Zinc Finger Consortium: OPEN Reagents
Depositing Labs: Keith Joung
This kit consists of plasmids and strains used to engineer ZFNs via the Oligomerized Pool ENgineering (OPEN) method.
This kit consists of 5 individually packaged plasmids and 3 individually packaged strains, which are shipped as bacterial stabs at room temperature. Individual plasmids can be ordered from each plasmid page and will be shipped as bacterial stabs.
Original PublicationOligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays. Maeder ML, Thibodeau-Beganny S, Sander JD, Voytas DF, Joung JK. Nature Protocols . 2009;4(10):1471-501. doi: 10.1038/nprot.2009.98. Epub 2009 Sep 17. PubMed.
Use of the OPEN method requires OPEN pools (available by request from the Joung lab) and the following reagents (listed below and available individually or as an “OPEN Accessory Reagent Set”). These reagents are also valuable research tools for using the bacterial two-hybrid system originally described by Hochschild and colleagues ( Dove et al., Nature 1997) and later modified by Pabo and colleagues ( Joung et al., PNAS 2000).
The Oligomerized Pool ENgineering (OPEN) method has been used to engineer ZFNs that function in zebrafish, plants, and human cells ( Maeder et al., Mol Cell 2008, Foley & Yeh et al., PLoS ONE 2009, Townsend et al., Nature 2009, Zou et al., Cell Stem Cell 2009).
A detailed protocol for practicing OPEN is available in the 2009 Joung Nature Protocols paper.
How to Cite this Kit
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.
For your Materials and Methods section:
"The plasmid kit used in this publication was a gift from Keith Joung (Addgene kit # 1000000013)"
For your Reference section:
Oligomerized pool engineering (OPEN): an 'open-source' protocol for making customized zinc-finger arrays. Maeder ML, Thibodeau-Beganny S, Sander JD, Voytas DF, Joung JK. Nature Protocols . 2009;4(10):1471-501. doi: 10.1038/nprot.2009.98. Epub 2009 Sep 17. PubMed PMID 19798082.