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pLenti CMVie-IRES-BlastR
(Plasmid #119863)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 119863 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCIG3
  • Backbone size (bp) 7298
  • Modifications to backbone
    Replaced GFP with Blasticidin Resistance
  • Vector type
    Mammalian Expression, Lentiviral
  • Promoter CMV immediate early
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Modification of empty lentiviral vector pCIG3 (Addgene 78264), Goodrum Lab
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that though there are some discrepancies between the backbone sequence from the depositing lab and Addgene's quality control sequence, this plasmid should function as described.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti CMVie-IRES-BlastR was a gift from Ghassan Mouneimne (Addgene plasmid # 119863 ; http://n2t.net/addgene:119863 ; RRID:Addgene_119863)
  • For your References section:

    Mechanosensing during directed cell migration requires dynamic actin polymerization at focal adhesions. Puleo JI, Parker SS, Roman MR, Watson AW, Eliato KR, Peng L, Saboda K, Roe DJ, Ros R, Gertler FB, Mouneimne G. J Cell Biol. 2019 Oct 8. pii: jcb.201902101. doi: 10.1083/jcb.201902101. 10.1083/jcb.201902101 PubMed 31594807