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GFP-Rnd1

Plasmid

#23227

Depositor

Channing Der

Article

Roberts et al J Biol Chem. 2008 Sep 12. 283(37):25150-

Insert

Rnd1 (RND1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

March 24, 2010

Availability

Academic Institutions and Nonprofits only
pEN_TmiRc3

Plasmid

#25748

Purpose

Entry vector for cloning miR30-based shRNA driven by TRE promoter.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Type

Empty backbone

Use

Entry vector

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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Epsin1 deltaUIM-ECFP

Plasmid

#20973

Depositor

Xiaowei Zhuang

Article

Chen et al Proc Natl Acad Sci U S A. 2008 Aug 19. 1

Insert

Epsin1 deltaUIM (Epn1 Rat)

Tags

ECFP

Expression

Mammalian

Available Since

June 15, 2009

Availability

Academic Institutions and Nonprofits only
pBzCas13b-gRNA-NT

Plasmid

#164861

Purpose

Constitutive expression of single-spacer CRISPR array with non-targeting spacer for BzCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

BzCas13b-gRNA-NT

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pBzCas13b-gRNA-1

Plasmid

#164858

Purpose

Constitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for BzCas13b in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Methods Mol Biol. 2022;2404:135-153. doi

Insert

BzCas13b-gRNA-1

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 22, 2021

Availability

Academic Institutions and Nonprofits only

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PGEX-NEDD4

Plasmid

#45043

Depositor

Shiaw-Yih Lin

Article

Yim et al Cancer Cell. 2009 Apr 7;15(4):304-14. do

Insert

NEDD4-1 (NEDD4 Human)

Tags

GST

Expression

Bacterial

Available Since

June 6, 2013

Availability

Academic Institutions and Nonprofits only
pGateway 3XFlag Prickle2 flag tagged

Plasmid

#24645

Depositor

Jeff Wrana

Article

Narimatsu et al Cell. 2009 Apr 17. 137(2):295-307.

Insert

Prickle 2 (PRICKLE2 Human)

Tags

3XFLAG

Expression

Mammalian

Available Since

April 30, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pET28a-SnoopTagJr-MBP

Plasmid

#105628

Purpose

Plasmid for recombinant expression in bacteria of MBP fused to SnoopTagJr, permitting covalent linkage to DogTag by SnoopLigase

Depositor

Mark Howarth

Article

Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

Insert

SnoopTagJr-MBP

Expression

Bacterial

Promoter

T7

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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Bxb1_tdTomato

Plasmid

#198041

Purpose

Bxb1-specific donor plasmid for integrating a transcription unit for constitutive expression of tdTomato reporter.

Depositor

Richard Davis

Article

Blanch-Asensio et al Nat Protoc. 2024 Aug 23. doi: 10.1038/s4

Insert

tdTomato

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available Since

May 16, 2023

Availability

Academic Institutions and Nonprofits only

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pQP-CiRISB

Plasmid

#138978

Purpose

Light-controlled tri-directional targeting of endogenous proteins (cytoplasm-shifted)

Depositor

Vladislav Verkhusha

Article

Redchuk et al Nat Commun. 2020 Jan 30;11(1):605. doi:

Insert

NcoI-BphP1-CAAX-IRES2-iB(GFP)-NESmCherry- Q-PAS1-AsLOV2cNLS-NotI

Available Since

April 23, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro U6 sgRNA CAG

Plasmid

#50927

Purpose

U6 driven sgRNA negative control

Depositor

Rene Maehr, Scot Wolfe

Article

Kearns et al Development. 2014 Jan;141(1):219-23. doi

Insert

negative control sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/Puro/TagRFP-LL5b

Plasmid

#112828

Purpose

transient/stable overexpression

Depositor

Tomasz Prószyński, Joshua Sanes

Article

Proszynski et al J Cell Sci. 2013 May 15;126(Pt 10):2225-

Insert

LL5b (Phldb2 Mouse)

Tags

TagRFP

Expression

Mammalian

Available Since

Nov. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-Lifeact EGFP

Plasmid

#187686

Purpose

In vivo visualization of actin

Depositor

Mary Beckerle

Article

Smith et al PLoS One. 2013 Aug 21;8(8):e69378. doi:

Insert

Lifeact

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pEF-APEX2-NLS

Plasmid

#140281

Purpose

Expression of APEX2-NLS

Depositor

Yusuke Miyanari

Article

Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

Insert

APEX2-NLS

Expression

Mammalian

Promoter

EF1a

Available Since

May 4, 2020

Availability

Academic Institutions and Nonprofits only

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RAB14 shRNA

Plasmid

#61496

Purpose

to knockdown endogenous RAB14 expression levels

Depositor

Curt Civin

Article

Kim et al Br J Haematol. 2014 Oct 14. doi: 10.1111

Insert

RAB14 (RAB14 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

hU6

Available Since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pET28a-SUMO-DogTag

Plasmid

#105629

Purpose

Plasmid for recombinant expression in bacteria of SUMO domain fused to DogTag, permitting covalent linkage to SnoopTagJr

Depositor

Mark Howarth

Article

Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3

Insert

SUMO-DogTag

Expression

Bacterial

Promoter

T7

Available Since

March 16, 2018

Availability

Academic Institutions and Nonprofits only

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GAK-pmCherryN1

Plasmid

#27695

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

GAK (Gak Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only
Ca-FLARE (protease)

Plasmid

#92214

Purpose

protease component of FLARE

Depositor

Alice Ting

Article

Wang et al Nat Biotechnol. 2017 Jun 26. doi: 10.103

Insert

CaM-V5-TEVp(Δ220-242)

Use

AAV

Expression

Mammalian

Promoter

synapsin

Available Since

June 16, 2017

Availability

Academic Institutions and Nonprofits only

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pETZZtevPP7CP(C68A,C73A)

Plasmid

#27548

Depositor

Kathleen Collins

Article

Hogg et al RNA. 2007 Jun . 13(6):868-80.

Insert

Pseudomonas phage PP7 coat protein (cp Pseudomonas phage PP7)

Tags

TEV protease cleavage site, six histidine tag, an…

Expression

Bacterial

Mutation

changed two cysteine to alanine (C68A,C73A) to in…

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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human pirh2-(1-261)

Plasmid

#24878

Depositor

Cheryl Arrowsmith

Article

Sheng et al Nat Struct Mol Biol. 2008 Dec . 15(12):1

Insert

Pirh2 (RCHY1 Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 5, 2010

Availability

Academic Institutions and Nonprofits only