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pLAP-BUBR1-3A

Plasmid

#114054

Purpose

transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

Depositor

Geert Kops

Article

Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

Insert

BUBR1-3A (BUB1B Human)

Tags

YFP-TEV-S- Human LAP

Expression

Mammalian

Mutation

C2823A and G2826A (siRNA-resistance), BUBR1-S670A…

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pLAP-BUBR1-3D

Plasmid

#114055

Purpose

transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

Depositor

Geert Kops

Article

Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

Insert

BUBR1-3D (BUB1B Human)

Tags

YFP-TEV-S- Human LAP

Expression

Mammalian

Mutation

C2823A and G2826A (siRNA-resistance), BUBR1-S670D…

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pMCh-1539

Plasmid

#82432

Purpose

Plasmid for expression of GST-tagged human-Nematostella GW182 chimera in mammalian cells

Depositor

Marina Chekulaeva

Article

Mauri et al Nucleic Acids Res. 2016 Sep 6. pii: gkw7

Insert

hs-nvGW182 chimera

Tags

GST

Expression

Mammalian

Mutation

aa 1-1169 of siRNA resistant hsTNRC6A (Q8NDV7-2) …

Promoter

EF1A

Available Since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pFM034

Plasmid

#40137

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant (wild-type) (par-2 )

Use

Gateway Cloning

Tags

GFP

Expression

Worm

Mutation

Base pairs 6-523 of par-2 were recoded to create…

Promoter

pie-1

Available Since

Sept. 6, 2012

Availability

Academic Institutions and Nonprofits only
UbC Puro ABL FRET F72P

Plasmid

#235033

Purpose

Expression of BCR-ABL F72P FRET biosensor

Depositor

Justin Pritchard

Article

Sokirniy et al Genome Med. 2026 Jun 27. doi: 10.1186/s1

Insert

BCR-ABL with internal StayGold and mScarlet3 tags (ABL1 Human)

Use

Lentiviral

Tags

PuroR-P2A

Mutation

F72P

Promoter

human ubiquitin C

Available Since

April 30, 2026

Availability

Academic Institutions and Nonprofits only

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UbC Puro ABL FRET P112L

Plasmid

#235034

Purpose

Expression of BCR-ABL P112L FRET biosensor

Depositor

Justin Pritchard

Article

Sokirniy et al Genome Med. 2026 Jun 27. doi: 10.1186/s1

Insert

BCR-ABL with internal StayGold and mScarlet3 tags (ABL1 Human)

Use

Lentiviral

Tags

PuroR-P2A

Mutation

P112L

Promoter

human ubiquitin C

Available Since

April 30, 2026

Availability

Academic Institutions and Nonprofits only

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UbC Puro ABL FRET V73A

Plasmid

#235036

Purpose

Expression of BCR-ABL V73A FRET biosensor

Depositor

Justin Pritchard

Article

Sokirniy et al Genome Med. 2026 Jun 27. doi: 10.1186/s1

Insert

BCR-ABL with internal StayGold and mScarlet3 tags (ABL1 Human)

Use

Lentiviral

Tags

PuroR-P2A

Mutation

V73A

Promoter

human ubiquitin C

Available Since

April 30, 2026

Availability

Academic Institutions and Nonprofits only

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UbC Puro ABL FRET Y253H

Plasmid

#235037

Purpose

Expression of BCR-ABL Y253H FRET biosensor

Depositor

Justin Pritchard

Article

Sokirniy et al Genome Med. 2026 Jun 27. doi: 10.1186/s1

Insert

BCR-ABL with internal StayGold and mScarlet3 tags (ABL1 Human)

Use

Lentiviral

Tags

PuroR-P2A

Mutation

Y253H

Promoter

human ubiquitin C

Available Since

April 30, 2026

Availability

Academic Institutions and Nonprofits only

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UbC Puro ABL FRET M244V

Plasmid

#235039

Purpose

Expression of BCR-ABL M244V FRET biosensor

Depositor

Justin Pritchard

Article

Sokirniy et al Genome Med. 2026 Jun 27. doi: 10.1186/s1

Insert

BCR-ABL with internal StayGold and mScarlet3 tags (ABL1 Human)

Use

Lentiviral

Tags

PuroR-P2A

Mutation

M244V

Promoter

human ubiquitin C

Available Since

April 30, 2026

Availability

Academic Institutions and Nonprofits only

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UbC Puro ABL FRET Y342A

Plasmid

#235040

Purpose

Expression of BCR-ABL Y342A FRET biosensor

Depositor

Justin Pritchard

Article

Sokirniy et al Genome Med. 2026 Jun 27. doi: 10.1186/s1

Insert

BCR-ABL with internal StayGold and mScarlet3 tags (ABL1 Human)

Use

Lentiviral

Tags

PuroR-P2A

Mutation

Y342A

Promoter

human ubiquitin C

Available Since

April 30, 2026

Availability

Academic Institutions and Nonprofits only

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hsp70I: cPla-mKate2-P2A-EGFP-KDEL

Plasmid

#250915

Purpose

Dual color expression of mKate2 tagged zebrafish cPla2 and ER markers (EGFP-KDEL) in zebrafish live tissue after heat shock

Depositor

Philipp Niethammer

Article

Shen et al Nat Cell Biol. 2025 Dec 9. doi: 10.1038/

Insert

cPla2-mKate2-P2A-EGFP-KDEL (pla2g4aa Zebrafish)

Use

D.rerio (zebrafish)

Tags

KDEL on EGFP and mKate2 on cPla2

Promoter

hsp70i

Available Since

Jan. 29, 2026

Availability

Academic Institutions and Nonprofits only

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CD44v-EC-His (pcDNA3)

Plasmid

#238418

Purpose

Expresses the polyhistidine-tagged extracellular region of CD44v (CD44v-EC-His)

Depositor

Amy Shiu Lee

Article

Tseng et al Life Sci Alliance. 2019 Aug 15;2(4):e201

Insert

CD44 containing extracellular region of CD44v (CD44 Human)

Tags

His

Expression

Mammalian

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pUN1213 - pL0_p16OMT (pro + 5U)

Plasmid

#203890

Purpose

16OMT promoter and native 5'UTR (approximately 1 kb upstream of start codon) from C. roseus in a MoClo compatible L0 plasmid.

Depositor

Carolyn Lee-Parsons

Article

Cole-Osborn et al Plant Cell Rep. 2024 May 14;43(6):141. d

Insert

16OMT promoter and 5'UTR

Expression

Plant

Mutation

Mutation of BpiI and/or BsaI cut sites for MoClo …

Available Since

April 26, 2024

Availability

Academic Institutions and Nonprofits only

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pLX304-BCL-XL-499/500R

Plasmid

#203575

Purpose

Expresses V5-tagged BCL-XL with resistance to shRNA (TRCN0000033499, TRCN0000033500) in mammalian cells.

Depositor

Abhishek Chakraborty

Article

Grubb et al Clin Cancer Res. 2022 Nov 1;28(21):4689-

Insert

BCL2L1 (BCL2L1 Human)

Use

Lentiviral

Tags

V5-tagged

Expression

Mammalian

Mutation

silent point mutations to make resistant shRNA 49…

Promoter

CMV

Available Since

Feb. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-V1-rCD20-BSD

Plasmid

#209752

Purpose

To reconstitute CD20 expression with a specific mRNA isoform (variant 1) after CRISPR-Cas9-mediated knockout of CD20.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Mutation

The CCTGGGGGGTCTTCTGATGATCC sequence within the C…

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-V2-rCD20-BSD

Plasmid

#209753

Purpose

To reconstitute CD20 expression with a specific mRNA isoform (variant 2) after CRISPR-Cas9-mediated knockout of CD20.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Mutation

The CCTGGGGGGTCTTCTGATGATCC sequence within the C…

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCDH-V3-rCD20-BSD

Plasmid

#209754

Purpose

To reconstitute CD20 expression with a specific mRNA isoform (variant 3) after CRISPR-Cas9-mediated knockout of CD20.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Mutation

The CCTGGGGGGTCTTCTGATGATCC sequence within the C…

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pRK5 PSK (745-1235)

Plasmid

#197863

Purpose

Expression of C-terminal PSK1 / TAOK2 (aa745-1235) in mammalian cells (microtubule-binding domain)

Depositor

Jonathan Morris

Article

Mitsopoulos et al J Biol Chem. 2003 May 16;278(20):18085-9

Insert

PSK1 (745-1235) (TAOK2 Human)

Tags

Myc

Expression

Mammalian

Mutation

The N terminus of PSK1/TAOK2 has been deleted

Promoter

SV40

Available Since

March 24, 2023

Availability

Academic Institutions and Nonprofits only

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kanMX6-ins1

Plasmid

#195038

Purpose

pFA6a derived selection cassette flanked with transcription terminators (tDEG1/tDEG1), allows genome modification without disruption of insertion neighboring genes by transcription interference

Depositor

Gloria Brar

Article

Powers et al Elife. 2022 Dec 12;11:e81086. doi: 10.75

Insert

KanR

Use

Yeast genomic targeting

Tags

S. cerevisiae DEG1 terminator, A. gossypii TEF pr…

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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KCNJ15_pcDNA6.2/EmGFP-Bsd

Plasmid

#176981

Purpose

Mammalian expression vector encoding KCNJ15 and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

KCNJ15 (KCNJ15 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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