159,557 results

1. pSQT1601

   Plasmid

   #53369

   Purpose

   human codon optimized Csy4 and FokI-dCas9 expression plasmid

   Depositor

   Keith Joung

   Article

   Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   hCsy4-T2A-NLS-hFokI-dCas9-NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   June 19, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. TRE-mCherry-p2A-eNpHR3.0-TS

   Plasmid

   #158703

   Purpose

   Reporter construct

   Depositor

   Alice Ting

   Article

   Sanchez et al Proc Natl Acad Sci U S A. 2020 Dec 15. p

   Insert

   mCherry-p2A-eNpHR3.0-TS
   

   Use

   AAV

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   TRE

   Available since

   Jan. 6, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. mouse E-cadherin GFP (423)

   Plasmid

   #67937

   Purpose

   Mammalian expression of E-cadherin-GFP

   Depositor

   Alpha Yap

   Article

   Truffi et al J Cell Sci. 2014 Jun 1;127(Pt 11):2420-3

   Insert

   E-cadherin (Cdh1 Mouse)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLL7.0: Venus-iLID-Mito (From ActA)

   Plasmid

   #60413

   Purpose

   Mammalian Expression of Venus-iLID-Mito

   Depositor

   Brian Kuhlman

   Article

   Guntas et al Proc Natl Acad Sci U S A. 2015 Jan 6;112

   Insert

   Venus-iLID-Mito
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Venus

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 27, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pICE-HA-MRE11-H63D

   Plasmid

   #82036

   Purpose

   Plasmid for constitutive or doxycycline-inducible expression of H63D exonuclease-dead mutant of human MRE11. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.

   Depositor

   Sébastien Britton, Patrick Calsou

   Article

   Chanut et al Nat Commun. 2016 Sep 19;7:12889. doi: 10

   Insert

   MRE11 (MRE11 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Exonuclease-dead mutant: H63D MRE11

   Promoter

   CMV-tet

   Available since

   Sept. 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLKO-TRE-AFOS

   Plasmid

   #197079

   Purpose

   A doxycycline inducible dominant negative form of transcription complex AP1. AFOS heterodimerizes and inhibits c-Jun. Plasmid contains PGK driven H2B-RFP as a fluorescent reporter of transduction.

   Depositor

   Elaine Fuchs

   Article

   Larsen et al Cell Stem Cell. 2021 Oct 7;28(10):1758-1

   Insert

   AFOS (Fos Mouse)
   

   Use

   Lentiviral

   Tags

   Flag and T7

   Expression

   Mutation

   Promoter

   TRE

   Available since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-nEFCas9

   Plasmid

   #87115

   Purpose

   SpCas9 expression AAV backbone plasmid under control of nEF promoter

   Depositor

   Juan Belmonte

   Article

   Suzuki et al Nature. 2016 Dec 1;540(7631):144-149. do

   Insert

   nEF-Cas9
   

   Use

   AAV and CRISPR

   Tags

   HA-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 30, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCLASP_mScarlet_CLASP_RGS2membrane

   Plasmid

   #133086

   Purpose

   Plasmid contains mScarlet with CLASP. This consists of the plasma membrane anchor (pm-LOVTRAP) and the cargo (Zdk-mScarlet-yeLANS). CLASP modulates nuclear localization of mScarlet in response to bl

   Depositor

   Hana El-Samad

   Article

   Chen et al Cell Syst. 2020 Sep 4. pii: S2405-4712(2

   Insert

   mScarlet-CLASP
   

   Use

   Tags

   Expression

   Bacterial and Yeast

   Mutation

   Promoter

   Available since

   Sept. 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLminP_Luc2P_RE59

   Plasmid

   #90404

   Purpose

   STAT3 - Sis-inducible element (SIE) gene reporter

   Depositor

   Ramnik Xavier

   Article

   O'Connell et al Cell Syst. 2016 May 25;2(5):323-34. doi:

   Insert

   Firefly luciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   IRF1

   Available since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-V5-DGCR8

    Plasmid

    #51383

    Depositor

    Narry Kim

    Article

    Yeom et al Nucleic Acids Res. 2006;34(16):4622-9. E

    Insert

    DGCR8 (DGCR8 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 31, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. Tom20-V5-FKBP-AP_pLX304

    Plasmid

    #120914

    Purpose

    Lentiviral vector expressing Tom20-V5-FKBP-AP on mammalian mitochondrial outer membrane

    Depositor

    Alice Ting

    Article

    Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

    Insert

    Tom20-V5-FKBP-AP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. MAC-tag-N

    Plasmid

    #108078

    Purpose

    Gateway compatible destination vectors containing StrepIII/HA/BioID

    Depositor

    Markku Varjosalo

    Article

    Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

    Insert

    CCDB (ccdB(letD) )
    

    Use

    Tags

    StrepIII-HA-BirA*

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA5_FRT_GFP

    Plasmid

    #127108

    Depositor

    Thomas Tuschl

    Article

    Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

    Insert

    GFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pENTR4-V5 (w71-3)

    Plasmid

    #17425

    Purpose

    Gateway entry vector for N-term V5 tag, frame A

    Depositor

    Paul Kaufman, Eric Campeau

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Type

    Empty backbone

    Use

    Entry vector

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pR_LG lenti-(empty)-del-CTS

    Plasmid

    #112895

    Purpose

    Non-integrating lentiviral co-packaging plasmid designed to reduce multiple integrations and recombination during transduction of nucleic acid libraries

    Depositor

    Paul Blainey

    Article

    Feldman et al bioRxiv 262121

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    none

    Available since

    Sept. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pZS160 CRISPEY HDV-HDV NotI

    Plasmid

    #114453

    Purpose

    Yeast Centromere Plasmid for galactose inducible expression of retron-guide chimeric RNA with flanking HDV-HDV ribozyme

    Depositor

    Hunter Fraser

    Article

    Sharon et al Cell. 2018 Sep 18. pii: S0092-8674(18)31

    Type

    Empty backbone

    Use

    CRISPR; Cen/ars

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    ScGAL7p

    Available since

    Oct. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRS423-GPD-wrmScarlet1-10-CYC1 terminator

    Plasmid

    #158584

    Purpose

    Expresses wrmScarlet1-10 in S. cerevisiae

    Depositor

    Cynthia Kenyon

    Article

    Goudeau et al Genetics. 2021 Apr 15;217(4). pii: 61264

    Insert

    wrmScarlet1-10
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GPD

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. EF1a-mEmerald-CAPRIN1

    Plasmid

    #164218

    Purpose

    Expresses mEmerald tagged CAPRIN1 under EF-1a promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    CAPRIN1 (CAPRIN1 Human)
    

    Use

    Lentiviral

    Tags

    mEmerald

    Expression

    Mutation

    Promoter

    EF-1a

    Available since

    June 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. SIRT6 Flag

    Plasmid

    #13817

    Purpose

    Mammalian expression of human SIRT6 with flag tag

    Depositor

    Eric Verdin

    Article

    North et al Mol Cell. 2003 Feb . 11(2):437-44.

    Insert

    SIRT6 (SIRT6 Human)
    

    Use

    Tags

    flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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20. HCN4/EGFP-C1

    Plasmid

    #197389

    Purpose

    Plasmid expressing an antigen targeting the HCN4 ion channel subunit

    Depositor

    Dane Chetkovich

    Insert

    HCN4 (HCN4 Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge