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pET-6xHis-GFP(-6)-H2_mCherry(-6)

Plasmid

#205049

Purpose

Co-expressed fluorescent proteins with charge-patterned cationic peptide to promote multi-protein biomolecular condensate formation in E. coli

Depositor

Allie Obermeyer

Article

Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

Insert

GFP(-6)-H2_mCherry(-6)

Tags

MGHHHHHGGA

Expression

Bacterial

Available Since

Feb. 9, 2024

Availability

Academic Institutions and Nonprofits only

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Cavbeta1 Ca2+ channel scFv [N7/18]

Plasmid

#206789

Purpose

Mammalian Expression of Cavbeta1 Ca2+ channel scFV. Derived from hybridoma N7/18 scFv.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Cavbeta1 Ca2+ channel (Homo sapiens) recombinant scFV (CACNB1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2023

Availability

Academic Institutions and Nonprofits only

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MultiMate-HITI-2c ACTB

Plasmid

#206267

Purpose

All in one recombinant baculovirus production vector. Encodes Cas9, sgRNA and donor for homology independent targeted integration in the ACTB locus.

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

SpCas9 (S. Pyogenes), hACTB HITI-2c donor, mCherry, Puro-R, hU6 hACTB sgRNA, AcrIIA4, eGFP

Use

CRISPR; Recombinant baculovirus production

Expression

Mammalian

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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MultiMate-PE3 HEK3

Plasmid

#206273

Purpose

Vector for the production of recombinant baculovirus using MultiMate assembly. All-in-one vector for PE3 HEK3 prime editing.

Depositor

Imre Berger

Article

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

PE2 (synthetic), hU6 HEK3 PegRNA, hU6 HEK3 sgRNA, aeBlue (E. quadricolor), VSV-G, TagBFP

Use

CRISPR; Recombinant baculovirus production

Expression

Mammalian

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-Dm4EHP-W114A_AH

Plasmid

#148909

Purpose

Insect Expression of Dm4EHP-W114A

Depositor

Elisa Izaurralde

Article

Ruscica et al Nucleic Acids Res. 2019 Jul 26;47(13):70

Insert

Dm4EHP-W114A (4EHP Fly)

Expression

Insect

Mutation

4 silent mutations compared to the sequence given…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-SIGLEC1(Trunc2)-COMP5AP-AviTag-9xHis

Plasmid

#157563

Purpose

Mammalian expression of cell-surface protein partial extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SIGLEC1 (SIGLEC1 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-CD22-COMP5AP-AviTag-9xHis

Plasmid

#157504

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

CD22 (CD22 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-SIGLEC10-COMP5AP-AviTag-9xHis

Plasmid

#157470

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SIGLEC10 (SIGLEC10 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-SIGLEC14-COMP5AP-AviTag-9xHis

Plasmid

#157345

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SIGLEC14 (SIGLEC14 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-SIGLEC6-COMP5AP-AviTag-9xHis

Plasmid

#157326

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SIGLEC6 (SIGLEC6 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-SIGLEC9-COMP5AP-AviTag-9xHis

Plasmid

#157335

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to COMP5AP-AviTag-9xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

SIGLEC9 (SIGLEC9 Human)

Tags

COMP5AP-AviTag-9xHis

Expression

Mammalian

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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pD649-HAsp-FCMR-Fc(DAPA)-AviTag-6xHis

Plasmid

#156684

Purpose

Mammalian expression of cell-surface protein extracellular domain fused to Fc(DAPA)-Avi-6xHis. Protein is secreted from cells.

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

FCMR (FCMR Human)

Tags

Fc(DAPA)-AviTag-6xHis

Expression

Mammalian

Mutation

Fc region of human IgG contains D265A and P329A m…

Promoter

CMV/SP6

Available Since

March 8, 2023

Availability

Academic Institutions and Nonprofits only

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PRPF4.001.169

Plasmid

#137233

Purpose

Expresses N-terminal (His)6-TEV cleavage site fusion of human gene or portion of gene in bacterial strains. pET based vector.

Depositor

Tara Davis

Article

Rajiv et al Biochem J. 2017 Oct 25;474(21):3689-3704

Insert

PRPF4 (PRPF4 Human)

Tags

(His)6-TEV

Expression

Bacterial

Mutation

001-169

Available Since

Nov. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pG.Lantern-INS-SM50-GFP

Plasmid

#135594

Purpose

Propogation of Insulator-SM50 Promoter-GFP DNA for microinjection to visualize Insulator-GFP promoter activity in S. purpuratus

Depositor

Koji Akasaka, Mamiko Yajima

Article

Yajima et al Dev Genes Evol. 2007 Apr;217(4):331-6. d

Insert

INS-SM50 promoter (SM50 )

Use

In vitro transcription (mrna synthesis)

Tags

Green Lantern GFP

Promoter

CMV

Available Since

Jan. 7, 2022

Availability

Academic Institutions and Nonprofits only

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p166

Plasmid

#186180

Purpose

Shotgun Metagenomic Spike-in

Depositor

Karsten Zengler

Article

Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

Insert

synDNA 166

Use

Synthetic Biology; Cloning

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pPD0039_CROP-seq-CAR-Puro

Plasmid

#242532

Purpose

CAR T screening

Depositor

Christoph Bock, Paul Datlinger

Article

Datlinger et al Nature. 2025 Oct;646(8086):963-972. doi:

Insert

CD19-BB CAR (CD19 Human)

Use

Lentiviral

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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ER-RpHLuorin2

Plasmid

#171717

Purpose

Luminal ER expression of ratiometric pHLuorin2

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2 targeted to ER

Tags

KDEL ER-retention signal and Signal sequence of h…

Expression

Mammalian

Promoter

CMV

Available Since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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HIV-1-GFP ΔEnv

Plasmid

#217437

Purpose

HIV-1 reporter vector that is env-deficient and expresses eGFP in place of nef; contains 5' and 3' LTRs, gag, pol, tat, and rev, but does not express vif, vpr, or vpu.

Depositor

Jarrod Johnson

Article

Scott et al PLoS Pathog. 2025 Jan 28;21(1):e1012206.

Insert

gag/pol

Use

Lentiviral

Available Since

Jan. 28, 2025

Availability

Academic Institutions and Nonprofits only

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p256

Plasmid

#186184

Purpose

Shotgun Metagenomic Spike-in

Depositor

Karsten Zengler

Article

Zaramela et al mSystems. 2022 Dec 20;7(6):e0044722. doi

Insert

synDNA 256

Use

Synthetic Biology; Cloning

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-Myo10

Plasmid

#135403

Purpose

Expresses GFP-tagged bovine Myo10 in mammalian cells.

Depositor

Richard Cheney

Article

Berg et al Nat Cell Biol. 2002 Mar;4(3):246-50. doi

Insert

Myo10 (MYO10 Bovine)

Tags

EGFP

Expression

Mammalian

Mutation

"Relative to the GenBank Myo10 cDNA sequence…

Promoter

CMV

Available Since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

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