169,691 results
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Plasmid#121746PurposepMVP L3-L2 entry plasmid, contains human growth hormone polyadenylation signal (polyA) for 3- or 4-component MultiSite Gateway Pro assemblyDepositorInsertPolyadenylation signal (polyA)
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFRT-DHX36iso1
Plasmid#159585PurposeExpress N-ter FLAG HA tagged DHX36 isoform 1 in mammalian cellsDepositorAvailable SinceMay 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
AAV-pCBh-ABE8e-nSpCas9(D10A)[N-term] (HES1468)
Plasmid#197509PurposeCbh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-nSpCas9(D10A)DepositorInsertAAV-[ITR]-pCBh-BPNLS-TadA8e-nSpCas9(D10A)[N-term]-NpuN-BPNLS-[ITR]
UseAAV and CRISPRTagsBPNLS and NpuN(intein)-BPNLSMutationABE8e mutations in TadA and nSpCas9(D10A)PromoterCbhAvailable SinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
mEos3.2-N1
Plasmid#54525PurposeLocalization: N1 Cloning Vector, Excitation: 507 / 572, Emission: 516 / 580DepositorTypeEmpty backboneTagsmEos3.2ExpressionMammalianPromoterCMVAvailable SinceJune 12, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
NanoLuc-KRAS(G12D)_CRAF(FL)-HaloTag BiBRET Vector
Plasmid#238579PurposeExpress NanoLuc-KRAS(G12D)_CRAF(FL)-HaloTag Fusion Protein in Mammalian Cells under a CMV promoterDepositorHas ServiceDNATagsHaloTag (R) and NanoLuc (R)ExpressionMammalianMutationG12DPromoterCMVAvailable SinceSept. 14, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLenti-EF1a-hMLH1dn-eGFP
Plasmid#191104PurposeLentiviral expression plasmid of hMLH1dn with P2A-eGFP reporterDepositorInserthMLH1dn
UseLentiviralExpressionMammalianPromoterEF-1aAvailable SinceMay 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFH3.3_Cre-MS2x12
Plasmid#205559PurposeExpress Cre-MS2x12 (export tag consisting of 12 tandem repeats of MS2 stem-loop aptamer)DepositorInsertCre
ExpressionMammalianMutationWTPromoterCMVAvailable SinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hsyn-ASAP5-Kv2.1-WPRE
Plasmid#225707PurposePan-neuronal soma-targeted expression of the genetically encoded voltage indicator ASAP5DepositorHas ServiceAAV1InsertASAP5-Kv2.1
UseAAVPromoterhuman synapsinAvailable SinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1137
Plasmid#84827PurposeMinimos transposon with Peft-3:GFP:tbb-2 3'UTR and cbr-unc-119 selection. GFP was optimized for C. elegans, contains 2x NLS (SV40/egl-13), and synthetic intronsDepositorInsertC. elegans codon optimized GFP
ExpressionBacterial and WormPromoterPeft-3Available SinceDec. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro
Plasmid#183409PurposepiggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cellsDepositorInsertdCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1
UseCRISPR and Synthetic Biology; PiggybacExpressionMammalianPromoterTRE3GAvailable SinceMay 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJW1511
Plasmid#62365PurposepEF1a-puro-T2A-ATHmmuRPL10ADepositorInsertPuro-T2A-AVITEVHAmmuRPL10A
UseLentiviralExpressionMammalianPromoterEF1aAvailable SinceMarch 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
TFORF2788
Plasmid#144238PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_ATPIF1_WT
Plasmid#81774PurposeGateway Donor vector containing ATPIF1 ,part of the Target Accelerator Plasmid Collection.DepositorAvailable SinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
ePE6d
Plasmid#229029PurposeExpresses ePE6d — engineered SpCas9 nickase (nCas9), RNA chaperone nucleocapsid (NC) protein, with T128N/N200C/V223Y variant of MMLVΔRNaseH — for gene editing in riceDepositorInsertCas9
UseCRISPRAvailable SinceFeb. 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
KP201: pMVP (L5-L4) eGFP-P2A
Plasmid#121708PurposepMVP L5-L4 entry plasmid, contains eGFP-P2A for 4-component MultiSite Gateway Pro assembly. Allows expression of N-term eGFP linked by P2A to gene of interest.DepositorInserteGFP-P2A (open)
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-QUAS:GCaMP7a
Plasmid#184817PurposeUsed to generate the Tg(QUAS:GCaMP7a)c594 transgenic line. GCaMP7a from Muto et al., 2013 (https://doi.org/10.1016/j.cub.2012.12.040).DepositorInsertQUAS:GCaMP7a-pA
UseZebrafish tol2 transgenesisAvailable SinceMay 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMGE-T7
Plasmid#153021PurposeSingle-plasmid multi-gene expression system. Each genes under the control of a T7 and the lacO promoter sequences.DepositorTypeEmpty backboneExpressionBacterialPromoterT7Available SinceJune 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTwist-SARS-CoV-2 Spike-deltaC18 KP.2
Plasmid#223273PurposeEncodes SARS-CoV-2 spike KP.2 for pseudovirus productionDepositorInsertSARS-CoV-2 Spike KP.2 (S Severe acute respiratory syndrome coronavirus 2)
ExpressionMammalianMutationSee Depositor Comments.PromoterCAGAvailable SinceDec. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLX304 GFP
Plasmid#193687PurposeConstitutive lentiviral expression of GFPDepositorInsertGFP
UseLentiviralAvailable SinceDec. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCAG-hSt3Cas9-NLS(SV40)-3xFLAG (KAC426)
Plasmid#133788PurposeCAG promoter expression plasmid for human codon optimized St3Cas9 nuclease with C-terminal NLS (SV40)DepositorInserthuman codon optimized St3Cas9
TagsNLS(SV40)-3xFLAGExpressionMammalianMutationn/aPromoterCAGAvailable SinceMarch 30, 2020AvailabilityAcademic Institutions and Nonprofits only