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GFP-mouse vinculin full length (889)
(Plasmid #67935)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 67935 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Total vector size (bp) 7900
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Vinculin
  • Alt name
    Vcl
  • Alt name
    movin
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3200
  • Mutation
    L159P in vinculin
  • Entrez Gene
    Vcl (a.k.a. 9430097D22, AA571387, AI462105, AW545629)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GFP-mouse vinculin full length (889) was a gift from Alpha Yap (Addgene plasmid # 67935 ; http://n2t.net/addgene:67935 ; RRID:Addgene_67935)
  • For your References section:

    Tension-sensitive actin assembly supports contractility at the epithelial zonula adherens. Leerberg JM, Gomez GA, Verma S, Moussa EJ, Wu SK, Priya R, Hoffman BD, Grashoff C, Schwartz MA, Yap AS. Curr Biol. 2014 Aug 4;24(15):1689-99. doi: 10.1016/j.cub.2014.06.028. Epub 2014 Jul 24. 10.1016/j.cub.2014.06.028 PubMed 25065757