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pJ211_WT_med

Plasmid

#128849

Purpose

Medium copy number plasmid for expression of E. coli flagellin from its natural promoter

Depositor

Mark Pallen

Article

Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

Insert

Flagellin

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pJ211_rha_WT_hi

Plasmid

#128853

Purpose

High copy number plasmid for expression of E. coli flagellin from a rhamnose-inducible promoter

Depositor

Mark Pallen

Article

Thomson et al Curr Res Biotechnol. 2020 Nov;2:45-52. d

Insert

Flagellin

Expression

Bacterial

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pUC19_TTE1564_UUCG-loop

Plasmid

#133581

Purpose

T7-transcription template for a P2-deletion mutation of the Tte preQ1- riboswitch

Depositor

Nils Walter

Article

Lund et al Nucleic Acids Res. 2019 Dec 13. pii: 567

Insert

P2-deletion mutant of Tte preQ1-riboswitch

Use

Unspecified

Mutation

replacement of riboswitch nucleotides U6 through …

Promoter

T7

Available Since

Jan. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pMM765

Plasmid

#133602

Purpose

Part Plasmid for dCas9 NLS Stop, Part 3(coding sequence)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

dCas9 NLS Stop

Use

Synthetic Biology

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pMM756

Plasmid

#133600

Purpose

Part Plasmid for ERG25 sgRNA, Part 234(promoter,coding region, terminator)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

sgERG25

Use

Synthetic Biology

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pMM729

Plasmid

#133598

Purpose

Part Plasmid for Mxi1-dCas9, Part 3(coding sequence)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

Mxi1-dCas9

Use

Synthetic Biology

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pMM767

Plasmid

#133604

Purpose

Part Plasmid for dCas9 NLS, Part 3(coding sequence)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

dCas9 NLS

Use

Synthetic Biology

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pMM585

Plasmid

#133591

Purpose

Part Plasmid for dCas9, Part 3a(coding sequence)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

dCas9

Use

Synthetic Biology

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pMM766

Plasmid

#133603

Purpose

Part Plasmid for dCas9 NLS Stop, Part 3b(coding sequence)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

dCas9 NLS Stop

Use

Synthetic Biology

Available Since

Dec. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pMM768

Plasmid

#133605

Purpose

Part Plasmid for dCas9 NLS, Part 3b(coding sequence)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

dCas9 NLS

Use

Synthetic Biology

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pMM769

Plasmid

#133606

Purpose

Part Plasmid for dCas9 NLS, Part 3a(coding sequence)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

dCas9 NLS

Use

Synthetic Biology

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pMM755

Plasmid

#133599

Purpose

Part Plasmid for ERG11 sgRNA, Part 234(promoter,coding region, terminator)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

sgERG11

Use

Synthetic Biology

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pMM764

Plasmid

#133601

Purpose

Part Plasmid for TEF1 sgRNA, Part 234(promoter,coding region, terminator)

Depositor

Megan McClean

Article

Geller et al Cell Mol Bioeng. 2019 Sep 24;12(5):511-5

Insert

sgTEF1

Use

Synthetic Biology

Available Since

Dec. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pSADuet148

Plasmid

#121910

Purpose

Charge-introduced NpuDnaBΔ283ΔC39 intein

Depositor

Hideo Iwai

Article

Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

Insert

CI-NpuDnaBΔ290ΔC39 intein

Tags

His6-GB1

Expression

Bacterial

Mutation

Deletion of 290-residue endonuclease domain, I53K…

Promoter

T7

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pHBDuet259

Plasmid

#121911

Purpose

NpuDnaBΔ283ΔC39 intein

Depositor

Hideo Iwai

Article

Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

Insert

NpuDnaBΔ283ΔC39 intein

Tags

His6-GB1

Expression

Bacterial

Mutation

Deletion of 283-residue endonuclease domain

Promoter

T7

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pHBBAD113

Plasmid

#121912

Purpose

Charge-Introduced NpuDnaBC39 intein

Depositor

Hideo Iwai

Article

Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

Insert

CI-NpuDnaBC39 intein

Tags

GB1-His6

Expression

Bacterial

Mutation

S110K, I111K

Promoter

Para

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pHBDuet116

Plasmid

#121915

Purpose

NpuDnaBΔ290ΔC39 intein

Depositor

Hideo Iwai

Article

Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

Insert

Oth-NpuDnaBΔ290ΔC39 intein

Tags

His6-GB1

Expression

Bacterial

Mutation

Deletion of 290-residue endonuclease domain, I53K…

Promoter

T7

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pHBBAD168

Plasmid

#121916

Purpose

Oth-NpuDnaBC39 intein

Depositor

Hideo Iwai

Article

Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

Insert

Oth-NpuDnaBC39 intein

Tags

GB1-His6

Expression

Bacterial

Mutation

I111K, E116K

Promoter

Para

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pZF-BBB

Plasmid

#127470

Purpose

coding sequence for synthetic zinc finger ZF-BBB

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-BBB synthetic transcription factor

Use

Cell-free transcription–translation

Promoter

T7

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pZF-BCC

Plasmid

#127484

Purpose

coding sequence for synthetic zinc finger ZF-BCC

Depositor

Sebastian Maerkl

Article

Swank et al Proc Natl Acad Sci U S A. 2019 Mar 26;11

Insert

ZF-BCC synthetic transcription factor

Use

Cell-free transcription–translation

Promoter

T7

Available Since

Nov. 15, 2019

Availability

Academic Institutions and Nonprofits only

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