Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pmGFP10C-­Tau
(Plasmid #71433)

Loading...

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 71433 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Tau (0N4R)
  • Species
    H. sapiens (human)
  • Entrez Gene
    MAPT (a.k.a. DDPAC, FTDP-17, MAPTL, MSTD, MTBT1, MTBT2, PPND, PPP1R103, TAU, tau-40)
  • Tag / Fusion Protein
    • GFP (1-212) (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SpeI/NheI (destroyed during cloning)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer Forw (T7): 5'-­‐TAATACGACTCACTATAGGG-­‐3'
  • 3′ sequencing primer Rev: 5'-­‐TAGAAGGCACAGTCGAGG-­‐3'
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmGFP10C-­Tau was a gift from Henri Huttunen (Addgene plasmid # 71433 ; http://n2t.net/addgene:71433 ; RRID:Addgene_71433)
  • For your References section:

    Internalized Tau sensitizes cells to stress by promoting formation and stability of stress granules. Brunello CA, Yan X, Huttunen HJ. Sci Rep. 2016 Jul 27;6:30498. doi: 10.1038/srep30498. 10.1038/srep30498 PubMed 27460788