Intron targeting-mediated and endogenous gene integrity-maintaining knockin in zebrafish using the CRISPR/Cas9 system.
Li J, Zhang BB, Ren YG, Gu SY, Xiang YH, Huang C, Du JL
Cell Res. 2015 Apr 7. doi: 10.1038/cr.2015.43.
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PubMed
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 65562 | th-P2A-EGFP donor | Intron targeting-mediated EGFP knockin at the zebrafish th locus. |
| 65563 | th-P2A-Gal4 donor | Intron targeting-mediated Gal4 knockin at the zebrafish th locus. |
| 65564 | gfap-EGFP donor | Intron targeting-mediated EGFP knockin at the zebrafish gfap locus |
| 65565 | pT7-th-sgRNA | in vitro trancription of sgRNA targeting the zebrafish th locus |
| 65566 | pT7-gfap-sgRNA | in vitro trancription of sgRNA targeting the zebrafish gfap locus |