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Tuning of Recombinant Protein Expression in Escherichia coli by Manipulating Transcription, Translation Initiation Rates, and Incorporation of Noncanonical Amino Acids.
Schlesinger O, Chemla Y, Heltberg M, Ozer E, Marshall R, Noireaux V, Jensen MH, Alfonta L
ACS Synth Biol. 2017 Mar 9. doi: 10.1021/acssynbio.7b00019. PubMed Article

Plasmids from Article

ID Plasmid Purpose
92222pBEST-p70b-UTR1-deGFP-6xHis-T500Expression of deGFP using the p70b promoter (weak version of the p70a promoter)
92224pBEST-p70a-UTR3-deGFP-6xHis-T500Expression of deGFP using the p70a promoter and UTR3
92225pBEST-p70a-UTR1-deGFP Y35TAG-6xHis-T500Expression of deGFP mutant Y35TAG for ncAA incoporation using the p70a promoter and UTR1
92227pBEST-p70a-UTR1-deGFP Y35TAG D193TAG-6xHis-T500Expression of deGFP mutant Y35TAG D193TAG for ncAA incoporation using the p70a promoter and UTR1
92228pBEST-p70a-UTR1-NTerm 6xHis-zmADH-T500Expression of zmADH using the p70a promoter and UTR1
92229pBEST-p70b-UTR1-NTerm 6xHis-zmADH-T500Expression of zmADH using the p70b (weak version of the p70a promoter) promoter and UTR1
92230pBEST-p70a-UTR1-NTerm 6xHis-zmADH V86TAG-T500Expression of zmADH mutant V86TAG for ncAA incoporation using the p70a promoter and UTR1
92314pBEST-p70a-UTR1-Nterm 6xHis PL-T500Expression of NTerm 6xHis tagged protein L (PL) domain using the p70a promoter and UTR1
92315pBEST-p70a-UTR1-Nterm 6xHis PL K16TAG-T500Expression of NTerm 6xHis tagged protein L (PL) mutant K16TAG for ncAA incoporation using the p70a promoter and UTR1
92316pBEST-p70a-UTR1-Nterm 6xHis mRFP-T500Expression of NTerm 6xHis tagged mRFP using the p70a promoter and UTR1
92317pBEST-p70a-UTR3-Nterm 6xHis mRFP-T500Expression of NTerm 6xHis tagged mRFP using the p70a promoter and UTR3
92318pBEST-p70a-UTR1-Nterm 6xHis mRFP K15TAG-T500Expression of NTerm 6xHis tagged mRFP mutant K15TAG for ncAA incoporation using the p70a promoter and UTR1
92319pBEST-p70a-UTR3-Nterm 6xHis mRFP K15TAG-T500Expression of NTerm 6xHis tagged mRFP mutant K15TAG for ncAA incoporation using the p70a promoter and UTR3

Antibodies from Article