162,109 results

1. LentiGuide-TagBFP-2A-BSD

   Plasmid

   #167929

   Purpose

   Lentiviral gRNA expression with TagBFP fluorescence and blasticidin selection marker.

   Depositor

   Ronald Germain

   Article

   Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6, EF1a

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAD-LyseR

   Plasmid

   #99244

   Purpose

   For autolysate production in E. coli BL21-Gold (DE3) strain: constitutively expresses phage lambda endolysin (gene R)

   Depositor

   Jeff Hasty

   Article

   Didovyk et al ACS Synth Biol. 2017 Aug 10. doi: 10.102

   Insert

   phage lambda gene R (R phage lambda)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   beta-lactamase promoter

   Available since

   Oct. 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBOB-Lck-GFP

   Plasmid

   #118738

   Purpose

   To express membrane targeted GFP

   Depositor

   John Cooper

   Insert

   Lck domain (LCK Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mutation

   H8N (see comments)

   Promoter

   CMV

   Available since

   Nov. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEGFP-Aurora A

   Plasmid

   #198402

   Purpose

   Expression of EGFP-tagged Aurora A in mammalian cells

   Depositor

   Kenji Sugimoto

   Article

   Sugimoto et al Cell Struct Funct. 2002 Dec;27(6):457-67

   Insert

   Aurora A
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGal4AD-CIB1

   Plasmid

   #28245

   Purpose

   Expresses Gal4 Activation Domain fused to CIB1 for blue-light-induced transcription activation

   Depositor

   Chandra Tucker

   Article

   Kennedy et al Nat Methods. 2010 Dec;7(12):973-5. doi:

   Insert

   Gal4AD (CIB1, GAL4 Budding Yeast)
   

   Use

   Tags

   CIB1

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   March 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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6. GCEPIA1-SNAPER

   Plasmid

   #172659

   Purpose

   Detect the real-time ER calcium store and local calcium microdomains in live cells.

   Depositor

   Pingyong Xu

   Article

   Luo et al Biophys Rep (2019) 5, 31–42

   Insert

   calmodulin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Please see depositor comments

   Promoter

   Available since

   Aug. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEx-4T-1-YTHDF3

   Plasmid

   #70088

   Purpose

   Encode YTHDF3 isoform a with N-terminal GST tag for E. coli expression

   Depositor

   Chuan He

   Article

   Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

   Insert

   YTH N(6)-methyladenosine RNA binding protein 3 (YTHDF3 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pLEX_305

   Plasmid

   #41390

   Purpose

   Constitutive lentiviral expression, SV40-puro; PGK-gateway-no tag

   Depositor

   David Root

   Type

   Empty backbone

   Use

   Lentiviral; Gateway destination vector

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Nov. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLY100-RetroEFS-HER2-28BBz-WPRE

   Plasmid

   #192201

   Purpose

   Retro-HER2CAR

   Depositor

   Sidi Chen

   Article

   Ye et al Cell Metab. 2022 Apr 5;34(4):595-614.e14

   Insert

   Retro-HER2CAR (ERBB2 Synthetic)
   

   Use

   Retroviral; Mammalian expression

   Tags

   Expression

   Mutation

   NA

   Promoter

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCherry-Rab4a-7

    Plasmid

    #55125

    Purpose

    Localization: Ras GTPase, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Rab4a (RAB4A Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti6.2_3xFLAG_V5_ eGFP_V5

    Plasmid

    #160111

    Purpose

    Expresses eGFP in mammalian cells

    Depositor

    Rizwan Haq

    Article

    Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

    Insert

    eGFP
    

    Use

    Lentiviral

    Tags

    3xFLAG, V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS-mTagBFP2-T2A-iCre (v2)

    Plasmid

    #125822

    Purpose

    Monomeric TagBFP2 and iCre recombinase (version 2)

    Depositor

    Mario Capecchi

    Insert

    mTagBFP2-T2A-iCre
    

    Use

    Cre/Lox

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pH-nCas9-PBE

    Plasmid

    #98163

    Purpose

    Expresses CRISPR base editor in rice

    Depositor

    Caixia Gao

    Article

    Zong et al Nat Biotechnol. 2017 May;35(5):438-440.

    Insert

    NLS-APOBEC1-XTEN-nCas9-UGI-NLS
    

    Use

    Rice

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Ubi

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. GFP-APEX2-NIK3x

    Plasmid

    #129274

    Purpose

    Expression of APEX2 in the nucleolus

    Depositor

    Alice Ting

    Article

    Fazal et al Cell. 2019 Jun 14. pii: S0092-8674(19)30

    Insert

    GFP-APEX2-NIK3x
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    Aug. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMSCVpuro-tdTomato

    Plasmid

    #97079

    Purpose

    Retroviral plasmid containing tdTomato for labeling of cells

    Depositor

    Lee Grimes

    Article

    Kleeman et al Cytometry A. 2018 Mar 13. doi: 10.1002/c

    Insert

    tdTomato
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAG-D1R

    Plasmid

    #89160

    Purpose

    Express vaccinia virus D1R in mammalian cells

    Depositor

    Takeshi Kobayashi

    Article

    Kanai et al Proc Natl Acad Sci U S A. 2017 Jan 30. p

    Insert

    Vaccinia virus D1R
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG promotor

    Available since

    April 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEThT-RvLEAM

    Plasmid

    #90033

    Purpose

    To express His-tagged RvLEAM in bacteria

    Depositor

    Takekazu Kunieda

    Article

    Tanaka et al PLoS One. 2015 Feb 12;10(2):e0118272. eC

    Insert

    RvLEAM
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDB5318

    Plasmid

    #204828

    Purpose

    An integrating plasmid containing both the inducible enotetS promoter and the coding sequence of the TetR repressor under the control of the constitutive CMV promoter.

    Depositor

    Li-Lin Du

    Article

    Lyu et al J Cell Sci. 2024 Nov 1;137(21):jcs263404

    Inserts

    tetR
    mECitrine
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CMV promoter and enotetS promoter

    Available since

    Aug. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pU6-gRNA

    Plasmid

    #53062

    Purpose

    Transcription of guide RNA (gRNA) in wheat cells

    Depositor

    Caixia Gao

    Article

    Shan et al Nat Biotechnol. 2013 Aug;31(8):686-8. do

    Insert

    wheat U6 promoter - gRNA
    

    Use

    CRISPR; Plant expression

    Tags

    Expression

    Mutation

    Promoter

    wheat U6

    Available since

    Aug. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. pcDNA3/Myc-DNMT3B1

    Plasmid

    #35522

    Purpose

    Mammalian expression of human DNMT3B1 with myc tag

    Depositor

    Arthur Riggs

    Article

    Chen et al J Cell Biochem. 2005 Aug 1;95(5):902-17.

    Insert

    DNMT3B1 (DNMT3B Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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