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pSKB3SaGGR G91H (JBEI-3677)

Plasmid

#110148

Purpose

SaGGR site-directed mutation of G91H

Depositor

Jay Keasling

Article

Kung et al Structure. 2014 Jul 8;22(7):1028-36. doi

Insert

GGR

Expression

Bacterial

Mutation

SaGGR site-directed mutation of G91H

Promoter

T7

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pSKB3SaGGR L377H (JBEI-3683)

Plasmid

#110150

Purpose

SaGGR site-directed mutation of L377H

Depositor

Jay Keasling

Article

Kung et al Structure. 2014 Jul 8;22(7):1028-36. doi

Insert

GGR

Expression

Bacterial

Mutation

SaGGR site-directed mutation of L377H

Promoter

T7

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCRRNA-G11-R0

Plasmid

#101805

Purpose

Repress mCherry and GFP independently, with different strengths

Depositor

David Bikard, Sven van Teeffelen

Article

Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

Insert

crRNAs targeting GFP and mCherry

Use

CRISPR and Synthetic Biology

Promoter

Spy 1049

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pEHIShqprtR161Q

Plasmid

#92413

Purpose

hQPRTase harboring R161Q mutation

Depositor

Huanting Liu, James Naismith

Article

Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

Insert

hQPRTase (QPRT Human)

Tags

TEV protease cleavable 6xHis

Expression

Bacterial

Mutation

R161Q

Promoter

T7

Available Since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pCRRNA-G14-R0

Plasmid

#101806

Purpose

Repress mCherry and GFP independently, with different strengths

Depositor

David Bikard, Sven van Teeffelen

Article

Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

Insert

crRNAs targeting GFP and mCherry

Use

CRISPR and Synthetic Biology

Promoter

Spy 1049

Available Since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pCRRNA-G10-R0

Plasmid

#101804

Purpose

Repress mCherry and GFP independently, with different strengths

Depositor

David Bikard, Sven van Teeffelen

Article

Vigouroux et al Mol Syst Biol. 2018 Mar 8;14(3):e7899.

Insert

crRNAs targeting GFP and mCherry

Use

CRISPR and Synthetic Biology

Promoter

Spy 1049

Available Since

Oct. 24, 2017

Availability

Academic Institutions and Nonprofits only

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YIp128-P30-His-pol30(K127/164R)

Plasmid

#99545

Purpose

Yeast integrative vector for expression of His-tagged POL30 (PCNA) mutant K127/164R; Leu2 marker

Depositor

Helle Ulrich

Article

Davies et al Methods Mol Biol. 2012;920:543-67. doi:

Insert

POL30 (POL30 Budding Yeast)

Tags

6xHis

Expression

Yeast

Mutation

K127R, K164R

Promoter

POL30

Available Since

Sept. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pEHIAhqprtR161G

Plasmid

#92412

Purpose

hQPRTase harbouring R161G mutation

Depositor

Huanting Liu, James Naismith

Article

Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

Insert

hQPRTase (QPRT Human)

Tags

TEV protease cleavable 6xHis

Expression

Bacterial

Mutation

R161G

Promoter

T7

Available Since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pEHIShqprtR102Q

Plasmid

#92407

Purpose

hQPRTase harboring R102Q mutation

Depositor

Huanting Liu, James Naismith

Article

Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

Insert

hQPRTase (QPRT Human)

Tags

TEV protease cleavable 6xHis

Expression

Bacterial

Mutation

R102Q

Promoter

T7

Available Since

Aug. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pEHIShqprtK171S

Plasmid

#92404

Purpose

hQPRTase harbouring K171S mutation

Depositor

Huanting Liu, James Naismith

Article

Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

Insert

hQPRTase (QPRT Human)

Tags

TEV protease cleavable 6xHis

Expression

Bacterial

Mutation

K171S

Promoter

T7

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pEHIShqprtR138Q

Plasmid

#92411

Purpose

hQPRTase harbouring R138Q mutation

Depositor

Huanting Liu, James Naismith

Article

Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

Insert

hQPRTase (QPRT Human)

Tags

TEV protease cleavable 6xHis

Expression

Bacterial

Mutation

R138Q

Promoter

T7

Available Since

Aug. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pEHIShqprtR102A

Plasmid

#92405

Purpose

hQPRTASE harbouring R102A mutation

Depositor

Huanting Liu, James Naismith

Article

Liu et al J Mol Biol. 2007 Oct 26;373(3):755-63. E

Insert

hQPRTase (QPRT Human)

Tags

TEV protease cleavable 6xHis

Expression

Bacterial

Mutation

R102A

Promoter

T7

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;cldn5bCE1-basEgfp

Plasmid

#90136

Purpose

contains cldn5b enhancer sequence and a basal promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

cldn5bE1basEgfp (cldn5b Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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pMEsnx5P1gfp

Plasmid

#90131

Purpose

Gateway Mid-entry clone containing promoter sequence specific for snx5 driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

snx5P1Egfp (snx5 Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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p5Esnx5E1

Plasmid

#90115

Purpose

5' Gateway entry clone containing endothelial specific enhancer sequence of snx5

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

snx5E1 (snx5 Zebrafish)

Expression

Bacterial

Available Since

June 29, 2017

Availability

Academic Institutions and Nonprofits only

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pACYC_HA-ZmSAE1_ZmSAE2(TRUNC)-FLAG

Plasmid

#91945

Purpose

Bacterial expression of maize SAE1 and truncated SAE2

Depositor

Richard Vierstra

Article

Augustine et al Plant Physiol. 2016 Jul;171(3):2191-210.

Inserts

HA-ZmSAE1
ZmSAE2-FLAG

Tags

FLAG and HA

Expression

Bacterial

Mutation

Truncation of ZmSAE2 to mimic a splice variant

Available Since

June 28, 2017

Availability

Academic Institutions and Nonprofits only

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LLhG-pHG165a

Plasmid

#90039

Purpose

Constitutive expression of a chimeric LacI:GalR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Meinhardt et al Proteins. 2008 Dec;73(4):941-57. doi: 10

Insert

LLhG

Expression

Bacterial

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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LGhG-pHG165a

Plasmid

#90048

Purpose

Constitutive expression of a chimeric LacI:GalR transcription repressor

Depositor

Liskin Swint-Kruse

Article

Tungtur et al J Mol Biol. 2010 Jan 29;395(4):785-802.

Insert

LGhG

Expression

Bacterial

Promoter

Iq

Available Since

June 23, 2017

Availability

Academic Institutions and Nonprofits only

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pMEtmem88aP1gfp

Plasmid

#90132

Purpose

Gateway Mid-entry clone containing promoter sequence specific for tmem88a driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

tmem88aP1Egfp (tmem88a Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

June 16, 2017

Availability

Academic Institutions and Nonprofits only

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pT2-cryR;yrkP1Egfp

Plasmid

#90168

Purpose

contains yrk specific gene promoter driving expression of eGFP

Depositor

Nathan Lawson

Article

Quillien et al Cell Rep. 2017 Jul 18;20(3):709-720. doi

Insert

yrkP1Egfp (yrk Zebrafish)

Tags

EGFP

Expression

Bacterial

Available Since

May 24, 2017

Availability

Academic Institutions and Nonprofits only

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