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MT2a_C21A

Plasmid

#105697

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 2a C21A mutant

Depositor

Artur Krężel

Publication

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

Metallothionein 2a (MT2A Human)

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Mutation

Cysteine 21 to Alanine

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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MT2a_K20A

Plasmid

#105698

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 2a K20A mutant

Depositor

Artur Krężel

Publication

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

Metallothionein 2a (MT2A Human)

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Mutation

Lysine 20 to Alanine

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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MT2a_S6A

Plasmid

#105699

Purpose

Bacterial expression plasmids for production of recombinant metallothionein 2a S6A mutant

Depositor

Artur Krężel

Publication

Singh et al Metallomics. 2023 Jun 1;15(6):mfad029. d

Insert

Metallothionein 2a (MT2A Human)

Tags

Chiting Binding Domanin-Intein

Expression

Bacterial

Mutation

Serine 6 to Alanine

Available since

April 27, 2020

Availability

Academic Institutions and Nonprofits only

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LMX1B-gRNA-C-del

Plasmid

#131516

Purpose

gRNA to delete a nucleation site near Lmx1b. Use with LMX1B-gRNA-N-del

Depositor

Danny Reinberg

Publication

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

LMX1B-gRNA-C-del

Use

CRISPR

Expression

Mammalian

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only
LMX1B-gRNA-N-del

Plasmid

#131333

Purpose

gRNA to delete a nucleation site near Lmx1b. Use with LMX1B-gRNA-C-del

Depositor

Danny Reinberg

Publication

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

LMX1B-gRNA-N-del

Use

CRISPR

Expression

Mammalian

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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HoxD del ds1

Plasmid

#131338

Purpose

gRNA to delete a nucleation site near HoxD. Use with HoxD del us1

Depositor

Danny Reinberg

Publication

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

EVX2-gRNA-C-del

Use

CRISPR

Expression

Mammalian

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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SKOR1-gRNA-C-del

Plasmid

#131332

Purpose

gRNA to delete a nucleation site near Skor1. Use with SKOR1-gRNA-N-del

Depositor

Danny Reinberg

Publication

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

SKOR1-gRNA-C-del

Use

CRISPR

Expression

Mammalian

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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SKOR1-gRNA-N-del

Plasmid

#131331

Purpose

gRNA to delete a nucleation site near Skor1. Use with SKOR1-gRNA-C-del

Depositor

Danny Reinberg

Publication

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

SKOR1-gRNA-N-del

Use

CRISPR

Expression

Mammalian

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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HoxD del control ds

Plasmid

#131336

Purpose

gRNA to delete control region near HoxD. Use with HoxD del control us

Depositor

Danny Reinberg

Publication

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

CTRL-gRNA-C-del

Use

CRISPR

Expression

Mammalian

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only
HoxD del us1

Plasmid

#131337

Purpose

gRNA to delete a nucleation site near HoxD. Use with HoxD del ds1

Depositor

Danny Reinberg

Publication

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

EVX2-gRNA-N-del

Use

CRISPR

Expression

Mammalian

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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HoxD del control us

Plasmid

#131335

Purpose

gRNA to delete control region near HoxD. Use with HoxD del control ds

Depositor

Danny Reinberg

Publication

Oksuz et al Mol Cell. 2018 Jun 21;70(6):1149-1162.e5

Insert

CTRL-gRNA-N-del

Use

CRISPR

Expression

Mammalian

Available since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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pMS22

Plasmid

#129710

Purpose

Expresses Escherichia coli RssB E134G:E135S:E136G:E137S variant as an N-terminal 10His fusion

Depositor

Alexandra Deaconescu

Publication

Dorich et al Genes Dev. 2019 Jun 1;33(11-12):718-732.

Insert

Escherichia coli RssB-GSGS variant (rssB Escherichia coli MG1655)

Tags

10-Histidine tag cleavable with PreScission Prote…

Expression

Bacterial

Mutation

RssB E134G:E135S:E136G:E137S

Promoter

T7

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/GUG-nLuc-3XFLAG-CL1/PEST

Plasmid

#127317

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from GUG start codon

Depositor

Jeremy Wilusz

Publication

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AUU-nLuc-3XFLAG

Plasmid

#127305

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AUU start codon

Depositor

Jeremy Wilusz

Publication

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AAA-nLuc-3XFLAG

Plasmid

#127306

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AAA start codon (Negative control plasmid)

Depositor

Jeremy Wilusz

Publication

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pGL4.13/AUG(30)-FFLuc-3XFLAG

Plasmid

#127336

Purpose

Express truncated 3xFLAG tagged firefly luciferase (FFLuc) from AUG at codon 30

Depositor

Jeremy Wilusz

Publication

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

Firefly luciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

SV40

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/CUG-nLuc-3XFLAG-PEST

Plasmid

#127313

Purpose

Express 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from CUG start codon

Depositor

Jeremy Wilusz

Publication

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and PEST

Expression

Mammalian

Promoter

CMV

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AUU-nLuc-3XFLAG-CL1/PEST

Plasmid

#127319

Purpose

Express 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AUU start codon

Depositor

Jeremy Wilusz

Publication

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG and CL1/PEST

Expression

Mammalian

Promoter

CMV

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/AGG-nLuc-3XFLAG

Plasmid

#127304

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AGG start codon

Depositor

Jeremy Wilusz

Publication

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/ACG-nLuc-3XFLAG

Plasmid

#127303

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from ACG start codon

Depositor

Jeremy Wilusz

Publication

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available since

June 25, 2019

Availability

Academic Institutions and Nonprofits only

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