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Addgene

th-P2A-Gal4 donor
(Plasmid #65563)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 65563 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    PMD-19-T
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 2692
  • Total vector size (bp) 5429
  • Vector type
    zebrafish injection

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    th-P2A-Gal4
  • Alt name
    th (left arm)-P2A-Gal4-th (right arm)
  • Species
    D. rerio (zebrafish)
  • Entrez Gene
    th
  • Promoter none

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    th-P2A-Gal4 donor was a gift from Jiu-lin Du (Addgene plasmid # 65563 ; http://n2t.net/addgene:65563 ; RRID:Addgene_65563)
  • For your References section:

    Intron targeting-mediated and endogenous gene integrity-maintaining knockin in zebrafish using the CRISPR/Cas9 system. Li J, Zhang BB, Ren YG, Gu SY, Xiang YH, Huang C, Du JL. Cell Res. 2015 Apr 7. doi: 10.1038/cr.2015.43. 10.1038/cr.2015.43 PubMed 25849248