SpySwitch enables pH- or heat-responsive capture and release for plug-and-display nanoassembly.
Vester SK, Rahikainen R, Khairil Anuar INA, Hills RA, Tan TK, Howarth M
Nat Commun. 2022 Jun 28;13(1):3714. doi: 10.1038/s41467-022-31193-8. PubMed Article
Vester SK, Rahikainen R, Khairil Anuar INA, Hills RA, Tan TK, Howarth M
Nat Commun. 2022 Jun 28;13(1):3714. doi: 10.1038/s41467-022-31193-8. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 184225 | SpySwitch | Expresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release. |
| 184226 | SpyTag-sfGFP | Expresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher. |
| 184227 | AviTag-SpyTag003-MBP | Expresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003. |