We narrowed to 10,139 results for: yeast

1. pFA6-CFAPoptim-NAT

   Plasmid

   #221129

   Purpose

   Plasmid for generating integration cassette to C-terminally tag gene of interest with FAPoptim::NATMX

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Insert

   FAPoptim
   

   Expression

   Yeast

   Available Since

   April 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFA6-CFAPoptim-KAN

   Plasmid

   #221128

   Purpose

   Plasmid for generating integration cassette to C-terminally tag gene of interest with FAPoptim::KANMX

   Depositor

   Allyson O’Donnell

   Article

   Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

   Insert

   FAPoptim
   

   Expression

   Yeast

   Available Since

   April 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCas3-APOBEC1

   Plasmid

   #229536

   Purpose

   pMV_hyg encoding Cas3(wt)-rAPOBEC1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

   Depositor

   Kevin Verstrepen

   Article

   Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

   Inserts

   rAPOBEC1
   Cas3
   Uracil glycosylase inhibitor
   

   Tags

   XTEN

   Expression

   Bacterial and Yeast

   Mutation

   codon optimized for S.cerevisiae and codon optimi…

   Available Since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCas3-CDA1

   Plasmid

   #229534

   Purpose

   pMV_hyg encoding Cas3(wt)-CDA1 fusion construct and crRNA expression cassette. Respective crRNA spacer can be introduced via BsmBI restriction cloning

   Depositor

   Kevin Verstrepen

   Article

   Zimmermann et al Nat Commun. 2023 Jun 9;14(1):3389. doi:

   Inserts

   Cas3
   Cytidine deaminase
   Uracil glycosylase inhibitor
   

   Tags

   XTEN

   Expression

   Bacterial and Yeast

   Mutation

   codon optimized for S.cerevisiae and codon optimi…

   Available Since

   Jan. 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pPICZ A-SLC25A46-His (SB250)

   Plasmid

   #227599

   Purpose

   Inducible expression of His-tagged SLC25A46 in Pichia pastoris

   Depositor

   Luke Chao

   Article

   Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

   Insert

   SLC25A46 (SLC25A46 Human)
   

   Tags

   10xHis

   Expression

   Yeast

   Promoter

   AOX1

   Available Since

   Nov. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJRoC30_Th3.1

   Plasmid

   #188065

   Purpose

   FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.1)

   Depositor

   Sarel Fleishman

   Article

   Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

   Insert

   FuncLib-designed high-redox potential laccase from Trametes hirsuta
   

   Tags

   S. cerevisiae evolved α-factor prepro-leader sign…

   Expression

   Yeast

   Mutation

   20 PROSS + 4 FuncLib mutations, described in publ…

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJRoC30_Th3.7

   Plasmid

   #188066

   Purpose

   FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.7)

   Depositor

   Sarel Fleishman

   Article

   Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

   Insert

   FuncLib-designed high-redox potential laccase from Trametes hirsuta
   

   Tags

   S. cerevisiae evolved α-factor prepro-leader sign…

   Expression

   Yeast

   Mutation

   20 PROSS + 3 FuncLib mutations, described in publ…

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJRoC30_Th3.10

   Plasmid

   #188067

   Purpose

   FuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.10)

   Depositor

   Sarel Fleishman

   Article

   Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

   Insert

   FuncLib-designed high-redox potential laccase from Trametes hirsuta
   

   Tags

   S. cerevisiae evolved α-factor prepro-leader sign…

   Expression

   Yeast

   Mutation

   20 PROSS + 3 FuncLib mutations, described in publ…

   Available Since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPicZ:mTREK-1_cryst_I110D

   Plasmid

   #209640

   Purpose

   P. pastoris expression vector. It will generate the mouse TREK-1 channel (21-322, I110D) fused to a C-terminal GFP

   Depositor

   Dan Minor

   Article

   Pope et al Cell Chem Biol. 2020 May 21;27(5):511-52

   Insert

   Potassium channel subfamily K member 2 (KCNK2, TREK-1) I110D mutant
   

   Tags

   SNS- 3C_cleavage_site-TAAA-GFP

   Expression

   Yeast

   Mutation

   aa 21-322, K84R, Q85E, T86K, I88L, A89R, Q90A, A9…

   Available Since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSCL475

    Plasmid

    #219503

    Purpose

    Express Ade2, Can1 and Faa1 retron msd donor-gRNAs from a single Gal7 promoter, separated by a Csy4 recognition sequence, with an msr expressed in trans

    Depositor

    Seth Shipman

    Article

    Gonzalez-Delgado et al Nat Chem Biol. 2024 Nov;20(11):1482-1492

    Insert

    Ade2, Can1 and Faa1 retron msd donor-gRNAs separated by a Csy4 recognition sequence, with an msr expressed in trans
    

    Expression

    Yeast

    Promoter

    GAL7

    Available Since

    May 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pITESceIcmd

    Plasmid

    #218288

    Purpose

    Introduction of a cyanamide-inducible I-SceI expression cassette, use in combination with pITEdv1

    Depositor

    Claudia Vickers

    Article

    McDonnell et al bioRxiv 2024.03.26.586389

    Insert

    HO(-253, -1)-tSynth3

    Expression

    Yeast

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPAP_scFv

    Plasmid

    #183733

    Purpose

    Expresses anti-lysozyme scFv antibody

    Depositor

    Jun Ishii

    Article

    Ito et al Commun Biol. 2022 Jun 8;5(1):561. doi: 1

    Insert

    Anti-lysozyme scFv
    

    Tags

    His6-tag

    Expression

    Yeast

    Promoter

    AOX1 promoter

    Available Since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHyg-CUbo(K48R/K63R)-yeGFP-His6

    Plasmid

    #212785

    Purpose

    C-terminal PCR-based tagging with CUbo(K48R/K63R)-yeGFP-His6 in budding yeast with HygR selection

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Type

    Empty backbone

    Use

    Pcr-based tagging

    Available Since

    Jan. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. PKC1-2GFP-ter-TRP1

    Plasmid

    #184773

    Purpose

    Integration of 2xGFP tag at PKC1 C terminus. Uses auxotrophic marker TRP1(Kluyveromyces lactis).

    Depositor

    Zhiping Xie

    Article

    Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

    Insert

    PKC1 (PKC1 Budding Yeast)
    

    Tags

    2EGFP

    Expression

    Yeast

    Available Since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pIALD2HMGr

    Plasmid

    #185867

    Purpose

    Knocking out ALD6; expressing acetylating aldehyde dehydrogenase (Lactobacillus reuteri pduP and L. reuteri EutE) and an NADH-preferring HMG-CoA reductase (Delftia acidovorans mvaA) in S. cerevisiae

    Depositor

    Claudia Vickers

    Article

    Hayat et al Microb Biotechnol. 2021 Nov;14(6):2627-2

    Insert

    ALD6(-125, 40)> PSk.GAL1>Da.mvaA>TPGK1-PADH1> Lr.pduP> TPDC1-PGAL2>Lr.EutE-ALD6(1054, 1749)
    

    Expression

    Yeast

    Mutation

    None

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. KBB430

    Plasmid

    #185091

    Purpose

    NMA111-GFP with first nuclear localization signal mutated (amino acids 28 - 30, KRK -> AAA)

    Depositor

    Ken Belanger

    Article

    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

    Insert

    NMA111
    

    Tags

    GFP

    Expression

    Yeast

    Mutation

    First nuclear localization signal mutated (amino …

    Available Since

    July 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. KBB438

    Plasmid

    #185092

    Purpose

    GAL1::Rfa1[1-337, 344-621]-GFP with NLS removed

    Depositor

    Ken Belanger

    Article

    Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

    Insert

    RFA1
    

    Expression

    Yeast

    Mutation

    Rfa1 with amino acids 338-343 deleted

    Promoter

    GAL1

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. KBB372

    Plasmid

    #185084

    Purpose

    GAL::Rfa1[330-621]-GFP under control of GAL1 promoter

    Depositor

    Ken Belanger

    Article

    Belanger et al DNA Cell Biol. 2011 Sep;30(9):641-51. do

    Insert

    RFA1
    

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Rfa1 amino acids 330-621

    Promoter

    GAL1

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. KBB428

    Plasmid

    #185090

    Purpose

    NMA111-GFP with first nuclear localization signal mutated (amino acids 9 - 11, KKR -> AAA)

    Depositor

    Ken Belanger

    Article

    Belanger et al J Cell Sci. 2009 Nov 1;122(Pt 21):3931-4

    Insert

    NMA111
    

    Tags

    GFP

    Expression

    Yeast

    Mutation

    First nuclear localization signal mutated (amino …

    Available Since

    July 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBY011-AMN1ko

    Plasmid

    #183099

    Purpose

    S. cerevisiae Sigma1278b AMN1 gene knock out plasmid with KanMX selection marker.

    Depositor

    Gabor Balazsi

    Article

    Guinn et al Commun Biol. 2022 Mar 31;5(1):292. doi:

    Insert

    AMN1 (left homology arm) - KanMX - AMN1 (right homology arm) (AMN1 Budding Yeast)
    

    Tags

    KanMX

    Expression

    Bacterial and Yeast

    Mutation

    only includes external sequences (504 bp each) as…

    Promoter

    URA3, TEF1

    Available Since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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