We narrowed to 15,769 results for: LEA

1. pET-28a(+)-His(6)-TEV-AtPCO4-2(wt)

   Plasmid

   #191802

   Purpose

   Full length wt AtPCO4-2 with Nt-His tag

   Depositor

   Emily Flashman

   Article

   White et al J Biol Chem. 2018 Jul 27;293(30):11786-1

   Insert

   AtPCO4-2
   

   Tags

   6xHis, TEV cleavage site

   Expression

   Bacterial

   Available Since

   April 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAGEN_NES_V5_GFP

   Plasmid

   #235632

   Purpose

   Encodes V5-GFP with an N-terminal nuclear export sequence. Used as a no-ALIBi negative control.

   Depositor

   Maria Barna

   Article

   Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

   Insert

   GFP
   

   Tags

   NES-V5

   Expression

   Mammalian

   Available Since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. JDW 1061 (pLenti6.2_CMV_GATA1_P2A_H2A_mCherry)

   Plasmid

   #233543

   Purpose

   A CMV driven human GATA1 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

   Depositor

   Joshua Wythe

   Article

   Cantu Gutierrez et al bioRxiv 2021.11.15.468651

   Insert

   GATA1 (GATA1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Available Since

   March 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAG415Gal_Gcn4 solvvol

   Plasmid

   #232976

   Purpose

   Galactose iduced expression of Gcn4 solvvol in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 solvvol
   

   Tags

   TEV cleavage site

   Expression

   Yeast

   Mutation

   S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

   Promoter

   Gal1

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAG415Gal_Gcn4 ILVtoED WHA

   Plasmid

   #232995

   Purpose

   Galactose iduced expression of Gcn4 ILVtoED W+HAin yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 ILVtoED W+
   

   Tags

   3xHA and TEV cleavage site

   Expression

   Yeast

   Mutation

   N126W, I128E, V130E, V135E, L137D, I142D

   Promoter

   Gal1

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAG415Gal_Gcn4 9acidAHA

   Plasmid

   #232997

   Purpose

   Galactose iduced expression of Gcn4 9acidAHA in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 9acidA
   

   Tags

   3xHA and TEV cleavage site

   Expression

   Yeast

   Mutation

   E109A, E111A, E114A, D115A, E119A, D125A, D127A, …

   Promoter

   Gal1

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAG415Gal_Gcn4 9acidAGFP

   Plasmid

   #233009

   Purpose

   Galactose iduced expression of Gcn4 9acidAGFP in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 9acidA
   

   Tags

   HA-GFP-HA and TEV cleavage site

   Expression

   Yeast

   Mutation

   E109A, E111A, E114A, D115A, E119A, D125A, D127A, …

   Promoter

   Gal1

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAG415Gal_Gcn4 ILVtoED WGFP

   Plasmid

   #233007

   Purpose

   Galactose iduced expression of Gcn4 LVtoED W+GFPin yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 ILVtoED W+
   

   Tags

   HA-GFP-HA and TEV cleavage site

   Expression

   Yeast

   Mutation

   N126W, I128E, V130E, V135E, L137D, I142D

   Promoter

   Gal1

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAG415Gal_Gcn4 solvvol W

   Plasmid

   #232977

   Purpose

   Galactose iduced expression of Gcn4 solvvol W+ in yeast

   Depositor

   Aseem Ansari, Tanja Mittag

   Article

   Bremer et al bioRxiv 2024.11.21.624739

   Insert

   Gcn4 solvvol W+
   

   Tags

   3xHA and TEV cleavage site

   Expression

   Yeast

   Mutation

   S101T, D103E, S104T, D115E, N116Q, S117T, S122T, …

   Promoter

   Gal1

   Available Since

   March 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. GB4003

    Plasmid

    #215248

    Purpose

    Module for the constitutive expression of PIF6 and PhyB:VP16

    Depositor

    Diego Orzaez

    Article

    Calvache et al Plant Biotechnol J. 2024 Jan;22(1):37-47

    Insert

    PIF6-PhyB:VP16
    

    Use

    Synthetic Biology

    Mutation

    V234L in PIF6- please see dep. sequence, BsaI and…

    Available Since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDest17_Gcn4 SATtoG KtoR

    Plasmid

    #231861

    Purpose

    Bacterial expression of N-terminally 6His tagged Gcn4 SATtoG KtoR

    Depositor

    Aseem Ansari, Tanja Mittag

    Article

    Bremer et al bioRxiv 2024.11.21.624739

    Insert

    Gcn4 SATtoG KtoR
    

    Tags

    6xHis, TEV cleavage site

    Expression

    Bacterial

    Mutation

    S101G, T102G, S104G, T105G, S117G, K118R, T121G, …

    Promoter

    T7

    Available Since

    March 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. JDW 1394 (pME-ZIC3-P2A-H2A-mCherry)

    Plasmid

    #232133

    Purpose

    A middle entry gateway clone containing V5 tagged human ZIC3 and an H2A tagged mCherry, separated by a P2A peptide cleavage sequence.

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    ZIC3 (ZIC3 Human)
    

    Use

    Gateway cloning

    Tags

    V5

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. JDW 1063 (pLenti6.2_CMV_V5_MAFB_P2A_H2A_mCherry)

    Plasmid

    #233545

    Purpose

    A CMV driven human MAFB followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    MAFB (MAFB Human)
    

    Use

    Lentiviral

    Tags

    V5

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. JDW 1473 (pLenti-BLAST-ZIC3-P2A-H2A-mCherry)

    Plasmid

    #233547

    Purpose

    A CMV driven human ZIC3 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

    Depositor

    Joshua Wythe

    Article

    Cantu Gutierrez et al bioRxiv 2021.11.15.468651

    Insert

    ZIC3 (ZIC3 Human)
    

    Use

    Lentiviral

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA-Sga-Cas9

    Plasmid

    #227005

    Purpose

    Mammalian expression of Nuclease active S. gallolyticus Cas9

    Depositor

    Charles Gersbach

    Article

    Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA-Sin-Cas9

    Plasmid

    #227006

    Purpose

    Mammalian expression of Nuclease active S. iniae Cas9

    Depositor

    Charles Gersbach

    Article

    Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA-Spa-Cas9

    Plasmid

    #227007

    Purpose

    Mammalian expression of Nuclease active S. parasanguinis Cas9

    Depositor

    Charles Gersbach

    Article

    Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA-Sub-Cas9

    Plasmid

    #227008

    Purpose

    Mammalian expression of Nuclease active S. uberis Cas9

    Depositor

    Charles Gersbach

    Article

    Butterfield et al Proc Natl Acad Sci U S A. 2025 Mar 18;12

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 14, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDF-His_TEV_MsbA

    Plasmid

    #191476

    Purpose

    Expresses MsbA with a TEV protease cleavable N terminal His Tag

    Depositor

    Arthur Laganowsky

    Article

    Lyu et al Nat Commun. 2022 Nov 26;13(1):7291. doi:

    Insert

    MsbA
    

    Tags

    His

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET28a-hfYFP-TEV-Bcx

    Plasmid

    #191195

    Purpose

    Fusion of hfYFP and Bacillus circulans xylanase separated by a TEV cleavage site for fluorescence-assisted protein purification

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    hfYFP & Bcx fusion
    

    Tags

    6xHis and Bacillus circulans xylanase

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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