...default RFP and CFP, while Brainbow 2.1 switches from the default GFP (green) to RFP, CFP, or YFP fluorescence...expressing YFP, CFP, and RFP are not present at the same frequencies. The small population size of CFP++ cells...fluorophores RFP (red), YFP (yellow), and membrane-tethered CFP (cyan). Brainbow 1.1 adds OFP (orange) (Livet et ...to localize to a different area of the cell than CFP and YFP. The most recent derivation of Brainbow,...construct called Zebrabow-GateDest containing an RFP, CFP, and YFP downstream of a ubiquitous promoter. Zebrabow...that randomly express either nuclear GFP, membrane CFP, cytoplasmic YFP, or cytoplasmic RFP in a specific...construct, a variant of Brainbow 1.0 that contains RFP, CFP, and YFP. After crossing onto a tamoxifen-inducible...

...example, CFP (ex 430 nm / em 474 nm) and YFP (ex 514 nm / em 527 nm) can be imaged together but CFP and GFP...for multicolor imaging include ones designed for CFP, YFP, and RFP or the Sedat Quad filter set, designed...

...with significant overlap in their spectra. The pair CFP-YFP was the first to be used to study protein-protein... mCerulean/SYFP2A, mTurquoise/mVenus and others. CFP-YFP is still one of the best and most used pairs ...brightness - even though in the most popular pair, CFP has five-fold less brightness than YFP. The stoichiometry...His tag SCFP3A Cyan Mammalian Express a gene of interest fused to the C-terminus of SCFP3A Amber N1...vector to express a gene of interest fused between ECFP and Venus Other issues that can affect FRET measurements...

...FP. Several early FRET biosensors were made with CFP and YFP, cyan and yellow variants of EGFP equally...equally capable of dimerizing as EGFP. As a result, CFP and YFP dimers can form in the absence of environmental... other GFP family members (superfolder, Emerald, CFP, YFP, BFP, Cerulean, Turquoise, Venus, and Citrine...

...fluorescent proteins: RFP (red), YFP (yellow) and M-CFP (membrane tethered cyan). Without Cre recombination...can mediate one of two deletions to allow YFP or M-CFP expression; these deletions are defined using 2 loxP...express one of four colors (n-GFP, RFP, YFP or M-CFP.) The construct contains two tandem invertible segments...

...mutating GFP, the variants blue FP (BFP), cyan FP (CFP), and yellow FP (YFP) were derived. For a breakdown...FRET occurs when energy transfer from one FP (e.g. CFP) excites the fluorescence of another FP (e.g. YFP...

...signal relative to mCerulean imaged with a standard CFP set. mTFP1 can be used as a FRET donor to mCitrine...fluorescent protein from the coral Clavularia sp. protein CFP484. Hui-wang Ai and colleagues from the University...total of 31 amino acid replacements relative to CFP484. Because the excitation and emission maxima were...was blue-shifted compared to its parent protein CFP484. Collaborating with scientists from Florida State...

... of their system, Tang et al. found that YFP and CFP can activate certain T-DDOG constructs similarly ...

... of the available biosensors use a single GFP or CFP/YFP-derived FRET pair. If you need to free up crowded...

...tagged fusion proteins with fluorescent (YFP, GFP, CFP, RFP, etc.) or affinity tags (HA, FLAG). There are...

...Expression ECFP 433 475 13 4.7 Prone to dimerization pcDNA3-CFP - Mammalian Expression pCAG-CFP - Mammalian...Mammalian Golenbock Lab Plasmids - Includes tagging with CFP, YFP, and mRFP Tsoulfas Lab Lentiviral Plasmids -...moxCerulean3 - Mammalian Expression SCFP3A 433 474 17 4.5 Monomer (A206K) pSCFP3A-C1 - Mammalian Expression mTurquoise2...Vectors - Gateway cloning; includes tagging with ECFP, EYFP, DsRed, Cerulean Bacteria Gradia Lab Bacterial...

...GAL 25Q+ProCFPp303 HTT CFP GAL1 Huntington's Susan Lindquist 15575 GAL 103Q+ProCFPp303 HTT CFP GAL1 Huntington's...Parkinson's Richard Youle 23966 pTreTight-Htt94Q-CFP HTT CFP TREtight Huntington's Nico Dantuma 23971 VCP(... GFP CMV Parkinson's Richard Youle 47560 CFP-Parkin PRKN CFP CMV Parkinson's Richard Youle 48685 pBabe...15578 GAL HA25QDProCFPp303 HTT HA, CFP GAL1 Huntington's Susan Lindquist 15579 GAL HA103QDProCFPp303 HTT HA...Huntington's Susan Lindquist 15570 GAL HA25Q+ProCFPp303 HTT HA, CFP GAL1 Huntington's Susan Lindquist 15572...Huntington's Susan Lindquist 15584 GAL FLAG25QDProCFPp303 HTT FLAG, CFP GAL1 Huntington's Susan Lindquist ...Lindquist 15585 GAL FLAG46QDProCFPp303 HTT FLAG, CFP GAL1 Huntington's Susan Lindquist 15586 GAL FLAG72QDProCFPp303...

...containing 3-AT. FLIParaF.Ec-250n 18855 N/A FRET between CFP and YFP Wolf Frommer Reporter for arabinose levels...presence of arabinose enhances the FRET signal from the CFP and YFP fluorophores linked by an arabinose binding...

...Acceptor Tag R0 (Å)a Range (Å)b Applications CFP   Lumio Green Tc: CCPGCC 50 40-72 Intact Cells ...

...pcDNA3.1-CMV-CFP;UBC-Cre25nt Cre with a 25 nucleotide extracellular vesicle targeting sequence and CFP UBC Mammalian...

...mGold Francois St-Pierre 14873 GolgiCFP Golgi apparatus eNOS(1-33) CFP Alexandra Newton 36205 pmTurquoise2...

...Kamiyama D. Commun Biol. 2021 Daichi Kamiyama ECFP C155 of ECFP pairs with N173 derived from Cerulean or Venus...

...V68L; Q69K; S72A; T203Y ECFP F64L; S65T, Y66W; N146I; M153T; V163A mCFP F64L; S65T, Y66W; N146I; M153T...

...variants have been discovered such as Kaede and PS-CFP2 (see Table 1). Table 1: Properties of Selected Irreversible... UV-Violet Dark/ Green ~200 Medium 504/ 517 PS-CFP2 Monomer UV-Violet Cyan/ Green >2,000 Medium 490/...

...C-terminal His tag SCFP3A Cyan Mammalian Express a gene of interest fused to the C-terminus of SCFP3A Amber N1 ... ECFP and EYFP connected by 8 GGSGGS repeats pET28CLY9 Peptide linker standard consisting of ECFP and ...vector to express a gene of interest fused between ECFP and Venus Back to Top FRET Reference Standards The... pET28CLY1 Peptide linker standard consisting of ECFP and EYFP connected by 1 flexible glycine- and serine-containing... pET28CLY2 Peptide linker standard consisting of ECFP and EYFP connected by 2 GGSGGS repeats pET28CLY3...pET28CLY3 Peptide linker standard consisting of ECFP and EYFP connected by 3 GGSGGS repeats pET28CLY4 Peptide...Peptide linker standard consisting of ECFP and EYFP connected by 4 GGSGGS repeats pET28CLY5 Peptide linker ...