166,276 results

1. CVS-N2c-nl.EGFP-jRGECO1a

   Plasmid

   #172386

   Purpose

   Production of RVdG-CVS-N2c viral vectors for cell-type specific trans-synaptic retrograde labeling.

   Depositor

   Yoav Ben-Simon, Peter Jonas

   Article

   Sumser et al Elife. 2022 Aug 30;11:e79848. doi: 10.75

   Insert

   nuclear-localized EGFP + jRGECO1a
   

   Use

   Neurotropic virus

   Available Since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pMuLE_EXPR_CMV-eGFP_TOP-iRFP_PTCH1-mTurquoise2_CBF-tdTomato

   Plasmid

   #113865

   Purpose

   Triple pathway reporter, 3P-Fluor; wnt-iRFP, hedgehog-mTurquoise2, notch-tdTomato; plus CMV-eGFP. Gateway expression vector for lentivirus generation.

   Depositor

   Manfred Ogris

   Article

   Maier et al PLoS One. 2019 Dec 20;14(12):e0226570. d

   Inserts

   CMV-eGFP
   TOP-iRFP
   PTCH1-mTurquoise2
   CBF-tdTomato
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. tdTomato-Lysosomes-20

   Plasmid

   #58109

   Purpose

   Localization: Lysosome Membrane, Excitation: 554, Emission: 581

   Depositor

   Michael Davidson

   Insert

   LAMP1
   

   Tags

   tdTomato

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 19, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pSLQ-mgU2-47

   Plasmid

   #127585

   Purpose

   Expresses mgU2-47 scaRNA from a mU6 promoter

   Depositor

   David Rueda

   Article

   Autour et al Nat Commun. 2018 Feb 13;9(1):656. doi: 1

   Insert

   mgU2-47 scaRNA
   

   Expression

   Mammalian

   Promoter

   modified U6

   Available Since

   July 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pAGM37443

   Plasmid

   #153218

   Purpose

   Level 2 binary vector for plant transformation; for cloning Cas9 and guide RNAs; single copy in Agrobacterium.

   Depositor

   Sylvestre Marillonnet

   Article

   Grutzner et al Plant Commun. 2020 Nov 23;2(2):100135. d

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   Aug. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. SPLICS Po-ER Short P2A

   Plasmid

   #164114

   Purpose

   Detect the long Peroxisomes-Endoplasmic reticulum contact

   Depositor

   Marisa Brini, Tito Calì

   Article

   Vallese et al Nat Commun. 2020 Nov 27;11(1):6069. doi:

   Insert

   Split YFP Po-ER Short P2A
   

   Expression

   Mammalian

   Available Since

   Feb. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pCMV5B-TGFbeta receptor II wt (N-term HA)

   Plasmid

   #24801

   Depositor

   Jeff Wrana

   Insert

   TGFbeta receptor II (TGFBR2 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   May 14, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pcDNA5FRT/Flag-KIAA1267

   Plasmid

   #29758

   Depositor

   Joan Conaway

   Article

   Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

   Insert

   KIAA1267 (KANSL1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Jan. 30, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET His10 TEV LIC cloning vector (2B-T-10)

   Plasmid

   #78173

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Tags

   10xHis and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 18, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. ssGNb-mCherry

    Plasmid

    #128788

    Purpose

    Mammalian expression

    Depositor

    Colin Taylor

    Article

    Prole et al BMC Biol. 2019 May 23;17(1):41. doi: 10.

    Insert

    ssGNb-mCherry
    

    Expression

    Mammalian

    Available Since

    Feb. 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pETDuet-LahT150

    Plasmid

    #166785

    Purpose

    Cleavage of double glycine motif in peptides to remove the leader sequence

    Depositor

    Wilfred van der Donk

    Article

    Bobeica et al Elife. 2019 Jan 14;8. pii: 42305. doi: 1

    Insert

    LahT150
    

    Tags

    His6

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. mCh-Sec61 beta

    Plasmid

    #49155

    Purpose

    expression of Sec61 beta fused to mCherry, used as a general ER marker

    Depositor

    Gia Voeltz

    Article

    Zurek et al Traffic. 2011 Jan;12(1):28-41. doi: 10.1

    Insert

    Sec61 beta (SEC61B Human)
    

    Tags

    mCherry*

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 20, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1-ccdB-3xFLAG

    Plasmid

    #214984

    Purpose

    Gateway compatible empty vector (transient mammalian expression) with a C-terminal 3xFLAG tag

    Depositor

    Wade Harper

    Type

    Empty backbone

    Tags

    3xFLAG

    Expression

    Mammalian

    Available Since

    March 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3-Casp8

    Plasmid

    #11817

    Depositor

    Guy Salvesen

    Article

    Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

    Insert

    Caspase 8 (CASP8 Human)
    

    Expression

    Mammalian

    Available Since

    May 25, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRS426 TEF mCherry

    Plasmid

    #129433

    Purpose

    express mcherry

    Depositor

    Oskar Laur

    Insert

    mcherry
    

    Expression

    Yeast

    Available Since

    March 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGL3 NSE promoter

    Plasmid

    #11606

    Depositor

    Rosalyn Adam

    Article

    Kim et al Endocrinology. 2004 Feb . 145(2):613-9.

    Insert

    NSE promoter (ENO2 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Available Since

    July 19, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV-syn-FLEX-jYCaMP1s

    Plasmid

    #135420

    Purpose

    Yellow protein calcium sensor expressed under human Synapsin1 promoter, Cre mediated flip-excision switch, for AAV production or direct transfection, slow variant

    Depositor

    Kaspar Podgorski

    Article

    Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

    Has Service

    AAV1

    Insert

    jYCaMP1s
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Jan. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAAV-CAG-eYFP

    Plasmid

    #104055

    Purpose

    An AAV genome that ubiquitously expresses the fluorescent protein eYFP from the CAG promoter

    Depositor

    Viviana Gradinaru

    Article

    Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV2, and AAV5

    Insert

    EYFP
    

    Use

    AAV

    Promoter

    CAG

    Available Since

    Jan. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAG-EGxxFP

    Plasmid

    #50716

    Purpose

    5’ and 3’ EGFP fragments that shares 482bp were placed under ubiquitous CAG promoter. Used for validation of gRNA sequences by DSB mediated EGFP reconstitution.

    Depositor

    Masahito Ikawa

    Article

    Mashiko et al Sci Rep. 2013 Nov 27;3:3355. doi: 10.103

    Insert

    split EGFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Feb. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pMEL12

    Plasmid

    #107918

    Purpose

    hph based Yeast/E.coli shuttle vector designed to clone and express Cas9 programming spacer

    Depositor

    Jean-Marc Daran

    Article

    Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov

    Insert

    gRNA-CAN1.Y
    

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge