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pmGFP11C-­Tau
(Plasmid #71434)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 71434 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pmGFP11C
  • Total vector size (bp) 5200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Tau (0N4R)
  • Species
    H. sapiens (human)
  • Entrez Gene
    MAPT (a.k.a. DDPAC, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, tau-40)
  • Tag / Fusion Protein
    • GFP (213-228) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI/NheI (destroyed during cloning)
  • 3′ cloning site KpnI (destroyed during cloning)
  • 5′ sequencing primer unknown
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmGFP11C-­Tau was a gift from Henri Huttunen (Addgene plasmid # 71434 ; http://n2t.net/addgene:71434 ; RRID:Addgene_71434)
  • For your References section:

    Internalized Tau sensitizes cells to stress by promoting formation and stability of stress granules. Brunello CA, Yan X, Huttunen HJ. Sci Rep. 2016 Jul 27;6:30498. doi: 10.1038/srep30498. 10.1038/srep30498 PubMed 27460788