We narrowed to 4,504 results for: pcr

1. pX20

   Plasmid

   #169443

   Purpose

   Used for guide cloning with PCR

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pX52

   Plasmid

   #169444

   Purpose

   Used for guide cloning with PCR

   Depositor

   Richard Huganir

   Article

   Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPL5617_pUG_PuroR

   Plasmid

   #60928

   Purpose

   PCR template vector for generating puromycin resistance cassette.

   Depositor

   Robert Piper

   Article

   MacDonald et al Yeast. 2015 Feb 16. doi: 10.1002/yea.306

   Insert

   puromycin N-Acetyltransferase
   

   Use

   Loxp flanked deletion cassette template

   Tags

   Expression

   Mutation

   Promoter

   TEF1

   Available since

   Feb. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pKM342

   Plasmid

   #71486

   Purpose

   Contains loxP-hyg-loxP cassette for recombineering; HygR, AmpR.

   Depositor

   Kenan Murphy

   Article

   Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:

   Insert

   loxP-hyg-loxP cassette
   

   Use

   Source of loxp-hyg-loxp for pcr amplification.

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pKM343

   Plasmid

   #71487

   Purpose

   Contains loxP-hyg-cat-loxP cassette for recombineering; CamR, HygR.

   Depositor

   Kenan Murphy

   Article

   Murphy et al Methods Mol Biol. 2015;1285:177-99. doi:

   Insert

   loxP-hyg-cat-loxP
   

   Use

   Source of loxp-hyg-cat- loxp for pcr amplificatio…

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pBSWt1-Dest

   Plasmid

   #59764

   Purpose

   PCR template for amplifying the attL1-em7-kanr-attL2-pA cassette

   Depositor

   Peter Koopman

   Article

   Zhao et al Dev Dyn. 2014 Jun 12. doi: 10.1002/dvdy.

   Insert

   attL1-em7-kanR-attL2-pA
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pNR1D2-donor

   Plasmid

   #112314

   Purpose

   CRISPR donor plasmid to tag human transcription factor NR1D2 with GFP

   Depositor

   Kevin White

   Insert

   NR1D2 homology arms flanking EGFP-IRES-Neo cassette (NR1D2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   hg38:3:23977483:A>T, hg38:3:23977515:A>G, h…

   Promoter

   Available since

   Nov. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. mUAV

   Plasmid

   #102680

   Purpose

   Mobius Assembly Universal Acceptor Vector, it converts amplified PCR fragments into standard, exchangeable parts

   Depositor

   Naomi Nakayama

   Article

   Andreou et al PLoS One. 2018 Jan 2;13(1):e0189892. doi

   Insert

   J23106:B0034:amilCP:rrnBT1-T7TE
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. FRP2375

   Plasmid

   #127581

   Purpose

   Tagging plasmid for WTC 846: Encodes the PtetO7.1 promoter and NAT resistance gene

   Depositor

   Joerg Stelling

   Article

   Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

   Insert

   PtetO7.1_Flagtag_8Glycine linker
   

   Use

   Synthetic Biology; Pcr based tagging

   Tags

   Flag tag, 8glycine linker

   Expression

   Mutation

   Promoter

   Available since

   Aug. 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. FRP2350

    Plasmid

    #127580

    Purpose

    Tagging plasmid for WTC 846: Encodes the PtetO7.1 promoter and Hyg resistance gene

    Depositor

    Joerg Stelling

    Article

    Azizoglu et al Elife. 2021 Aug 3;10. pii: 69549. doi: 1

    Insert

    PtetO7.1_Flagtag_8Glycine linker
    

    Use

    Synthetic Biology; Pcr based tagging

    Tags

    Flag tag, 8glycine linker

    Expression

    Mutation

    Promoter

    Available since

    Aug. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEPkan-S2

    Plasmid

    #61601

    Purpose

    PCR template for en passant (two-step Red-mediated) mutagenesis

    Depositor

    Nikolaus Osterrieder

    Article

    Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

    Insert

    Kana_I-SceI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEPkan-S

    Plasmid

    #41017

    Purpose

    PCR template for en passant (two-step Red-mediated) mutagenesis

    Depositor

    Nikolaus Osterrieder

    Article

    Tischer et al Biotechniques. 2006 Feb;40(2):191-7.

    Insert

    Kana_I-SceI
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. mg409

    Plasmid

    #73368

    Purpose

    unc-70 no-force control

    Depositor

    Miriam Goodman

    Article

    Krieg et al Nat Cell Biol. 2014 Mar;16(3):224-33. do

    Insert

    TSMod::unc-70 (unc-70 Nematode)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    unc-70p

    Available since

    Feb. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pATF4-donor

    Plasmid

    #113805

    Purpose

    CRISPR donor plasmid to tag human transcription factor ATF4 with GFP

    Depositor

    Kevin White

    Insert

    ATF4 homology arms flanking EGFP-IRES-Neo cassette (ATF4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    rs140428747

    Promoter

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFA CRU HISMX

    Plasmid

    #131164

    Purpose

    PCR template for genomic C-terminal Cub-R-URA3 tag, HISMX complements the histidine auxotrophy of S. cerevisiae his mutant

    Depositor

    Nils Johnsson

    Article

    Dunkler et al Methods Mol Biol. 2012;786:115-30. doi:

    Type

    Empty backbone

    Use

    Tags

    CRU

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCfB8739

    Plasmid

    #126905

    Purpose

    Template for PCR amplification of XdCrtYB

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    XdCrtYB
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCfB8740

    Plasmid

    #126906

    Purpose

    Template for PCR amplification of XdCrtI

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    XdCrtI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCfB8741

    Plasmid

    #126907

    Purpose

    Template for PCR amplification of XdCrtE

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    XdCrtE
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. XE85 Delta Beta-catenin in GFP

    Plasmid

    #16835

    Depositor

    Randall Moon

    Insert

    Delta beta-catenin GFP (ctnnb1.L Frog)
    

    Use

    Xenopus expression

    Tags

    GFP

    Expression

    Mutation

    These PCR fragments have the first 138 bp after t…

    Promoter

    Available since

    March 13, 2008

    Availability

    Academic Institutions and Nonprofits only

20. pCfB8818

    Plasmid

    #126909

    Purpose

    Template for PCR amplification of HMG1from Yarrowia lipolytica

    Depositor

    Irina Borodina

    Article

    Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

    Insert

    YlHMG1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge