We narrowed to 11,647 results for: emb

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pCDNA4/TO-SPOP-myc (WT)

Plasmid

#197001

Purpose

Mammalian inducible expression construct of myc-tagged SPOP

Depositor

Tanja Mittag

Publication

Cuneo et al Mol Cell. 2023 Mar 2;83(5):731-745.e4. d

Insert

SPOP (Spop Mouse)

Tags

myc

Expression

Mammalian

Mutation

-

Available since

April 4, 2023

Availability

Academic Institutions and Nonprofits only

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pnEA-NpG-HsNot2_429-540_T

Plasmid

#147617

Purpose

Bacterial Expression of HsNot2_429-540

Depositor

Elisa Izaurralde

Publication

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot2_429-540 (CNOT2 Human)

Expression

Bacterial

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot7-C67EL71E_S

Plasmid

#147509

Purpose

Mammalian Expression of HsNot7-C67EL71E

Depositor

Elisa Izaurralde

Publication

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot7-C67EL71E (CNOT7 Human)

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot3_1-241_K

Plasmid

#146801

Purpose

Mammalian Expression of HsNot3_1-241

Depositor

Elisa Izaurralde

Publication

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot3_1-241 (CNOT3 Human)

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsNot3_589-753_K

Plasmid

#146803

Purpose

Mammalian Expression of HsNot3_589-753

Depositor

Elisa Izaurralde

Publication

Boland et al Nat Struct Mol Biol. 2013 Nov;20(11):128

Insert

HsNot3_589-753 (CNOT3 Human)

Expression

Mammalian

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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OM14-2EGFP-UG74-ter

Plasmid

#184758

Purpose

Marker for yeast mitochondria. Integration of 2xGFP tag at OM14 C terminus. Uses antibiotic resistance marker natMX6.

Depositor

Zhiping Xie

Publication

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

OM14 (OM14 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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SEC7-2EGFP-ter-NAT

Plasmid

#184764

Purpose

Marker for yeast late Golgi/early endosome . Integration of 2xGFP tag at SEC7 C terminus. Uses antibiotic resistance marker natMX6.

Depositor

Zhiping Xie

Publication

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

SEC7 (SEC7 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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ACH1-2EGFP-ter-URA3

Plasmid

#184769

Purpose

Marker for yeast mitochondria. Integration of 2xGFP tag at ACH1 C terminus. Uses auxotrophic marker URA3(Candida albicans).

Depositor

Zhiping Xie

Publication

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

ACH1 (ACH1 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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SYS1-2EGFP-ter-URA3

Plasmid

#184771

Purpose

Marker for yeast early Golgi. Integration of 2xGFP tag at SYS1 C terminus. Uses auxotrophic marker URA3(Candida albicans).

Depositor

Zhiping Xie

Publication

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

SYS1 (SYS1 Budding Yeast)

Tags

2EGFP

Expression

Yeast

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn-iEscSnFR_ER

Plasmid

#182818

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_ER

Use

AAV

Promoter

hSyn

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn-iEscSnFR_PM

Plasmid

#182819

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_PM

Use

AAV

Promoter

hSyn

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn-iEscSnFR_cyto

Plasmid

#182820

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iEscSnFR_cyto

Use

AAV

Promoter

hSyn

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn-iFluoxSnFR_ER

Plasmid

#182821

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iFluoxSnFR_ER

Use

AAV

Promoter

hSyn

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn-iFluoxSnFR_PM

Plasmid

#182822

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iFluoxSnFR_PM

Use

AAV

Promoter

hSyn

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV9-hSyn-iFluoxSnFR_cyto

Plasmid

#182823

Purpose

Develop a family of genetically encoded fluorescent biosensors for nicotinic agonists, termed iDrugSnFRs, thus enabling optical subcellular pharmacokinetics for nicotinic agonists

Depositor

Henry Lester

Publication

Nichols et al J Neurosci. 2023 Mar 2:JN-RM-1519-22. do

Insert

iFluoxSnFR_cyto

Use

AAV

Promoter

hSyn

Available since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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ppwd1.473.646.W601A

Plasmid

#137660

Purpose

Expresses N-terminal (His)6-Thrombin cleavage site fusion of human gene or portion of gene with point mutation in bacterial strains. pET based vector.

Depositor

Tara Davis, Melissa Jurica

Publication

Adams et al Biochem J. 2015 Jul 15;469(2):223-33. do

Insert

ppwd1 (PPWD1 Human)

Tags

(His)6-thrombin

Expression

Bacterial

Mutation

473-646-W601A

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pClgn1.1_Dcst1-3xHA

Plasmid

#183540

Purpose

Express Dcst1-3xHA under the mouse Clgn promoter.

Depositor

Masahito Ikawa

Publication

Noda et al Commun Biol. 2022 Apr 7;5(1):332. doi: 1

Insert

DC-STAMP domain containing 1 (Dcst1 Mouse)

Tags

HA

Expression

Mammalian

Available since

July 29, 2022

Availability

Academic Institutions and Nonprofits only

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pClgn1.1_Dcst2-3xHA

Plasmid

#183541

Purpose

Express Dcst2-3xHA under the mouse Clgn promoter.

Depositor

Masahito Ikawa

Publication

Noda et al Commun Biol. 2022 Apr 7;5(1):332. doi: 1

Insert

DC-STAMP domain containing 2 (Dcst2 Mouse)

Tags

HA

Expression

Mammalian

Available since

May 24, 2022

Availability

Academic Institutions and Nonprofits only

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pMSP1D12W/2Y

Plasmid

#124275

Purpose

Study of protein insertion into nanodisks by means of changes in tryptophan fluorescence

Depositor

Alexey S. Ladokhin

Insert

Membrane scaffold protein

Tags

7xHis-tag

Expression

Bacterial

Mutation

Two tryptophans changed to two tyrosines

Promoter

T7

Available since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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C232-E08: attB2r-mTFP1-attB3

Plasmid

#162904

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal mTFP1 fusion proteins

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Teal fluorescent protein

Use

Synthetic Biology

Available since

Nov. 11, 2021

Availability

Academic Institutions and Nonprofits only

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