Insert

   Ptac tfoX, Ptet spG cas9, PJ23106 acrIIA4, Ptet gRNA with sfGFP dropout
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Cas9 -> SpG Cas9 (D1135L, S1136W, G1218K, E121…

   Promoter

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCMV-Hs.cargo(Cas9)

   Plasmid

   #174864

   Purpose

   Expresses human Peg10 cargoRNA encoding SpCas9

   Depositor

   Feng Zhang

   Article

   Segel et al Science. 2021 Aug 20;373(6557):882-889.

   Insert

   SpCas9 flanked by HsPeg10 UTRs
   

   Use

   Tags

   HA-SV40 NLS and SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. LV-TRE-VP64 human MyoD-T2A-dsRedExpress2

   Plasmid

   #60629

   Purpose

   Expresses VP64 human MyoD and dsRedExpress2 in response to doxycycline

   Depositor

   Charles Gersbach

   Article

   Kabadi et al ACS Synth Biol. 2014 Dec 10.

   Insert

   VP64 human MyoD (MYOD1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   TRE

   Available Since

   Dec. 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. PresentER-Cassette mCherry

    Plasmid

    #102943

    Purpose

    PresentER with removable cassette for cloning of MHC-I antigen (mCherry version)

    Depositor

    David Scheinberg

    Article

    Gejman et al Elife. 2018 Nov 30;7. pii: 41090. doi: 1

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLVX-TetOne-Puro-MCL1

    Plasmid

    #202663

    Purpose

    Tet-ON 3rd generation expression vector with inducible expression of MCL1 and a puromycin selection cassette

    Depositor

    Jason Sheltzer

    Article

    Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

    Insert

    MCL1 apoptosis regulator, BCL2 family member (MCL1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV-GFAP-hM3D(Gq)-mCherry

    Plasmid

    #50478

    Purpose

    Gq-coupled hM3D DREADD fused with mCherry under the control of GFAP promoter

    Depositor

    Bryan Roth

    Has Service

    AAV5

    Insert

    hM3D(Gq)-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    GFAP

    Available Since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET302-6His-dCas9-Halo

    Plasmid

    #72269

    Purpose

    Encodes a Halo-labeled fusion of nuclease deficient Cas9

    Depositor

    Timothée Lionnet

    Article

    Deng et al Proc Natl Acad Sci U S A. 2015 Sep 22;11

    Insert

    His6-dCas9-Halo
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFUSEss-CHIg-mG1_M18

    Plasmid

    #82357

    Purpose

    Expression plasmid coding for heavy chain of mouse IgG1 antibody specific to antigen B of the ABO blood group system, clone M18.

    Depositor

    Joanna Bereta

    Article

    Klaus et al Sci Rep. 2016 Aug 3;6:30938. doi: 10.103

    Insert

    mouse immunoglobulin heavy chain IgG1 isotype (Ighg1 Mouse)
    

    Use

    Tags

    none

    Expression

    Mammalian

    Mutation

    none

    Promoter

    hEF1-HTLV prom

    Available Since

    Sept. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCIN BRCA2 WT

    Plasmid

    #16245

    Depositor

    Mien-Chie Hung

    Article

    Wang et al Cancer Res. 2002 Mar 1. 62(5):1311-4.

    Insert

    BRCA2 (BRCA2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSL21-VEX

    Plasmid

    #158230

    Purpose

    Retroviral expression plasmid of sgRNA with violet-excited fluorescent protein (VEX)

    Depositor

    Junwei Shi

    Article

    Chen et al Cell. 2021 Mar 4;184(5):1262-1280.e22. d

    Type

    Empty backbone

    Use

    CRISPR and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 promoter for crRNA expression and EFS promoter…

    Available Since

    Jan. 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. M13KO7-ΔPS-ΔgeneIII

    Plasmid

    #134351

    Purpose

    Helper phage for M13 phage production

    Depositor

    Mark Isalan

    Article

    Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

    Insert

    Helper phage gene
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    June 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pEGFP-BLM

    Plasmid

    #110299

    Purpose

    Mammalian expression of a EGFP-tagged full length Bloom's syndrome helicase

    Depositor

    Chris Kok-Lung Chan

    Insert

    BLM (Bloom's Syndrome) helicase (BLM Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+) Flag-His-ATM kd

    Plasmid

    #31986

    Depositor

    Michael Kastan

    Article

    Canman et al Science. 1998 Sep 11;281(5383):1677-9.

    Insert

    ATM kd (ATM Human)
    

    Use

    Tags

    Flag and His

    Expression

    Mammalian

    Mutation

    3'UTRf 9 nucl/D2870A N2875k. catalytically …

    Promoter

    Available Since

    March 12, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMSCV-mCherry-Syk

    Plasmid

    #50045

    Purpose

    Expression of mCherry-Syk in mammalian cells

    Depositor

    Hidde Ploegh

    Article

    Strijbis et al PLoS Pathog. 2013 Jun;9(6):e1003446. doi

    Insert

    Spleen tyrosine kinase (Syk) (Syk Mouse)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    pCMV

    Available Since

    Jan. 13, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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