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pMYT062

Plasmid

#180715

Purpose

Spacer cassette with ConL3 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

3/E Spacer Cassette

Use

Synthetic Biology

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT061

Plasmid

#180714

Purpose

Spacer cassette with ConL2 and ConR3 connectors to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

2/3 Spacer Cassette

Use

Synthetic Biology

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT060

Plasmid

#180713

Purpose

Spacer cassette with ConL2 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

2/E Spacer Cassette

Use

Synthetic Biology

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT059

Plasmid

#180712

Purpose

Spacer cassette with ConL1 and ConR2 connectors to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

1/2 Spacer Cassette

Use

Synthetic Biology

Available Since

Aug. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pILGFP1D5

Plasmid

#165063

Purpose

Characterisation of yeast promoter using yEGFP as reporter gene and PGK1 terminator

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Type

Empty backbone

Expression

Yeast

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT058

Plasmid

#180711

Purpose

Spacer cassette with ConL1 and ConRE connectors to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

1/E Spacer Cassette

Use

Synthetic Biology

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pMYT057

Plasmid

#180710

Purpose

Spacer cassette with ConLS and ConR1 connectors to be used in the Dueber YTK and Ellis MYT systems

Depositor

Tom Ellis

Article

Shaw et al ACS Synth Biol. 2023 Nov 6. doi: 10.1021

Insert

S/1 Spacer Cassette

Use

Synthetic Biology

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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InterTag-3xL-Δtag

Plasmid

#186914

Purpose

Sleeping Beauty transposon system with constitutive bi-directional promoter, expressing sfCherry11 fused to PDGFR through a (GGGGS)3 linker and the other side BFP and puromycin resistance gene

Depositor

Taekjip Ha, Allen Liu

Article

Moghimianavval et al Small. 2022 May 26:e2202104. doi: 10.100

Insert

sfCherry11-(GGGGS)3-PDGFR

Expression

Mammalian

Available Since

Aug. 16, 2022

Availability

Academic Institutions and Nonprofits only

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HEpl016

Plasmid

#179991

Purpose

pCBh_3xFLAG_NLS_SiCas12i_NLS_pA_pCMV_mCherry_pU6_crRNA_BbsI: vector for encoding a human codon-optimized SiCas12i driven by CBh promoter and tagmCherry driven by CMV promoter

Depositor

Yingsi Zhou

Article

Zhang et al Protein Cell. 2023 Jun 28;14(7):538-543.

Insert

SiCas12i

Tags

3xFlag

Expression

Mammalian

Available Since

April 4, 2022

Availability

Academic Institutions and Nonprofits only

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pIR3DH8

Plasmid

#165068

Purpose

Knocking out GAL80 using KlURA3 marker

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

gal80Arm1-P-AgTEF1-KlURA3-T-AgTEF1-gal80Arm2

Expression

Yeast

Mutation

WT

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pNK083d

Plasmid

#156367

Purpose

Expresses heterlogous ribosomal proteins

Depositor

Ahmed Badran

Article

Kolber et al Nat Commun. 2021 Jan 26;12(1):599. doi:

Insert

S20, S16 ribosomal genes

Use

Synthetic Biology

Available Since

Nov. 19, 2020

Availability

Academic Institutions and Nonprofits only

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pGD318

Plasmid

#129735

Purpose

Contains a myristoylation sequence in the pGGC000 backbone (GreenGate)

Depositor

Rüdiger Simon

Article

Denay et al Plant Direct. 2019 Dec 6;3(12):e00189. d

Insert

Myristoylation sequence (MGRRKRKPK-)

Use

Greengate entry plasmid

Available Since

Oct. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.284

Plasmid

#119650

Purpose

Level 0 Part. Insertion site

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

7002 NS1 DOWN Flank

Use

Synthetic Biology

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.282

Plasmid

#119648

Purpose

Level 0 Part. Insertion site

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

7002 NS2 DOWN Flank

Use

Synthetic Biology

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.172

Plasmid

#119640

Purpose

Level 0 Part. Insertion site

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

6803 NS4 Down Flank

Use

Synthetic Biology

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.170

Plasmid

#119638

Purpose

Level 0 Part. Insertion site

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

6803 NS3 Down Flank

Use

Synthetic Biology

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.166

Plasmid

#119633

Purpose

Level 0 Part. Insertion site

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

6803 NS1 Down Flank

Use

Synthetic Biology

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.025

Plasmid

#119631

Purpose

Level 0 Part. Insertion site

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

6803 cpcBAC1&C2 Down Flank

Use

Synthetic Biology

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.161

Plasmid

#119620

Purpose

Level 0 Part. CDS

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

eYFP C-term tag

Use

Synthetic Biology

Mutation

bp 242 T to A

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pC0.009

Plasmid

#119619

Purpose

Level 0 Part. CDS

Depositor

Alistair McCormick

Article

Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

Insert

eYFP+deg-tag

Use

Synthetic Biology

Mutation

bp 242 T to A

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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