We narrowed to 1,234 results for: cfp

1. mCit-4aa-SNAP25B

   Plasmid

   #53235

   Purpose

   Fluorescent probe: SNAP-25B, N-terminally-tagged with monomeric Citrine, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCer-4aa-VAMP2 to measure FRET and dispersion.

   Depositor

   Robert Zucker

   Article

   Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

   Insert

   SNAP-25B (Snap25 Rat)
   

   Tags

   4aa-linker and mCitrine

   Expression

   Mammalian

   Available Since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGWB445

   Plasmid

   #74831

   Purpose

   Gateway cloning compatible binary vector for N-terminal fusion with eCFP (CaMV35S promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Promoter

   CaMV35S

   Available Since

   Nov. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSLQ10333

   Plasmid

   #183955

   Purpose

   CAG-dCas12a(WT)-HA-miniVPR-mCherry-T2A-HygroR

   Depositor

   Stanley Qi

   Article

   Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

   Insert

   Nuclease-dead Lb Cas12a
   

   Use

   CRISPR

   Tags

   HA, Hygromycin Resistance, and mCherry

   Expression

   Mammalian

   Mutation

   D832A

   Promoter

   CAG

   Available Since

   May 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. mCer-4aa-VAMP2

   Plasmid

   #53233

   Purpose

   Fluorescent probe: VAMP2 (synaptobrevin2), N-terminally-tagged with monomeric Cerulean, connected with a 4 amino acid linker. Expressed in mammalian cells, used with mCit-4aa-SNAP25B for FRET.

   Depositor

   Robert Zucker

   Article

   Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

   Insert

   VAMP2 (Vamp2 Rat)
   

   Tags

   4aa-linker and mCerulean

   Expression

   Mammalian

   Available Since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

5. 15x-QUAS-dTomato-T2A-TRPV1

   Plasmid

   #140945

   Purpose

   QUAS reporter line that expresses cytosolic dTomato and the rat TRPV1 ion channel, separated by T2A

   Depositor

   Leslie Vosshall

   Article

   Jove et al Neuron. 2020 Dec 23;108(6):1163-1180.e12

   Insert

   15x-QUAS-dTomato-T2A-TRPV1
   

   Available Since

   Oct. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSCV PIG Pu.1

   Plasmid

   #66982

   Purpose

   Retroviral expression plasmid for mouse Pu.1 expression; containing bicistronic IRES-GFP

   Depositor

   Christopher Vakoc

   Article

   Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

   Insert

   Spi1 (Spi1 Mouse)
   

   Use

   Retroviral

   Promoter

   LTR

   Available Since

   July 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGWB543

   Plasmid

   #74877

   Purpose

   Gateway cloning compatible binary vector for C-terminal fusion with eCFP (no promoter).

   Depositor

   Tsuyoshi Nakagawa

   Article

   Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

   Type

   Empty backbone

   Expression

   Plant

   Available Since

   Oct. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. Twitch-4 pcDNA3

   Plasmid

   #49533

   Purpose

   Fluorescent reporter for calcium signaling

   Depositor

   Oliver Griesbeck

   Article

   Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

   Insert

   Twitch-4
   

   Tags

   ECFP and cpCit174

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 14, 2014

   Availability

   Academic Institutions and Nonprofits only

9. pSL1180-HR-PUbEBFP2-NLS

   Plasmid

   #176682

   Purpose

   Expression of nuclear localized EBFP2 under Ae. aegypti Polyubiquitin promoter (AAEL003888), EBFP2 version of pSL1180-HR-PUbECFP (Addgene #47917)

   Depositor

   Norbert Perrimon

   Article

   Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

   Insert

   EBFP2
   

   Expression

   Insect

   Promoter

   Ae. aegypti poly-ubiquitin (AAEL003888)

   Available Since

   Nov. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. Twitch-1 pcDNA3

    Plasmid

    #49812

    Purpose

    Fluorescent reporter for calcium signaling

    Depositor

    Oliver Griesbeck

    Article

    Thestrup et al Nat Methods. 2014 Jan 5. doi: 10.1038/nm

    Insert

    Twitch-1
    

    Tags

    ECFP and cpCit174

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 16, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pGWB545

    Plasmid

    #74879

    Purpose

    Gateway cloning compatible binary vector for N-terminal fusion with eCFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Promoter

    CaMV35S

    Available Since

    Oct. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. Fyn-Fyn Biosensor

    Plasmid

    #124655

    Purpose

    FRET biosensor. Fyn-tagged biosensor targeting to membrane

    Depositor

    Yingxiao Wang

    Article

    Ouyang et al ACS Sens. 2019 Jan 25;4(1):76-86. doi: 1

    Insert

    Synthetic gene containing ECFP-SH2 domain-substrate-linker-YPet
    

    Expression

    Mammalian

    Available Since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. VAMP2-12aa-mCit

    Plasmid

    #53237

    Purpose

    Fluorescent probe: SNAP-25B, C-terminally-tagged with monomeric Citrine, connected with a 12 amino acid linker. Expressed in mammalian cells, used with Stx1a-12aa-mCer to measure FRET and dispersion.

    Depositor

    Robert Zucker

    Article

    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

    Insert

    VAMP2 (Vamp2 Rat)
    

    Tags

    12aa-linker and mCitrine

    Expression

    Mammalian

    Available Since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

14. PU.1-ets-IRES-mCherry

    Plasmid

    #80141

    Purpose

    retroviral expression of PU.1-ets (competitive inhibitor of PU.1) and mCherry

    Depositor

    Ellen Rothenberg

    Article

    Kueh et al Science. 2013 Aug 9;341(6146):670-3. doi

    Insert

    PU.1 (Spi1 Mouse)
    

    Use

    Retroviral

    Tags

    IRES-mCherry

    Expression

    Mammalian

    Mutation

    ets = PU.1 DNA binding domain (amino acids 159-26…

    Available Since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGWB443

    Plasmid

    #74829

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with eCFP (no promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Sept. 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. NLS-GAI(1-92)

    Plasmid

    #37317

    Depositor

    Takanari Inoue

    Article

    Miyamoto et al Nat Chem Biol. 2012 Mar 25;8(5):465-70.

    Insert

    GAI (GAI Mustard Weed)
    

    Tags

    NLS

    Expression

    Mammalian

    Mutation

    contains aa 1-92

    Promoter

    CMV

    Available Since

    June 15, 2012

    Availability

    Academic Institutions and Nonprofits only

17. mCer-Vamp2(qv/fw)

    Plasmid

    #53238

    Purpose

    Mutant (Q76V/F77W), tetanus toxin insensitive VAMP2 (synaptobrevin2), N-terminally-tagged with mCerulean, connected by a 4 amino acid linker. For mammalian cells, used with mCit-4aa-SNAP25B for FRET.

    Depositor

    Robert Zucker

    Article

    Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

    Insert

    VAMP2 (Vamp2 Rat)
    

    Tags

    4aa-linker and mCerulean

    Expression

    Mammalian

    Mutation

    Mutated Q76 to V; F77 to W.

    Available Since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

18. pNP1 Aqua toehold switch

    Plasmid

    #107356

    Purpose

    Expresses Aquamarine (an eCFP derivative) in Ecoli off a T7 promoter when pNP1 Aqua trigger is co-expressed

    Depositor

    James Collins

    Article

    Huang et al Sci Adv. 2018 Aug 1;4(8):eaat5105. doi:

    Insert

    toehold switch 8 + Aquamarine
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSL1180-HR-PUbEGFP-NLS

    Plasmid

    #176681

    Purpose

    Expression of nuclear localized EGFP under Ae. aegypti Polyubiquitin promoter (AAEL003888), EGFP version of pSL1180-HR-PUbECFP (Addgene #47917)

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Insert

    EGFP
    

    Expression

    Insect

    Promoter

    Ae. aegypti poly-ubiquitin (AAEL003888)

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPBbsr2-TSen

    Plasmid

    #209916

    Purpose

    A FRET biosensor for ADAM17 activity

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    TSen
    

    Tags

    ECFP and YPet

    Expression

    Mammalian

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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