|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||72299||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 6677
Modifications to backboneClaI/SalI fragment was replaced with CB promoter + MCS
Vector typeMammalian Expression, Lentiviral
- Promoter chicken beta-actin + CMV enhancer
Growth in Bacteria
Growth Strain(s)NEB Stable
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer CGGTGGTGGTGCAAATCAAA
- 3′ sequencing primer CAACACCACGGAATTGTCAG (Common Sequencing Primers)
The depositor noted that the single nucleotide mismatch (G>A, bp 3249) within IRES found in Addgene's quality control sequence does NOT affect plasmid function.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCDH-CB-IRES-copGFP-T2A-Puro was a gift from Kazuhiro Oka (Addgene plasmid # 72299 ; http://n2t.net/addgene:72299 ; RRID:Addgene_72299)