We narrowed to 9,520 results for: try

1. pTALdNC-AAVS1_T2

   Plasmid

   #80496

   Purpose

   AAVS1 TALEN expression vector (Right)

   Depositor

   Knut Woltjen

   Article

   Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

   Insert

   AAVS1 TALEN (Right)
   

   Use

   Gateway entry

   Promoter

   none

   Available Since

   Nov. 28, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLiLucB

   Plasmid

   #31596

   Depositor

   Michele Calos

   Article

   Woodard et al PLoS One. 2010 . 5(6):e11367.

   Insert

   alpha-1 antitrypsin promoter with an apolipoprotein enhancer (SERPINA1 Human)
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   Aug. 18, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcs2-GFP-IRES-mCherry

   Plasmid

   #226098

   Purpose

   Protein stability reporter construct for transient expression in mammalian cells. Stability of GFP-fusion protein can be assed by flow cytometry by normalizing to mCherry expression.

   Depositor

   Michael Rape

   Article

   Haakonsen et al Nature. 2024 Feb;626(8000):874-880. doi:

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Oct. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBP-lacZ

   Plasmid

   #72948

   Purpose

   Entry vector for Bioparts - Oligo anneal or PCR with internal BsaI sites at 5' and 3' ends using NdeI/SphI cloning

   Depositor

   Paul Freemont

   Article

   Moore et al ACS Synth Biol. 2016 May 2.

   Insert

   Level 0 - Bioparts
   

   Use

   Synthetic Biology

   Mutation

   CAT gene - C435G (nucleotide) - silent mutagenesi…

   Available Since

   July 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. TurboID-mNeongreen-3xFLAG fusion pos3

   Plasmid

   #172864

   Purpose

   C. elegans codon-optimized gateway entry vector expressing TurboID-mNeongreen fusion protein

   Depositor

   Mario de Bono

   Article

   Artan et al J Biol Chem. 2021 Sep;297(3):101094. doi

   Insert

   TurboID
   

   Use

   Unspecified

   Tags

   FLAG, mNeongreen, and myc

   Available Since

   Oct. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. p5E-EF1a/b-actin

   Plasmid

   #82583

   Purpose

   5' entry vector containing the EF1a/B-globin fused to zebrafish b-actin 2 enhancer/promoter for strong semi-ubiquitous expression

   Depositor

   Kryn Stankunas

   Article

   Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

   Insert

   EF1a/b-globin fused to zebrafish b-actin 2 enhancer/promoter
   

   Promoter

   None

   Available Since

   Dec. 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pD-MSP1D1

   Plasmid

   #161607

   Purpose

   Bacterial expression of mutated membrane scaffold protein ("dark" MSP1D1) for Nanodisc formation

   Depositor

   Stephen Sligar

   Article

   McLean et al Anal Biochem. 2020 Oct 15;607:113860. do

   Insert

   D-MSP1D1
   

   Tags

   7-His tag

   Expression

   Bacterial

   Mutation

   mutant of MSP1D1; all tryptophans were replaced b…

   Promoter

   T7

   Available Since

   Nov. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. NmCas9_sgRNA_expression_in_pBluescript

   Plasmid

   #122091

   Purpose

   U6 driven NmCas9 sgRNA expression vector for cloning own guides

   Depositor

   Scot Wolfe

   Article

   Bolukbasi et al Nat Commun. 2018 Nov 19;9(1):4856. doi:

   Insert

   NmCas9 tracrRNA (NEWENTRY Synthetic, S. pyogenes)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCx55.5-TrpR

   Plasmid

   #86373

   Purpose

   for zebrafish transgenesis, expression of TrpR from Cx55.5 promoter, also expresses mCherry

   Depositor

   Rachel Wong

   Article

   Yoshimatsu et al Nat Commun. 2016 Feb 3;7:10590. doi: 10.

   Insert

   TrpR
   

   Use

   Zebrafish

   Promoter

   cx55.5

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHAGE-EF1alphaL-hAAT-W

    Plasmid

    #24527

    Purpose

    Lentiviral overexpression

    Depositor

    Darrell Kotton

    Article

    Wilson et al J Clin Invest. 2010 Jan . 120(1):379-89.

    Insert

    alpha-1 antitrypsin (SERPINA1 Human)
    

    Use

    Lentiviral

    Available Since

    April 30, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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