We narrowed to 60,274 results for: cloning
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Plasmid#121012PurposeMoClo golden gate assembly CD part for Cas9 (S. pyogenes gRNA-targeted endonuclease Cas9). Please see Supplemental Documents for annotated Genbank file.DepositorInsertCas9
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
U2m
Plasmid#121015PurposeMoClo golden gate assembly BC part for BCD11 (Medium-high expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).DepositorInsertBCD11
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
U3m
Plasmid#121016PurposeMoClo golden gate assembly BC part for BCD13 (Medium expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).DepositorInsertBCD13
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
U5m
Plasmid#121018PurposeMoClo golden gate assembly BC part for BCD14 (Medium expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).DepositorInsertBCD14
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
U6m
Plasmid#121019PurposeMoClo golden gate assembly BC part for BCD20 (Medium expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).DepositorInsertBCD20
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
U7m
Plasmid#121020PurposeMoClo golden gate assembly BC part for BCD16 (low expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).DepositorInsertBCD16
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
U8m
Plasmid#121021PurposeMoClo golden gate assembly BC part for BCD24 (low expression bi-cistronic RBS, engineered for downstream context-independence; see https://doi.org/10.1038/nmeth.2404).DepositorInsertBCD24
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C84m
Plasmid#120995PurposeMoClo golden gate assembly CD part for sigma28/RpoF (RNA polymerase required for flagellar formation in E. coli). Please see Supplemental Documents for annotated Genbank file.DepositorInsertSigma 28
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C95m
Plasmid#121001PurposeMoClo golden gate assembly CD part for T7 RNA polymerase (use with P18m). Please see Supplemental Documents for annotated Genbank file.DepositorInsertT7 RNA polymerase (Andy please check!)
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C63m
Plasmid#120979PurposeMoClo golden gate assembly CD part for LacI (Lac repressor; identical to C1d but without ssrA degradation tag). Please see Supplemental Documents for annotated Genbank file.DepositorInsertlacI
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
C3m
Plasmid#120956PurposeMoClo golden gate assembly CD part for sfGFP (Superfolder green fluorescent protein from DOI: 10.1038/nbt1172; slight variant on https://www.fpbase.org/protein/superfolder-gfp/)DepositorInsertsfGFP
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
P30m
Plasmid#120942PurposeMoClo golden gate assembly AB part for pL-lacO1 (LacI-repressed promoter, as used in DOI:10.1038/35002125). Please see Supplemental Documents for annotated Genbank file.DepositorInsertpL-lacO1
UseSynthetic BiologyAvailable SinceDec. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAM-35s-Slac1-YFPn-1
Plasmid#102390Purposesplit YFP. Plant expression of Slac1-YFPn-1DepositorInsertSLAC1
TagsYFPnExpressionPlantPromoter35SAvailable SinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAM-35s-PBL27-YFPc-1
Plasmid#102388Purposesplit YFP. Plant expression of PBL27-YFPc-1DepositorInsertPBL27
TagsYFPcExpressionPlantPromoter35SAvailable SinceOct. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFG001
Plasmid#62816PurposeBacterial constitutive gRNA expression plasmidDepositorInsertgRNA
UseCRISPR and Synthetic BiologyExpressionBacterialPromoterPN25Available SinceNov. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBT1-proD-mCherry-DAS+4
Plasmid#65824PurposeConst. mCherry-DAS_4 expression , proD promoter, E. coli ExpressionDepositorInsertmCherry-DAS+4
ExpressionBacterialPromoterproD E . coli Constitutive PromoterAvailable SinceJune 24, 2015AvailabilityAcademic Institutions and Nonprofits only -
CDKL3A-c014
Plasmid#53684PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertCDKL3A
UseBaculovirus expressionTagsHis6-TEVPromoterpolyhedrin promoterAvailable SinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
CRK7A-c021
Plasmid#53643PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertCRK7A
UseBaculovirus expressionTagsHis6-TEVPromoterpolyhedrinAvailable SinceJuly 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
ERAP1A-c200
Plasmid#53675PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertERAP1A
UseBaculovirus expressionTagsHis6-TEVPromoterpolyhedrin promoterAvailable SinceJune 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
DCLRE1AA-c290
Plasmid#53682PurposeBaculovirus expression for structure determination; may not be full ORFDepositorInsertDCLRE1AA
UseBaculovirus expressionTagsHis6-TEVPromoterpolyhedrin promoterAvailable SinceJune 17, 2014AvailabilityAcademic Institutions and Nonprofits only