PurposeIntron targeting-mediated EGFP knockin at the zebrafish gfap locus
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||65564||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 2692
- Total vector size (bp) 5429
Vector typezebrafish injection
Growth in Bacteria
Alt namegfap (left arm)-EGFP-gfap (right arm)
SpeciesD. rerio (zebrafish)
Entrez Genegfap (a.k.a. cb345, etID36982.3, gfapl, wu:fb34h11, wu:fk42c12, xx:af506734, zgc:110485)
- Promoter none
- Cloning method TOPO Cloning
- 5′ sequencing primer M13pUC-fwd
- 3′ sequencing primer M13 Reverse (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
P2A sequence was replaced with a flexible serine-serine linker sequence (TCTTCT)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:gfap-EGFP donor was a gift from Jiu-lin Du (Addgene plasmid # 65564 ; http://n2t.net/addgene:65564 ; RRID:Addgene_65564)
For your References section:Intron targeting-mediated and endogenous gene integrity-maintaining knockin in zebrafish using the CRISPR/Cas9 system. Li J, Zhang BB, Ren YG, Gu SY, Xiang YH, Huang C, Du JL. Cell Res. 2015 Apr 7. doi: 10.1038/cr.2015.43. 10.1038/cr.2015.43 PubMed 25849248