Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 10,047 results for: pET

Showing: 9861 - 9880 of 10047 results

Results per page:

Sort results by:

His-Thrombin-Munc13-1 C1-C2B-MUN-C2C (aa529-1735 delta1408-1452 delta1533-1551)

Plasmid

#243821

Purpose

Munc13-1 protein purification

Depositor

Jose Rizo-Rey

Publication

Quade et al Elife. 2019 Feb 28;8:e42806. doi: 10.755

Insert

Munc13-1 (Unc13a Rat)

Tags

His

Expression

Bacterial

Mutation

delta1408-1452 delta1533-1551

Available since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Methyl-binding domain protein 2 (MBD2) coiled-coil domain

Plasmid

#132721

Purpose

Bacterial expression vector for methyl-binding domain protein 2 (MBD2) coiled-coil domain for use as competitor peptide in measurements of effective concentrations

Depositor

Magnus Kjaergaard

Publication

Sorensen et al Methods Mol Biol. 2020;2141:505-518. doi

Insert

Methyl-binding protein domain 2 coiled-coil domain

Tags

N-terminal His-tag. Flanking linkers to match acc…

Expression

Bacterial

Promoter

T/

Available since

Oct. 8, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
His-Thrombin-Munc13-1 C1-C2B-MUN-C2C R1598E F1658E (delta1408-1452 delta1533-1551)

Plasmid

#243822

Purpose

Munc13-1 protein purification

Depositor

Jose Rizo-Rey

Publication

Quade et al Elife. 2019 Feb 28;8:e42806. doi: 10.755

Insert

Munc13-1 (Unc13a Rat)

Tags

His

Expression

Bacterial

Mutation

delta1408-1452 delta1533-1551 R1598E F1658E

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG8Ha-FLI1_DBD_R2L2

Plasmid

#188049

Purpose

Expresses His8-FLI1_DBD_R2L2(276-399_R337L_R340L_F362A) with a TEV site for removal of the His8 tag.

Depositor

David Libich

Publication

Selig et al Proc Natl Acad Sci U S A. 2025 May 20;12

Insert

FLI1 (FLI1 Human)

Tags

His8

Expression

Bacterial

Mutation

deleted amino acids 1-275, 400-452, mutated R337L…

Promoter

T7

Available since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG415Gal_Gcn4 E3GFP

Plasmid

#233008

Purpose

Galactose iduced expression of Gcn4 E+GFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 E+

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Mutation

D105E, D118E, D139E

Promoter

Gal1

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG415Gal_Gcn4 Aro2+

Plasmid

#232982

Purpose

Galactose iduced expression of Gcn4 Aro2+ in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro2+

Tags

TEV cleavage site

Expression

Yeast

Mutation

N116F, S117E, E119N, P129Q, T132Y

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG415Gal_Gcn4 Aro3+

Plasmid

#232983

Purpose

Galactose iduced expression of Gcn4 Aro3+ in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro3+

Tags

TEV cleavage site

Expression

Yeast

Mutation

D103Y, N112T, L113Y, S117F, K140R

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG415Gal_Gcn4 Aro3+HA

Plasmid

#232990

Purpose

Galactose iduced expression of Gcn4 Aro3+HA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro3+

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

D103Y, N112T, L113Y, S117F, K140R

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG415Gal_Gcn4 Aro2+HA

Plasmid

#232989

Purpose

Galactose iduced expression of Gcn4 Aro2+HA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 Aro2+

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

N116F, S117E, E119N, P129Q, T132Y

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG415Gal_Gcn4 F124IGFP

Plasmid

#233005

Purpose

Galactose iduced expression of Gcn4 F124IGFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 F124I

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Mutation

F124I

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG415Gal_Gcn4 WW+HA

Plasmid

#232991

Purpose

Galactose iduced expression of Gcn4 WW+HA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WW+

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

E109N, S117D, T121W, N126W, T132W

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG415Gal_Gcn4 WW+GFP

Plasmid

#233003

Purpose

Galactose iduced expression of Gcn4 WW+GFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 WW+

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Mutation

E109N, S117D, T121W, N126W, T132W

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG415Gal_Gcn4 W120AGFP

Plasmid

#233004

Purpose

Galactose iduced expression of Gcn4 120AGFP in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 W120A

Tags

HA-GFP-HA and TEV cleavage site

Expression

Yeast

Mutation

W120A

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG415Gal_Gcn4 S/ArotoL

Plasmid

#232979

Purpose

Galactose iduced expression of Gcn4 S/ArotoL in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 S/ArotoL

Tags

TEV cleavage site

Expression

Yeast

Mutation

S101L, S104L, F108L, Y110L, S117L, S136L, S144L

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
ppAG415Gal_Gcn4 L113A+

Plasmid

#232972

Purpose

Galactose iduced expression of Gcn4 L113A in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 L113A

Tags

TEV cleavage site

Expression

Yeast

Mutation

L113A, E114Y, N126H, D127E, S136A

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG415Gal_Gcn4 ILVtoED

Plasmid

#232957

Purpose

Galactose iduced expression of Gcn4 ILVtoED in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoED

Tags

TEV cleavage site

Expression

Yeast

Mutation

L123D, I128E, V130E, V135E, L137D, I142D

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG415Gal_Gcn4 R3W

Plasmid

#232975

Purpose

Galactose iduced expression of Gcn4 R++ W+ in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 R++ W+

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

D103R, N126W, D133R, K143R

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG415Gal_Gcn4 E3HA

Plasmid

#232996

Purpose

Galactose iduced expression of Gcn4 E+HA in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 E+

Tags

3xHA and TEV cleavage site

Expression

Yeast

Mutation

D105E, D118E, D139E

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG415Gal_Gcn4 ILVtoAS

Plasmid

#232959

Purpose

Galactose iduced expression of Gcn4 ILVtoAS in yeast

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 ILVtoAS

Tags

TEV cleavage site

Expression

Yeast

Mutation

L123S, I128S, V130A, V135A, L137S, I142S

Promoter

Gal1

Available since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pDest17_Gcn4 SATtoG KtoR

Plasmid

#231861

Purpose

Bacterial expression of N-terminally 6His tagged Gcn4 SATtoG KtoR

Depositor

Aseem Ansari, Tanja Mittag

Publication

Bremer et al bioRxiv [Preprint]. 2024 Nov 22:2024.11.

Insert

Gcn4 SATtoG KtoR

Tags

6xHis, TEV cleavage site

Expression

Bacterial

Mutation

S101G, T102G, S104G, T105G, S117G, K118R, T121G, …

Promoter

T7

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 9861 - 9880 of 10047 results

Results per page:

Sort results by: