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pGL3-noSV40-humanCOL2A1enhancer

Plasmid

#173961

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Enhancer region from COL2A1 gene

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-ABE8e

Plasmid

#193645

Purpose

Expression of base editor dCas12a-ABE8e in mammalian cells for base editing, along with non-fused EGFP fluorescence

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-TadA*8e, EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pSW031

Plasmid

#231144

Purpose

Used to express cyclic sortase cW11 (recognizes APXTG motif) from E. coli.

Depositor

Philip Cole

Article

Whedon et al J Am Chem Soc. 2024 Nov 25. doi: 10.1021

Insert

S. aureus Sortase A mutant

Tags

Nostoc punctiform split intein C-terminal fragmen…

Expression

Bacterial

Mutation

P94S, E108Q, S116V, D160N, T164Q, V168M, L169H, D…

Promoter

T7

Available Since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-GRAB-rDA2h

Plasmid

#208701

Purpose

Expresses the genetically-encoded fluorescent dopamine (DA) sensor GRAB_rDA2h in neurons

Depositor

Yulong Li

Article

Zhuo et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

Insert

GPCR activation based dopamine (DA) sensor GRAB_rDA2h

Use

AAV

Promoter

hSyn

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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lentiCRISPR v2-Nr4a1 double gRNAs

Plasmid

#162791

Purpose

Multiplex Guide RNAs to generate Nr4a1 knockout by CRISPR

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Multiplex Nr4a1 gRNA 1 and 2 (Nr4a1 Mouse)

Use

CRISPR and Lentiviral

Promoter

hU6

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pCC1-4k-BBclone2

Plasmid

#137070

Purpose

E. coli plasmid that contains the expression optimized genes for BB0323, P13, DipA and Lmp1

Depositor

Debra Hansen

Article

Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

Inserts

BB0323
P13
DipA
Lmp1

Expression

Bacterial

Mutation

wild-type, full-length (signal-peptide-containing…

Available Since

May 18, 2020

Availability

Academic Institutions and Nonprofits only

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TRBV7-9

Plasmid

#123437

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV7-9 (TRBV7-9 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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TRAV26-1

Plasmid

#123398

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRAV26-1 (TRAV26-1 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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ρ1-EM GABAA receptor in pEZT-BM

Plasmid

#213072

Purpose

Express human GABA-A rho1 receptor with truncated intracellular domain and superfolder GFP insertion in ICD

Depositor

Erik Lindahl

Article

Cowgill et al Neuron. 2023 Nov 1;111(21):3450-3464.e5.

Insert

Human GABAa rho1 receptor (GABRR1 Human)

Use

Bacmam, baculovirus, oocyte expression

Tags

Twin-Strep tag and superfolderGFP

Expression

Mammalian

Mutation

Encodes residues S58–L383 and D451–S479, separate…

Promoter

CMV and T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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TRAV26-2

Plasmid

#123399

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRAV26-2 (TRAV26-2 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap-FLEX.SF-iGluSnFR.A184S

Plasmid

#106180

Purpose

Tight affinity glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

Depositor

Loren Looger

Article

Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

Has Service

AAV5

Insert

SF-iGluSnFR.A184S

Use

AAV

Mutation

GltI: A184S

Promoter

hSynapsin

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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pB-Halo,IRES-NGFR

Plasmid

#164520

Purpose

All-in-One piggyBac transposon Gateway Destination vector for dox-inducible expression of N-terminal Halo tagged protein (inducible NGFR and constitutive rtTA and neomycin resistance)

Depositor

Mitchell Guttman

Article

Banerjee et al Cell. 2020 Nov 25;183(5):1325-1339.e21.

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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TRBV19

Plasmid

#123453

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV19 (TRBV19 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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H3S10ph biosensor

Plasmid

#120809

Purpose

FRET biosensor. To monitor histone H3 Serine 10 phosphorylation in mammalian cells by FRET

Depositor

Yingxiao Wang

Article

Peng et al Proc Natl Acad Sci U S A. 2018 Nov 26. p

Insert

Mouse histone H3-CFP-FHA2-histone H3 peptide (1-14aa)-YFP

Expression

Mammalian

Mutation

Threonine 3, Threonine 6, and Threonine 11 are mu…

Promoter

CMV

Available Since

Feb. 12, 2019

Availability

Academic Institutions and Nonprofits only

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TRBV5-1

Plasmid

#123414

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV5-1 (TRBV5-1 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 10, 2019

Availability

Academic Institutions and Nonprofits only

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5_T7-GAP43-mScarlet3

Plasmid

#225932

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the GAP43 membrane localisation signal

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv [Preprint]. 2025 Nov 29:2024.11.

Insert

GAP43-mScarlet3

Use

In vitro transcription

Promoter

T7

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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4_T7-mScarlet3-RitC

Plasmid

#225931

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the H-RitC membrane localisation signal

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv [Preprint]. 2025 Nov 29:2024.11.

Insert

mScarlet3-RitC

Use

In vitro transcription

Promoter

T7

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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TRBV20-1

Plasmid

#123454

Purpose

construction of TCR

Depositor

Catherine Wu

Article

Hu et al Blood. 2018 Nov 1;132(18):1911-1921. doi

Insert

TRBV20-1 (TRBV20-1 Human)

Expression

Bacterial

Mutation

WT

Available Since

May 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-dCas12a-A3A-Y132D

Plasmid

#193641

Purpose

Expression of base editor dCas12a-A3A-Y132D in mammalian cells for base editing

Depositor

Liangxue Lai

Article

Chen et al Commun Biol. 2022 Nov 2;5(1):1163. doi:

Insert

dLbCas12a-hA3A-Y132D

Expression

Mammalian

Mutation

hAPOBEC3A-Y132D

Promoter

CMV

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pLVX-Phi3-AURKB-PGK-mCherry

Plasmid

#153316

Purpose

Expresses AURKB and mCherry in mammalian cells.

Depositor

Robert Judson-Torres

Article

McNeal et al Elife. 2021 Nov 23;10. pii: 70385. doi:

Insert

AURKB (AURKB Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 30, 2022

Availability

Academic Institutions and Nonprofits only

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