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Showing: 9621 - 9640 of 158069 results

  1. pDEST17-His10-ccdB

    Plasmid
    #166145
    Purpose
    Gateway destination vector for generation of N-terminal His10-tagged proteins in E. coli.
    Depositor
    Daniel Jarosz
    Article
    Itamar Harel et al BioRxiv
    Type
    Empty backbone
    Use
    Tags
    His10
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 20, 2024
    Availability
    Academic Institutions and Nonprofits only
    166145-plasmid-map-sequence-id-328877

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  4. phCMV-229E-D15

    Plasmid
    #188908
    Purpose
    express truncated 229E spike for pseudovirus production
    Depositor
    David Nemazee
    Insert
    229E spike (S 229E)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    c-terminal 15aa deletion
    Promoter
    CMV
    Available since
    Aug. 30, 2022
    Availability
    Academic Institutions and Nonprofits only
    188908-plasmid-map-sequence-id-370665

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  14. pCMV-T7-SpG-HF1-P2A-EGFP (RTW5000)

    Plasmid
    #139996
    Purpose
    CMV and T7 promoter expression plasmid for human codon optimized SpG-HF1(SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R;HF1=N497A/R661A/Q695A/Q926A) with a c-terminal bi-partite NLS, 3x flag tag, and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized SpCas9 variant named SpG-HF1 with BPNLS-3xFLAG-P2A-EGFP
    Use
    CRISPR; In vitro transcription; t7 promoter
    Tags
    BPNLS-3xFLAG-P2A-EGFP
    Expression
    Mammalian
    Mutation
    SpG=D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R; HF…
    Promoter
    CMV and T7
    Available since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    139996-plasmid-map-sequence-id-275405

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Showing: 9621 - 9640 of 158069 results