159,557 results

1. Flag-HA-USP38

   Plasmid

   #22580

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   USP38 (USP38 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Promoter

   Available since

   Nov. 16, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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2. pBPhsFlp1

   Plasmid

   #32148

   Depositor

   Gerald Rubin

   Article

   Nern et al Proc Natl Acad Sci U S A. 2011 Aug 9.

   Insert

   hsFlp1
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Aspartic Acid at Amino Acid 5

   Promoter

   Hsp70Bb promoter

   Available since

   Sept. 23, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBiT3.1-N_STRAP:1-350

   Plasmid

   #211139

   Purpose

   Mammalian expression of full-length human STRAP. N-terminal HiBiT tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   STRAP:M1-A350 (STRAP Human)
   

   Use

   Tags

   MVSGWRLFKKISGSSGGSSG

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti_CMV:Flag-OgNLuc

   Plasmid

   #208851

   Purpose

   Expresses FLAG epitope-tagged OgNLuc "Orange" BRET reporter in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Labra et al iScience. 2024 Aug 8;27(9):110655. doi:

   Insert

   OgNLuc LumiFluor
   

   Use

   Lentiviral

   Tags

   1xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. mpx:UtrCH-GFP

   Plasmid

   #45247

   Depositor

   Anna Huttenlocher

   Article

   Yoo et al Dev Cell. 2010 Feb 16. 18(2):226-36.

   Insert

   utrophin (UTRN Human)
   

   Use

   Zebrafish expression

   Tags

   EGFP

   Expression

   Mutation

   contains amino acids 1-261 of NP_009055.2; Q236R

   Promoter

   mpx

   Available since

   Oct. 29, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMXS-IRES-Blast HA-POLE2

   Plasmid

   #160809

   Purpose

   Express HA-tagged POLE2

   Depositor

   Richard Possemato

   Article

   Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

   Insert

   HA-POLE2 (POLE2 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   Oct. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. Golgi-mEGFP

   Plasmid

   #182877

   Purpose

   Localization: Gal-T - Golgi Complex

   Depositor

   Salvatore Chiantia

   Article

   Petazzi et al J Virol. 2020 Nov 25. pii: JVI.01238-20.

   Insert

   Golgi-mEGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSCV-myc-CAR-2A-Thy1.1

   Plasmid

   #127890

   Purpose

   Expresses CART receptor along with tags.

   Depositor

   Anjana Rao

   Article

   Chen et al Nature. 2019 Mar;567(7749):530-534. doi:

   Inserts

   myc,
   CAR
   2A
   Thy1.1
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 8, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCB-SC101-Spe

   Plasmid

   #133765

   Purpose

   shuttle vector backbone

   Depositor

   Himadri Pakrasi

   Article

   Liu et al Microb Cell Fact. 2018 Mar 26;17(1):48.

   Type

   Empty backbone

   Use

   Synthetic Biology; Shuttle vector for e.coli and …

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUBQ:Citrine-TurboID-3xMyc

    Plasmid

    #199244

    Purpose

    This plasmid could be used to clone gene(protein) of interest in place of Citrine to use in proximity labeling approach in plants.

    Depositor

    Savithramma Dinesh-Kumar

    Article

    Zhang et al Nat Commun. 2019 Jul 19;10(1):3252. doi:

    Insert

    Citrine-TurboID-3xMyc
    

    Use

    Tags

    TurboID-3xMyc

    Expression

    Plant

    Mutation

    Promoter

    Arabidopsis UBQ10 promoter with intron

    Available since

    June 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTF102

    Plasmid

    #134774

    Purpose

    A binary plasmid for plant transformation containing _-glucuronidase gene driven by CaMV 35S promoter and a bialaphos resistance gene (bar) driven by CaMV 35S promoter and TEV enhancer.

    Depositor

    Kan Wang

    Article

    Frame et al Plant Physiol. 2002 May;129(1):13-22. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Nov. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV/hSyn-SCLM

    Plasmid

    #216760

    Purpose

    AAV2 transfer vector with the human synapsin I promoter for neuronal expression of SuperClomeleon

    Depositor

    George Augustine

    Insert

    SuperClomeleon
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    S30R and 11 AA deletion in the Cerulean CFP moiet…

    Promoter

    hSyn (human synapsin I)

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDG459

    Plasmid

    #100901

    Purpose

    SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.

    Depositor

    Paul Thomas

    Article

    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do

    Inserts

    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    

    Use

    CRISPR and Mouse Targeting

    Tags

    3xFLAG and T2A-PuroR

    Expression

    Mammalian

    Mutation

    Promoter

    CBh and U6

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET30a(+)-PE2

    Plasmid

    #180015

    Purpose

    Expressing PE2 protein in E.coli for protein purification

    Depositor

    Dirk Hockemeyer

    Article

    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

    Insert

    PE2
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Lac operator-controlled T7 promoter

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pmCherry-C1ab-Prkd1

    Plasmid

    #139314

    Purpose

    Diacylglycerol biosensor

    Depositor

    Gerry Hammond

    Article

    Zewe et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    mCherry:Mus musculus Prkd1(138-343) (Prkd1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    T208A, N313D

    Promoter

    Available since

    April 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAAV-hSynapsin1-FLEx-axon-jGCaMP8m-WPRE-SV40

    Plasmid

    #216533

    Purpose

    An axon-targeted jGCaMP8m controlled by hSynapsin1 promoter and flex switch

    Depositor

    Yuki Kambe

    Article

    Abe et al bioRxiv. 2023 Aug 24:2023.08.23.554475.

    Insert

    axon-jGCaMP8m
    

    Use

    AAV and Cre/Lox

    Tags

    GAP43

    Expression

    Mammalian

    Mutation

    Promoter

    hSynapsin1

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcdna3.1 hygro mCherry

    Plasmid

    #128057

    Purpose

    Mammalian expression vector with N terminal mCherry

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. hHS1

    Plasmid

    #159169

    Purpose

    Human codon-optimized cytosolic labile heme reporter

    Depositor

    Amit Reddi

    Article

    Hanna et al Proc Natl Acad Sci U S A. 2016 Jul 5;113

    Insert

    hHS1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pCFD5_w

    Plasmid

    #112645

    Purpose

    Ubiquitous expression of one or multiple sgRNAs in Drosophila. Selection marker: Mini-white.

    Depositor

    Michael Boutros

    Insert

    U6:3-tRNA-sgRNA expression cassette
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pFA6a-link-yomRuby2-Kan

    Plasmid

    #44953

    Purpose

    Yeast tagging vector to create gene fusion with yeast optimized mRuby2 with Kan selection

    Depositor

    Wendell Lim, Kurt Thorn

    Article

    Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

    Insert

    yomRuby2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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