We narrowed to 14,166 results for: flag

1. pDB-hCRY1-mScarlet-I-CD4-bla

   Plasmid

   #179442

   Purpose

   Donor vector to knock in mScarlet-I C-terminal to human CRY1 gene

   Depositor

   Achim Kramer

   Article

   Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

   Insert

   mScarletI
   

   Use

   CRISPR

   Tags

   His/Flag

   Promoter

   None

   Available Since

   March 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCR4_miniFXN_8

   Plasmid

   #234361

   Purpose

   Mini FXN plasmid with a partial deletion of the 5' UTR and a large deletion of intron 1 in FXN. Does not contain GAA repeats.

   Depositor

   Marek Napierala

   Article

   Li et al Hum Gene Ther. 2020 Aug;31(15-16):839-85

   Insert

   FXN (FXN Human)
   

   Use

   Cloning

   Tags

   Flag Tag

   Mutation

   There is a partial deletion in the 5'UTR of …

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. dCas9-GCN5 FL

   Plasmid

   #179552

   Purpose

   encodes S. pyogenes dCas9 with c-terminal fusion of full length human GCN5 driven by EF-1alpha promoter

   Depositor

   Isaac Hilton

   Article

   Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

   Insert

   S. pyogenes dCas9 with c-terminal fusion of full length human GCN5 (KAT2A Human)
   

   Use

   Lentiviral

   Tags

   Flag Tag

   Expression

   Mammalian

   Mutation

   D10A; H840A in S.pyogenes Cas9

   Promoter

   EF-1alpha

   Available Since

   Feb. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAT100

   Plasmid

   #180512

   Purpose

   Plasmid expressing mammalian codon optimized engineered chimeric PlmCasX-R1, sgRNAv2 scaffold, and restriction sites to clone in new spacers

   Depositor

   Jennifer Doudna

   Article

   Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

   Inserts

   CasX sgRNAv2
   PlmCasX with DpbCasX R1 loop
   

   Use

   CRISPR

   Tags

   2x FLAG and SV40 NLS

   Expression

   Mammalian

   Promoter

   CAG and U6

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV_hsyn_NES-his-CaMPARI2-F391W-WPRE-SV40

   Plasmid

   #101061

   Purpose

   AAV vector expressing CaMPARI2_F391W (Kd = 110nM), a photoconvertible fluorescent protein-based calcium integrator, in neurons

   Depositor

   Eric Schreiter

   Article

   Moeyaert et al Nat Commun. 2018 Oct 25;9(1):4440. doi:

   Has Service

   AAV1

   Insert

   CaMPARI2-F391W
   

   Use

   AAV

   Tags

   FLAG-HA-myc and NES_his

   Promoter

   hsyn (synapsin-1)

   Available Since

   Oct. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. Parvalbumin scFv [L114/3]

   Plasmid

   #190504

   Purpose

   Mammalian Expression of Parvalbumin scFV. Derived from hybridoma L114/3.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Parvalbumin (Rattus norvegicus) recombinant scFV (Pvalb Mouse)
   

   Tags

   HA, 6xHis, Flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRetroX SgK269 WT

   Plasmid

   #68028

   Purpose

   expression of Sgk269 in mammalian cells

   Depositor

   Roger Daly

   Article

   Croucher et al Cancer Res. 2013 Mar 15;73(6):1969-80. d

   Insert

   PEAK1 (PEAK1 Human)
   

   Use

   Retroviral

   Tags

   FLAG/myc, HA, and IRES-DsRed

   Expression

   Mammalian

   Available Since

   Nov. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pTRE-TAZ-CAMTA1

   Plasmid

   #235680

   Purpose

   Express TAZ-CAMTA1 fusion gene in mammalian cells using the doxycycline-inducible TRE promoter

   Depositor

   Duojia Pan

   Article

   Driskill et al Genes Dev. 2021 Apr 1;35(7-8):495-511. d

   Insert

   TAZ-CAMTA1 (WWTR1, CAMTA1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCMV-Tag2B EDD C2768A

   Plasmid

   #37189

   Depositor

   Darren Saunders, Charles Watts

   Article

   Henderson et al J Biol Chem. 2002 Jul 19;277(29):26468-7

   Insert

   EDD C2768A (UBR5 Human)
   

   Tags

   6x His Tag and FLAG

   Expression

   Mammalian

   Mutation

   Changed Cysteine 2768 to Alanine

   Promoter

   CMV

   Available Since

   July 26, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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10. Lenti-EFS-FLTID-GSQ-PMLivaWT-P2A-BLAST

    Plasmid

    #208040

    Purpose

    Enables constitutive expression of N-terminal TurboID-fused PML (isoform IVa; WT); selection with blasticidin

    Depositor

    Rosa Barrio, James Sutherland

    Article

    Barroso-Gomila et al Nat Commun. 2021 Nov 18;12(1):6671. doi:

    Insert

    PML (isoform IVa) (PML Human)
    

    Use

    Lentiviral

    Tags

    FLAG, TurboID

    Promoter

    EFS

    Available Since

    Oct. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAT527

    Plasmid

    #180514

    Purpose

    Plasmid expressing mammalian codon optimized engineered chimeric PlmCasX-R1, mNeonGreen, sgRNAv2 scaffold, and restriction sites to clone in new spacers

    Depositor

    Jennifer Doudna

    Article

    Tsuchida et al Mol Cell. 2022 Mar 17;82(6):1199-1209.e6

    Inserts

    CasX sgRNAv2
    PlmCasX with DpbCasX R1 loop-2A-mNeonGreen
    

    Use

    CRISPR

    Tags

    2x FLAG and SV40 NLS

    Expression

    Mammalian

    Promoter

    CAG and U6

    Available Since

    April 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. U6-hGRIN2B-CAG-ps-SpCas9

    Plasmid

    #102851

    Purpose

    A single-chain light-controllable dSpCas9 with pdDronpa1 domains for hGRIN2B gene editing

    Depositor

    Michael Lin

    Article

    Zhou et al ACS Chem Biol. 2017 Sep 29. doi: 10.1021

    Insert

    sp-hGRIN2B-sgRNA; dSpCas9; pdDronpa1 (GRIN2B Human, Synthetic, S. pyogenes)
    

    Use

    CRISPR

    Tags

    3X Flag and NLS

    Expression

    Mammalian

    Promoter

    U6 promoter

    Available Since

    Nov. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. lenti U6-sgRNA-acRNA SYN-dCas9-P2A-EGFP

    Plasmid

    #159086

    Purpose

    Expresses dCas9-P2A-EGFP driven by human SYN promoter and empty CRISPR Display sgRNA/accessory RNA from U6 promoter.

    Depositor

    Jeremy Day

    Article

    Carullo et al Nucleic Acids Res. 2020 Aug 18. pii: 589

    Insert

    empty crRNA-acRNA backbone, dCas9-P2A-EGFP
    

    Use

    CRISPR and Lentiviral

    Tags

    EGFP and FLAG

    Expression

    Mammalian

    Promoter

    hSYN, U6

    Available Since

    Sept. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3 MLL2 653

    Plasmid

    #11017

    Depositor

    Matthew Meyerson

    Article

    Hughes et al Mol Cell. 2004 Feb 27. 13(4):587-97.

    Insert

    MLL2 (KMT2B Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    truncation of MLL2, last 653 amino acids, from pr…

    Available Since

    Jan. 5, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pLenti-HF1RA-P2A-GFP-PGK-Puro

    Plasmid

    #110866

    Purpose

    Lentiviral vector for constitutive expression of Cas9-HF1RA-P2A-GFP in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    Cas9-HF1RA
    

    Use

    Lentiviral

    Tags

    FLAG

    Mutation

    R661A, Q695A, Q926A, and NLS sequence at the N-te…

    Promoter

    EF1s

    Available Since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pYPQ166-SpRY

    Plasmid

    #161520

    Purpose

    Gateway entry plasmid (attL1 & attR5) expressing 3_FLAG-NLS-zSpRYCas9-NLS without promoter

    Depositor

    Yiping Qi

    Article

    Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

    Insert

    zSpRYCas9
    

    Use

    CRISPR; Gateway compatible zsprycas9 entry clone

    Tags

    3X FLAG, NLS and NLS

    Expression

    Plant

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. lentiCas9-Venus

    Plasmid

    #70267

    Purpose

    Expresses human codon-optimized S. pyogenes Cas9 protein and Venus reporter from EFS promoter. Lentiviral backbone.

    Depositor

    Daniel Bauer

    Article

    Canver et al Nature. 2015 Sep 16. doi: 10.1038/nature

    Inserts

    Cas9
    Venus Reporter
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    EFS-NS and P2A

    Available Since

    Feb. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. FUW-tetO-YAPS94A

    Plasmid

    #84010

    Purpose

    expresses a TEAD-binding mutant of YAP (YAPS94A) in mammalian cells under the control of a doxycycline-inducible promoter

    Depositor

    Stefano Piccolo

    Article

    Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5

    Insert

    YAP1 (siRNA insensitive) (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S94A

    Promoter

    TRE-CMV

    Available Since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. pDB-hPER2-Luciferase-hvTK-bla

    Plasmid

    #179450

    Purpose

    Donor vector to knock in firefly Luciferase C-terminal to human PER2 gene

    Depositor

    Achim Kramer

    Article

    Gabriel et al Nat Commun. 2021 Jun 18;12(1):3796. doi:

    Insert

    Luciferase
    

    Use

    CRISPR

    Tags

    His/Flag

    Promoter

    None

    Available Since

    March 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLenti-xFNLS-P2A-Puro

    Plasmid

    #110872

    Purpose

    Lentiviral vector for constitutive expression of xFNLS in mammalian cells (codon optimized)

    Depositor

    Lukas Dow

    Article

    Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

    Insert

    xFNLS(3.7)
    

    Use

    Lentiviral

    Tags

    3X FLAG

    Mutation

    D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

    Promoter

    EF1s

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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