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(Plasmid #24304)


Item Catalog # Description Quantity Price (USD)
Plasmid 24304 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Naldini/Trono (Addgene #12252)
  • Backbone size w/o insert (bp) 6132
  • Vector type
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    7xTcf-eGFP // SV40-mCherry
  • Alt name
  • Alt name
  • Alt name
    enhanced GFP
  • Species
    Aequorea victora (modified) / synthetic
  • Insert Size (bp)
  • Mutation
    EGFP is GFP with S65T and F64L mutations

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PstI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer GGCCCGAAGGAATAGAAG
  • 3′ sequencing primer AACGGGCCACAACTCCTC
  • (Common Sequencing Primers)

Resource Information

  • Addgene Notes
  • A portion of this plasmid was derived from a plasmid made by
    The 7xTcf promoter is derived from pSuperTOPflash, published in Veeman MT et al., Curr Biol 13: 680–685 (2003)
  • Articles Citing this Plasmid

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

TCF repeat sequence is AGATCAAAGG

This plasmid requires a 2nd generation lentiviral packaging system.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    7TGC was a gift from Roel Nusse (Addgene plasmid # 24304 ; ; RRID:Addgene_24304)
  • For your References section:

    Lentiviral vectors to probe and manipulate the wnt signaling pathway. Fuerer C, Nusse R. PLoS One. 2010 . 5(2):e9370. 10.1371/journal.pone.0009370 PubMed 20186325
Commonly requested with: