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SpySwitch
(Plasmid #184225)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 184225 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDEST14
  • Backbone manufacturer
    Thermo Fisher Scientific
  • Backbone size w/o insert (bp) 3641
  • Total vector size (bp) 4055
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpySwitch
  • Species
    Synthetic
  • Insert Size (bp)
    414
  • Mutation
    E77A mutation prevents isopeptide bond formation, S49C mutation enables attachment to solid support. K28H, S30H, R32H, S59T, Y84H, E85H, V94I and A111P allow for capture and gentle pH or temperature release of SpyTag fusion proteins.
  • Promoter T7
  • Tag / Fusion Protein
    • His6 (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SpySwitch was a gift from Mark Howarth (Addgene plasmid # 184225 ; http://n2t.net/addgene:184225 ; RRID:Addgene_184225)
  • For your References section:

    SpySwitch enables pH- or heat-responsive capture and release for plug-and-display nanoassembly. Vester SK, Rahikainen R, Khairil Anuar INA, Hills RA, Tan TK, Howarth M. Nat Commun. 2022 Jun 28;13(1):3714. doi: 10.1038/s41467-022-31193-8. 10.1038/s41467-022-31193-8 PubMed 35764623