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TFORF3114

Plasmid

#144590

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

BHLHA15 (BHLHA15 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF3122

Plasmid

#144598

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

SATB1 (SATB1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF3080

Plasmid

#144556

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ZNF296 (ZNF296 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF3029

Plasmid

#144505

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

ATOH1 (ATOH1 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF3041

Plasmid

#144517

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

SOX15 (SOX15 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF2963

Plasmid

#144439

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

NR2C2 (NR2C2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST-GST-FBW7 alpha

Plasmid

#197456

Purpose

The plasmid expresses GST-tagged FBW7 human isoform 1. Used for in vitro translation of FBW7alpha.

Depositor

Omar Khan

Article

Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

Insert

FBW7 (FBXW7 Human)

Use

In vitro translation

Tags

GST

Expression

Bacterial

Mutation

See depositor comments below

Promoter

T7

Available Since

Aug. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMT405

Plasmid

#139383

Purpose

LacZ, pNBU1 backbone with BsaI sites, AmpR, ErmR

Depositor

Christopher Voigt

Article

Taketani et al Nat Biotechnol. 2020 Aug;38(8):962-969.

Insert

LacZ

Use

Synthetic Biology

Promoter

No promoter

Available Since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti6.3/TO/V5-DEST-IGFBP2-Clover

Plasmid

#191008

Purpose

Lentiviral expression vector for IGFBP2 with a fluorescent Clover tag

Depositor

Johanna Ivaska

Article

Conway et al Sci Adv. 2023 Jul 14;9(28):eadg1840. doi

Insert

Insulin-Like Growth Factor Binding Protein 2 (IGFBP2 Human)

Use

Lentiviral

Tags

Clover

Promoter

CMV

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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tetO-HEY2

Plasmid

#170691

Purpose

doxycycline-inducible overexpression of HEY2

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

HEY2 – hes related family bHLH transcription factor with YRPW motif 2 (HEY2 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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tetO-AKT1

Plasmid

#170682

Purpose

doxycycline-inducible overexpression of AKT1

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

AKT1 (AKT1 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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tetO-FHL2

Plasmid

#170686

Purpose

doxycycline-inducible overexpression of FHL2

Depositor

Paul Burridge

Article

Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

Insert

FHL2 (FHL2 Human)

Use

Lentiviral; Doxycycline inducible

Expression

Mammalian

Promoter

TRE promoter, Tet-ON

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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IDG_BEST4_OE_1

Plasmid

#161678

Purpose

Overexpresses BEST4

Depositor

Mike McManus

Insert

BEST4 (BEST4 Human)

Tags

V5

Expression

Mammalian

Available Since

June 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCFJ108 - pENTR221 - [cbr-unc-119(+)]

Plasmid

#200367

Purpose

cbr-unc-119 rescue gene for use as co-injection marker.

Depositor

Christian Froekjaer-Jensen

Article

AlHarbi et al MicroPubl Biol. 2023 Jun 22;2023:10.1791

Insert

[cbr-unc-119(+)]

Expression

Worm

Available Since

June 16, 2023

Availability

Academic Institutions and Nonprofits only

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pDEST-Myc-FBW7 Delta F-Box

Plasmid

#197452

Purpose

The plasmid expresses F-box deleted version of Myc-tagged FBW7 human isoform 1. Used for mammalian overexpression of FBW7alpha delta-F-Box and detection by western blotting.

Depositor

Omar Khan

Article

Khan et al Nat Commun. 2021 Apr 6;12(1):2043. doi:

Insert

FBW7 (FBXW7 Human)

Tags

Myc

Expression

Mammalian

Mutation

FBW7 Fbox deleted.

Promoter

CMV

Available Since

June 1, 2023

Availability

Academic Institutions and Nonprofits only

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BAF-WT-tet-puro

Plasmid

#199648

Purpose

Doxycycline-inducible BAF expression

Depositor

William Hahn

Article

So et al JCI Insight. 2022 Oct 10;7(19):e158755.

Insert

BAF (BANF1 Human)

Use

Lentiviral

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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dpYPQ230-D156R-CBE2

Plasmid

#195368

Purpose

dLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of XTEN

Depositor

Yiping Qi

Article

Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

Insert

hA3A/Y130F-XTEN-dLbCas12a-D156R-UGI

Use

CRISPR; Gateway compatible ha3a/y130f-xten-dlbcas…

Expression

Plant

Promoter

ZmUBI1

Available Since

May 17, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-hTLR3-VenusFlag

Plasmid

#197636

Purpose

Human CD283 protein expression in mammalian cells

Depositor

Haian Fu

Insert

TLR3 (TLR3 Human)

Tags

Venus-Flag

Expression

Mammalian

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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TFORF3082

Plasmid

#144558

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

GBX2 (GBX2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

May 10, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA3-hTICAM1-VenusFlag

Plasmid

#197632

Purpose

Human TRIF protein expression in mammalian cells

Depositor

Haian Fu

Insert

TICAM1 (TICAM1 Human)

Tags

Venus-Flag

Expression

Mammalian

Available Since

May 9, 2023

Availability

Academic Institutions and Nonprofits only

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