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2XSmBiT-SARS-CoV-2 S1

Plasmid

#173430

Purpose

protein expression plasmid of 2XSmBiT-SARS-CoV-2 S1-IgG1 Fc

Depositor

David Nemazee

Publication

Huang et al J Immunol. 2021 Jul 1;207(1):344-351. do

Insert

2XSmBiT SARS-CoV-2 S1-IgG1 Fc (S SARS-CoV-2)

Use

Luciferase

Tags

human IgG1 Fc

Expression

Mammalian

Mutation

furin cleavage site RRAR to GSAS

Promoter

CMV

Available since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-LMO-IRES-EGFP

Plasmid

#178309

Purpose

Express the bacterial lactate 2-monooxygenase (LMO) enzyme in mammalian cells

Depositor

Sergey Kasparov

Publication

Vaccari Cardoso et al Brain Sci. 2021 Aug 10;11(8). pii: brain

Insert

Lactate 2-monooxygenase

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Jan. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1a-Nla-cas5

Plasmid

#178878

Purpose

Expression of human codon-optimized Nlacas5 with C terminal NLS and HA tag

Depositor

Yan Zhang

Publication

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas5c-bGH polyA

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a

Available since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1a-Nla-cas7

Plasmid

#178879

Purpose

Expression of human codon-optimized Nlacas7 with C terminal NLS and HA tag

Depositor

Yan Zhang

Publication

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas7c-bGH PolyA

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a

Available since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1a-Nla-cas8

Plasmid

#178880

Purpose

Expression of human codon-optimized NlaCas8 with N terminal NLS and HA tag

Depositor

Yan Zhang

Publication

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas8c-bGH PolyA

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a

Available since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pSmart-EF1a-Nla-cas11

Plasmid

#178882

Purpose

Expression of human codon-optimized NlaCas11 with N terminal NLS and HA tag

Depositor

Yan Zhang

Publication

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

EF1a-NlaCas11c-bGH polyA

Tags

HA and NLS

Expression

Mammalian

Promoter

EF1a

Available since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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LT2_7_OxPro

Plasmid

#177709

Purpose

Plasmid for genome integration in X2 site expressing OxPro (oxidative stress biosensor)

Depositor

Lisbeth Olsson

Publication

Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

Inserts

pTRX2_5xUAS-ymYPET-tCYC1
pTEFmut8-mCherry-tADH1

Use

Used as donor dna in genome integration after lin…

Expression

Yeast

Available since

Jan. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBacMam4_N_Flag_His8_eGFP_TEV

Plasmid

#178808

Purpose

Baculovirus gene transfer into Mammalian cells to product recombinant proteins.

Depositor

Kazuhiro Abe

Type

Empty backbone

Tags

Flag, His8, eGFP, TEV

Expression

Mammalian

Promoter

CMV

Available since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pRibo-APEX2m

Plasmid

#176842

Purpose

Expression of APEX2 from a codon-optimized gene (multi-copy episomal plasmid)

Depositor

Jessica Seeliger

Publication

Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

Insert

pRibo-APEX2m

Expression

Bacterial

Promoter

pRibo, theophilline riboswitch inducible hsp60 pr…

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRibo-Sec-APEX2m

Plasmid

#176844

Purpose

Expression of APEX2 in the periplasm from a codon-optimized gene (multi-copy episomal plasmid)

Depositor

Jessica Seeliger

Publication

Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

Insert

pRibo-Sec-APEX2m

Use

Inducible

Expression

Bacterial

Promoter

pRibo, theophilline riboswitch inducible hsp60 pr…

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pRiboI-Sec-APEX2m

Plasmid

#176845

Purpose

Expression of APEX2 in the periplasm from a codon-optimized gene (single-copy integrating plasmid)

Depositor

Jessica Seeliger

Publication

Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

Insert

pRibo-Sec-APEX2m

Expression

Bacterial

Promoter

pRibo, theophilline riboswitch inducible hsp60 pr…

Available since

Jan. 10, 2022

Availability

Academic Institutions and Nonprofits only

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Gi-c010

Plasmid

#173080

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Publication

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

GAP

Tags

mCitrine

Expression

Bacterial

Mutation

mCitrine + last 10 residues of GNA1, codon optimi…

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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RNF146-c005

Plasmid

#173091

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Publication

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

RNF146 (RNF146 Human)

Tags

mCerulean

Expression

Bacterial

Mutation

R99-L183, codon optimized

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pGPTVII-nlsSED1

Plasmid

#178190

Purpose

SED1 biosensor for expression in Arabidopsis thaliana

Depositor

Cesar Cuevas-Velazquez, Jose Dinneny

Publication

Cuevas-Velazquez et al Nat Commun. 2021 Sep 14;12(1):5438. doi:

Insert

SED1 biosensor

Tags

SV40 NLS

Expression

Plant

Promoter

pUBQ10

Available since

Dec. 9, 2021

Availability

Academic Institutions and Nonprofits only

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ARH1-c003

Plasmid

#173093

Purpose

Expression of recombinant fusion protein in E. coli

Depositor

Lari Lehtiö

Publication

Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

Insert

ADPRH (ADPRH Human)

Tags

mCerulean

Expression

Bacterial

Mutation

E2-L357, codon optimized

Available since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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UIMLx2

Plasmid

#174888

Purpose

UIMLx2 ubiquitin probe for K48 ubiquitylated proteins

Depositor

Peter Kaiser

Publication

Villamil et al Mol Cell Proteomics. 2022 Jan;21(1):1001

Insert

Met4 UIML

Tags

Avi-6xHis-Smt3

Expression

Bacterial

Available since

Nov. 10, 2021

Availability

Academic Institutions and Nonprofits only

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EC2_3_dCas9_Mxi1_sgRNA

Plasmid

#163708

Purpose

Yeast low copy plasmid with dCas9, sgRNA expression cassette and Mxi1 transcriptional repressor

Depositor

Yvonne Nygård

Publication

Camara et al Sci Rep. 2020 Sep 3;10(1):14605. doi: 10

Inserts

dCas9
Mxi1+SV40 NLS

Expression

Yeast

Mutation

Point mutations D10A and H840A

Promoter

ScTEF1

Available since

Nov. 1, 2021

Availability

Academic Institutions and Nonprofits only

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SspB-iRFP-DHPH-TIAM1

Plasmid

#176118

Purpose

Heterodimerization with iLID, Rac1 activation

Depositor

Dorus Gadella

Publication

Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

Insert

TIAM1 (TIAM1 Human)

Expression

Mammalian

Available since

Oct. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pETSXM2-pLacI-mtrC

Plasmid

#174615

Purpose

Bacterial expression of mtrC from Shewanella oneidensis MR-1.

Depositor

I-Son Ng

Publication

I-Son Ng et al Bioresour. Bioprocess. 5, 35 (2018)

Insert

MtrC

Use

Synthetic Biology; Shuttle vector in e. coli and …

Expression

Bacterial

Promoter

pLacI

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pAT9752-BEAR-mScarlet

Plasmid

#162991

Purpose

BEAR target plasmid with split mScarlet and disrupted 5' splice site

Depositor

Ervin Welker

Publication

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

mScarlet split with an intron between amino acids 110-111

Use

CRISPR

Expression

Mammalian

Mutation

disrupted 5' splice site

Promoter

CMV

Available since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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