We narrowed to 16,183 results for: nes
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Plasmid#170217PurposeBarcoded lentiviral vector to express Caspase8 (C360A) in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seqDepositorAvailable sinceSept. 23, 2021AvailabilityAcademic Institutions and Nonprofits only
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pSU312
Plasmid#169694PurposeTemplate plasmid for generating C-terminal 1xFLAG epitope tag fusions to bacterial genes by "Lambda red" recombineering.DepositorInsert1xFLAG epitope tag adjacent to FRT-flanked aph (KanR) gene
Tags1xFLAG tagExpressionBacterialAvailable sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTR(EF)-tRNA(Q)
Plasmid#170517PurposePCR template vector for amplifying gRNAcore(EF)-tRNA(Q) fragment; used together with pAC-U63-tgRNA-nlsBFP or pAC-U63-tgRNA-Gal80DepositorInsertgRNAcore(EF)-tRNA(Q)
UsePcr template vector for amplifying grnacore(ef)-t…Available sinceJune 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pW213-lenti-sasgRNA-Esp3I-2kb-filler-pEF1s-NLS-mNeonGreen-P2A-BlastR
Plasmid#170811PurposeLentiviral vector to co-express an sasgRNA with NLS-mNeonGreenDepositorTypeEmpty backboneUseLentiviralExpressionMammalianMutationNAAvailable sinceJune 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_mCherry_Rat_cebp-alfa
Plasmid#140098PurposeCRISPR-Cas9 library validation (positive control)DepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralPromotergRNA1 under U6 and gRNA2 under H1Available sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDECKO_mCherry_SPI1
Plasmid#140099PurposeCRISPR-Cas9 library validation (positive control)DepositorInsertgRNA1+scaffold+H1promoter+gRNA2
UseLentiviralPromotergRNA1 under U6 and gRNA2 under H1Available sinceApril 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-pax3a-kcnj13-IRES-EGFP
Plasmid#164950PurposeTol2 construct: pax3a-5.4k promoter driven zebrafish kcnj13 fused with EGFP and EGFPDepositorInsertkcnj13 (kcnj13 Zebrafish)
UseTol2 transposon destination vectorTagsIRES-EGFPPromoterpax3a promoter -5.4kAvailable sinceMarch 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDestTol2-actinb-kcnj13-IRES-EGFP
Plasmid#164941PurposeTol2 construct: actinb (actb2) promoter driven zebrafish kcnj13 and EGFPDepositorInsertkcnj13 (kcnj13 Zebrafish)
UseTol2 transposon destination vectorTagsIRES-EGFPPromoteractinb (actb2) promoterAvailable sinceFeb. 26, 2021AvailabilityAcademic Institutions and Nonprofits only -
BC1364-mouse U6-sgH2B
Plasmid#164043PurposeU6-driven sgRNA targeting H2BDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertH2B targeting sgRNA
UseCRISPRPromotermouse U6Available sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCY_gRNA_hPAX7-Pro_C6
Plasmid#160457PurposesgRNA targeting human PAX7 promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA_hPAX7-Promoter_C6
UseCRISPRExpressionMammalianPromoterU6Available sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCY_gRNA_hPAX7-Pro_C5
Plasmid#160456PurposesgRNA targeting human PAX7 promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA_hPAX7-Promoter_C5
UseCRISPRExpressionMammalianPromoterU6Available sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCY_gRNA_hPAX7-Pro_C4
Plasmid#160455PurposesgRNA targeting human PAX7 promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA_hPAX7-Promoter_C4
UseCRISPRExpressionMammalianPromoterU6Available sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCY_gRNA_hPAX7-Pro_C3
Plasmid#160454PurposesgRNA targeting human PAX7 promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA_hPAX7-Promoter_C3
UseCRISPRExpressionMammalianPromoterU6Available sinceNov. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-FLEXfrt-SaCas9-U6-sgGrin2a
Plasmid#124863PurposeMutagenesis of Grin2aDepositorInsertGrin2a (Grin2a Mouse)
UseAAV, CRISPR, and Mouse TargetingAvailable sinceMay 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pRAN357
Plasmid#120898PurposeE. coli-C. difficile shuttle vector: tetracycline-inducible promoter (Ptet) driving expression of cyan fluorescent protein (CfpOpt), multiple-cloning site for fusion to N-terminus of target proteinDepositorInsertcyan fluorescent protein, codon-optimized for low GC organisms, multiple-cloning site for protein fusion
ExpressionBacterialPromoterPtetAvailable sinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFA6a-hphMX6-3nmt1
Plasmid#105162PurposeYeast gene targetingDepositorInserthphMX6-3nmt1
Tags3nmt1ExpressionBacterial and YeastPromoter3nmt1Available sinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
RNAPII_CTD27-52_S1966C
Plasmid#98681PurposeExpresses 6xHis-tagged C-terminal domain of RNA polymerase II with S1966CDepositorInsertRNA polymerase II C-terminal domain (POLR2A Human)
Tags6xHisExpressionBacterialMutationS1966CAvailable sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
GFP-mROCK1-delta5
Plasmid#101293PurposeMammalian expression of Rock1-GFP for the study of localisation signaling.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRock1 (Rock1 Mouse)
ExpressionMammalianMutation375-727 aaPromoterCMVAvailable sinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-PC-2-CTS
Plasmid#86068PurposeA bacterial expression plasmid encoding polycystin-2 ciliary targeting signal with N-terminus GST-tag.DepositorInsertPolycystin-2 (Pkd2 Human)
TagsGSTExpressionBacterialMutationcontains the region from 1-15PromotertacAvailable sinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
tubulin-OeNL_pcDNA3
Plasmid#89523PurposeOrange color variant of bright luminescent protein, fused to b-tubulinDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertOeNL
Tagsb-tubulinExpressionMammalianPromoterCMVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only