Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pSecTag-ATX
(Plasmid #17839)

Loading...

Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 17839 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSecTag
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 5200
  • Vector type
    Mammalian Expression
  • Selectable markers
    Zeocin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Autotaxin
  • Alt name
    ATX
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3000
  • Entrez Gene
    ENPP2 (a.k.a. ATX, ATX-X, AUTOTAXIN, LysoPLD, NPP2, PD-IALPHA, PDNP2)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

From the depositor:

In 1998, we purchased from Invitrogen pSecTag2A and cut it with BglII and BamH1. The BglII/BamH1 fragment was replaced with a modified fragment containing 6 Histidines at the N-Terminus that was done by generating the modification by PCR, and cloning the PCR product back into BglII/BamH1.
Sometime later, the above pSecTag2A-6HIS was digested with BamH1and Not1. The ATX (amino acids 52-863) insert was genereated by PCR, digested with BamH1/EcoR1 and by excising ATX from the original colony II cDNA library with EcoR1/Not1, which yields two pieces in two stages into the vector.
Their is a stop codon before the C-Terminus Myc tag so it will not be expressed.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSecTag-ATX was a gift from Steven Rosen (Addgene plasmid # 17839 ; http://n2t.net/addgene:17839 ; RRID:Addgene_17839)
  • For your References section:

    Autotaxin, an ectoenzyme that produces lysophosphatidic acid, promotes the entry of lymphocytes into secondary lymphoid organs. Kanda H, Newton R, Klein R, Morita Y, Gunn MD, Rosen SD. Nat Immunol. 2008 Apr . 9(4):415-23. 10.1038/ni1573 PubMed 18327261