Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 14 results for: cfp

Showing: 1 - 14 of 14 results

  1. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

    Type
    Blog Post
    Published
    March 5, 2019, 1:08 p.m.
    ...default RFP and CFP, while Brainbow 2.1 switches from the default GFP (green) to RFP, CFP, or YFP fluorescence...expressing YFP, CFP, and RFP are not present at the same frequencies. The small population size of CFP++ cells...fluorophores RFP (red), YFP (yellow), and membrane-tethered CFP (cyan). Brainbow 1.1 adds OFP (orange) (Livet et ...to localize to a different area of the cell than CFP and YFP. The most recent derivation of Brainbow,...construct called Zebrabow-GateDest containing an RFP, CFP, and YFP downstream of a ubiquitous promoter. Zebrabow...that randomly express either nuclear GFP, membrane CFP, cytoplasmic YFP, or cytoplasmic RFP in a specific...construct, a variant of Brainbow 1.0 that contains RFP, CFP, and YFP. After crossing onto a tamoxifen-inducible...

  2. Choosing Your Fluorescent Proteins for Multi-Color Imaging

    Type
    Blog Post
    Published
    Oct. 9, 2014, 3 p.m.
    ...example, CFP (ex 430 nm / em 474 nm) and YFP (ex 514 nm / em 527 nm) can be imaged together but CFP and GFP...for multicolor imaging include ones designed for CFP, YFP, and RFP or the Sedat Quad filter set, designed...

  4. Tips for Using FRET in Your Experiments

    Type
    Blog Post
    Published
    Nov. 5, 2014, 3:41 p.m.
    ...with significant overlap in their spectra. The pair CFP-YFP was the first to be used to study protein-protein... mCerulean/SYFP2A, mTurquoise/mVenus and others. CFP-YFP is still one of the best and most used pairs ...brightness - even though in the most popular pair, CFP has five-fold less brightness than YFP. The stoichiometry...His tag SCFP3A Cyan Mammalian Express a gene of interest fused to the C-terminus of SCFP3A Amber N1...vector to express a gene of interest fused between ECFP and Venus Other issues that can affect FRET measurements...

  5. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...fluorescent proteins: RFP (red), YFP (yellow) and M-CFP (membrane tethered cyan). Without Cre recombination...can mediate one of two deletions to allow YFP or M-CFP expression; these deletions are defined using 2 loxP...express one of four colors (n-GFP, RFP, YFP or M-CFP.) The construct contains two tandem invertible segments...

  6. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...mutating GFP, the variants blue FP (BFP), cyan FP (CFP), and yellow FP (YFP) were derived. For a breakdown...FRET occurs when energy transfer from one FP (e.g. CFP) excites the fluorescence of another FP (e.g. YFP...

  7. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...signal relative to mCerulean imaged with a standard CFP set. mTFP1 can be used as a FRET donor to mCitrine...fluorescent protein from the coral Clavularia sp. protein CFP484. Hui-wang Ai and colleagues from the University...total of 31 amino acid replacements relative to CFP484. Because the excitation and emission maxima were...was blue-shifted compared to its parent protein CFP484. Collaborating with scientists from Florida State...

  8. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    Published
    Aug. 10, 2023, 1 p.m.
    ... of the available biosensors use a single GFP or CFP/YFP-derived FRET pair. If you need to free up crowded...

  10. Tips for arabidopsis transformation

    Type
    Blog Post
    Published
    Oct. 25, 2018, 1:23 p.m.
    ...tagged fusion proteins with fluorescent (YFP, GFP, CFP, RFP, etc.) or affinity tags (HA, FLAG). There are...

  14. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...variants have been discovered such as Kaede and PS-CFP2 (see Table 1). Table 1: Properties of Selected Irreversible... UV-Violet Dark/ Green ~200 Medium 504/ 517 PS-CFP2 Monomer UV-Violet Cyan/ Green >2,000 Medium 490/...
Showing: 1 - 14 of 14 results