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SpySwitch enables pH- or heat-responsive capture and release for plug-and-display nanoassembly.
Vester SK, Rahikainen R, Khairil Anuar INA, Hills RA, Tan TK, Howarth M
Nat Commun. 2022 Jun 28;13(1):3714. doi: 10.1038/s41467-022-31193-8.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
184225SpySwitchExpresses SpySwitch in the bacterial cytoplasm. SpySwitch binds SpyTag-, SpyTag002- or SpyTag003-fusions non-covalently for affinity purification, allowing gentle pH or temperature release.
184226SpyTag-sfGFPExpresses SpyTag-superfolder GFP in the bacterial cytoplasm. SpyTag enables covalent reaction with SpyCatcher.
184227AviTag-SpyTag003-MBPExpresses AviTag and SpyTag003 linked to maltose binding protein in the bacterial cytoplasm. AviTag enables site-specific biotinylation by BirA. SpyTag003 enables reaction with SpyCatcher003.

Antibodies from Article