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We narrowed to 79 results for: kan

Showing: 1 - 20 of 79 results

  1. Plasmids 101: Choosing an Antibiotic Resistance Gene

    Type
    Blog Post
    ....   Kanamycin Kanamycin, or kan, is an aminoglycoside which inhibits protein synthesis. The kan resistance...resistance gene, NPTII, facilitates phosphorylation of kan, which inactivates it. The NPTII gene confers resistance...decorative" title="Check icon" %} Satellite colonies Kanamycin Excellent No Bacteria and eukaryotes (G418) {%...

  3. Bacterial Expression Systems

    Type
    Collection
    ...Methylobacterium extorquens Julia Vorholt 84693 pMyNT-kan Acetamidase promoter Acetamide Escherichia coli ,...Mycobacterium smegmatis Matthias Wilmanns 84692 pMyC-kan Acetamidase promoter Acetamide Escherichia coli ,...Mycobacterium smegmatis Matthias Wilmanns 84689 pMyBADC-kan pBAD Arabinose Escherichia coli , Mycobacterium smegmatis...) Escherichia coli Cynthia Collins 84689 pMyBADC-kan pBAD Arabinose Escherichia coli , Mycobacterium smegmatis...

  4. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...previously described pFA6a-link vectors and include a Kan, SpHIS5, or CaURA3 selection marker. Lee et al.(1...3xFlag, Protein A, or V5), and selection marker (KanMX, HphMX, or His3MX).  Each PCR product from this ...

  6. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...instead use G418. This can be confusing since the neo/kan gene confers G418 resistance; however, like gentamicin...

  8. CRISPR Plasmids - Plants

    Type
    Collection
    ... Yang 62202 pKSE401 AtU6-26 yes, cut S. pyogenes Kan Chen 50929 pRGE31 rice snoRNA U3 BsaI none S. pyogenes...

  9. Evolution of Lab Techniques

    Type
    Blog Post
    ...PubMed PMID: 1195397. 2. Chang, Judy C., and Yuet Wai Kan. "A sensitive new prenatal test for sickle-cell anemia...

  10. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Clover - Mammalian Expression pFA6a-link-yoClover-Kan - Yeast Expression dClover2 505 515 Dimer (A206K)...C1 - Mammalian Expression pFA6a-link-yoTagRFP657-Kan - Yeast Expression smURFP + BV 642 670 32 39 min ...Bacterial Expression tandem PA-GFP-KanR - Yeast Expression pFA6a-PA-GFP-kanMX6 - Yeast Expression pFA6a-link-yEPAGFP-CaUra3...

  11. CRISPR Plasmids - Empty gRNA Vectors

    Type
    Collection
    ...Basta Chen pKSE401 62202 Plant yes, cut S. pyogenes Kan Chen pHUE411 62203 Plant yes, cut S. pyogenes Hyg...Jackson pMZ376 74213 Yeast rrk1 yes, cut S. pyogenes KanMX Zaratiegui pMZ377 74214 Yeast rrk1 yes, cut S. pyogenes...

  12. Cre-lox system

    Type
    Collection
    ...Cre rTH AAV Wilson 108454 mCherry-p2A-CreERT2-FRT-kan-FRT For in vitro transcription of Cre or to recombine...; Targeting vector for Sox1 locus CAG Mammalian Okano 153207 pAM-AAV-mSncg-Cre Retinal ganglion cell-specific...

  13. Viral Vectors 101: AAV Serotypes and Tissue Tropism

    Type
    Blog Post
    ...frequencies in the central nervous system (CNS) (Wu, Asokan, et al., 2006). AAV1 has also been found to transduce...kidney, and retinal cells (Issa et al., 2023; Wu, Asokan, et al., 2006). AAV2 capsids are available as plasmids...kidney, and retinal cells (Issa et al., 2023; Wu, Asokan, et al., 2006). AAV8 capsids are available as plasmids...varies by species and route of administration (Wu, Asokan, et al., 2006). Want to know if a capsid is a good...doi.org/10.2337/diabetes.55.04.06.db05-0927 Wu, Z., Asokan, A., & Samulski, R. J. (2006). Adeno-associated...

  14. Hot Plasmids - September 2019 - CRISPR, Auxin Inducible Degradation, MerMAID Channelrhodopsins, and Genome Looping

    Type
    Blog Post
    ...with the cells’ endogenous ubiquitin ligase, the Kanemaki lab was able to translate AID to yeast, as well...Nishimura et al., 2009, Natsume et al., 2016).  The Kanemaki lab previously developed a CRISPR/Cas9 based method...essential proteins (Natsume et al., 2016). Now, the Kanemaki lab has created a new toolkit of plasmids for ...create conditional human cell lines. Lastly, the Kanemaki lab demonstrated that auxinole, a TIR1 inhibitor...

  15. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    ...serotype can affect spread from the injection site  (Kanaan et al., 2017), axonal transport  (Murlidharan et...cells12050785 Murlidharan, G., Samulski, R. J., & Asokan, A. (2014). Biology of adeno-associated viral vectors...5), 1–41. https://doi.org/10.3390/cells12050785 Kanaan, N. M., Sellnow, R. C., Boye, S. L., Coberly, B...pharmaceutics13050750 Murlidharan, G., Samulski, R. J., & Asokan, A. (2014). Biology of adeno-associated viral vectors...

  16. Illuminating Choices: A Guide to Selecting Fluorescent Dyes and Ligands

    Type
    Blog Post
    ...signals when used with the same protein (Hayashi-Takanaka et al., 2014). Other dyes may prove challenging...and reliable imaging and assay results (Hayashi-Takanaka et al., 2014)​​. Conjugates with lower DOL tend...intracellular fluorescence live imaging ​​(Hayashi-Takanaka et al., 2014). Dyes can also vary in their brightness...994. https://doi.org/10.1038/nmeth.4403 Hayashi-Takanaka, Y., Stasevich, T. J., Kurumizaka, H., Nozaki,...

  18. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    ...activity after ischemic brain injury Dr. Gohar Tsakanova, Institute of Molecular Biology of the NationalAcademy...Sciences of the Republic of Armenia, Armenia Gohar Tsakanova is a Senior Researcher who investigates the molecular...two-photon laser scanning microscope will allow Tsakanova to image cortical neurons and study changes in...

  19. Viral Vectors 101: The Retroviral Lifecycle

    Type
    Blog Post
    ...Structure of a retrovirus. Image re-used from Dong & Kantor, 2021 under CC-BY license. Figure 2 gives a...1406488. PubMed Central PMCID: PMC372876 Dong, W., & Kantor, B. (2021). Lentiviral Vectors for Delivery of ...PubMed Central PMCID: PMC4515362 Robinson, W. S., Pitkanen, A., & Rubin, H. (1965). The nucleic acid of the...

  20. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ... based on a bacterial haloalkane dehalogenase that binds a synthetic chloralkane (CA) ligand. The current...  (TMP-tag) Few Easy HaloTag 295 Chloralkane (CA) Many Easy CLIP-tag 182 Benzylcytosine...
Showing: 1 - 20 of 79 results