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pBN080

Plasmid

#167961

Purpose

express E. coli E69 (O9a:K30) Wzc with Y717E, Y718E

Depositor

James Naismith

Publication

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y717E, Y718E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y717E, Y718E

Promoter

araBAD

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pBN074

Plasmid

#167955

Purpose

express E. coli E69 (O9a:K30) Wzc with Y705E, Y706E

Depositor

James Naismith

Publication

Yang et al Nat Commun. 2021 Jul 16;12(1):4349. doi:

Insert

E. coli E69 (O9a:K30) Wzc with Y705E, Y706E

Tags

hexa-histidine tag

Expression

Bacterial

Mutation

Y705E, Y706E

Promoter

araBAD

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnj6

Plasmid

#173133

Purpose

This gene was cloned into the gateway middle entry vector. It can be used for generating antisense riboprobe or gene expression using a gateway system.

Depositor

GuangJun Zhang

Publication

Silic et al Dev Dyn. 2021 Sep 23. doi: 10.1002/dvdy.

Insert

kcnj6 (kcnj6 Zebrafish)

Use

Pcr cloning vector

Promoter

No promoter at 5 end; T7 promoter at 3 end.

Available since

Sept. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pGEMHE-bNup98-DN-NLS-mClover3

Plasmid

#171495

Purpose

T7 promotor drives in vitro transcription of mClover3-tagged bovine Nup98 DN mRNA

Depositor

Melina Schuh

Publication

Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

Insert

Nup98-DN (NUP98 B. tarus (bovine))

Use

Pgehme

Tags

mClover3

Promoter

T7

Available since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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C232-E06: attB2r-mCherry-attB3

Plasmid

#162903

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal mCherry fusion proteins

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Cherry fluorescent protein

Use

Synthetic Biology

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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C453-E17: attB4-PGKp>-attB5

Plasmid

#162976

Purpose

Gateway attB4/attB5 entry clone for mammalian expression using the human PGK promoter

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human pGK promoter

Use

Synthetic Biology

Available since

May 5, 2021

Availability

Academic Institutions and Nonprofits only

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C232-E25: attB2r-mVenus-attB3

Plasmid

#162905

Purpose

Gateway attB2r/attB3 entry clone for construction of C-terminal mVenus fusion proteins

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

monomeric Venus fluorescent protein

Use

Synthetic Biology

Available since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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C413-E21: attB4-PGKp>-attB1r

Plasmid

#162921

Purpose

Gateway attB4/attB1r entry clone for mammalian expression using the human PGK promoter

Depositor

Dominic Esposito

Publication

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Insert

human pGK promoter

Use

Synthetic Biology

Available since

April 15, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221-kcnt1a

Plasmid

#164961

Purpose

Zebrafish kcnt1a gene CDS

Depositor

GuangJun Zhang

Publication

Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

Insert

kcnt1a (kcnt1 Zebrafish)

Use

Gateway Cloning

Available since

March 18, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB2lock

Plasmid

#160530

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Publication

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB2lock

Tags

His-Tag

Expression

Bacterial

Mutation

Y208C, D322C

Available since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-HB1lock

Plasmid

#160523

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Publication

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-HB1lock

Tags

His-Tag

Expression

Bacterial

Mutation

A221C, N404C

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pTricHis-ILY-WT-charge+

Plasmid

#160513

Purpose

Expression of inserted ILY variant

Depositor

Doryen Bubeck

Publication

Shah et al Nat Commun. 2020 Nov 16;11(1):5818. doi:

Insert

ILY-WT-charge+

Tags

His-Tag

Expression

Bacterial

Mutation

Y336K, L340K

Available since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pTV-Dnmt3l-tdiRFP670-PEST

Plasmid

#122358

Purpose

Targeting vector for mouse Dnmt3l to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Publication

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Dnmt3l

Use

Mouse Targeting

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Etv5-tdiRFP670-PEST

Plasmid

#122359

Purpose

Targeting vector for mouse Etv5 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Publication

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Etv5

Use

Mouse Targeting

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Jade1-tdiRFP670-PEST

Plasmid

#122360

Purpose

Targeting vector for mouse Jade1 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Publication

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Jade1

Use

Mouse Targeting

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Kdm1a-tdiRFP670-PEST

Plasmid

#122361

Purpose

Targeting vector for mouse Kdm1a to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Publication

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Kdm1a

Use

Mouse Targeting

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Ncapd3-tdiRFP670-PEST

Plasmid

#122362

Purpose

Targeting vector for mouse Ncapd3 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Publication

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Ncapd3

Use

Mouse Targeting

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Npm1-tdiRFP670-PEST

Plasmid

#122363

Purpose

Targeting vector for mouse Npm1 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Publication

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Npm1

Use

Mouse Targeting

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Dnmt3b-tdiRFP670-PEST

Plasmid

#122364

Purpose

Targeting vector for mouse Dnmt3b to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Publication

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Dnmt3b

Use

Mouse Targeting

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pTV-Dhx15-tdiRFP670-PEST

Plasmid

#122365

Purpose

Targeting vector for mouse Dhx15 to insert 2A-tdiRFP670-PEST into immediately upstream of stop codon.

Depositor

Hiroshi Ochiai

Publication

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

2A-tdiRFP-PEST with homology arms for Dhx15

Use

Mouse Targeting

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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